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Optimized DNA extraction from fecal samples using non-stool kits to improve PCR detection of protozoan parasites: A focus on Blastocystis sp.

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Title: Optimized DNA extraction from fecal samples using non-stool kits to improve PCR detection of protozoan parasites: A focus on Blastocystis sp.
Authors: Guilane A; Laboratory of Biodiversity and Environment: Interactions - Genomes, Faculty of Biological Sciences, University of Science and Technology Houari Boumediene, 16111 Algiers, Algeria; Laboratory of Parasitic Eco-epidemiology and Population Genetics, Pasteur Institute of Algeria, Dely-Brahim, 16047, Algiers, Algeria., Medrouh B; Center of Research in Agro-Pastoralism, 17000 Djelfa, Algeria., Zait H; Laboratory of Parasitic Eco-epidemiology and Population Genetics, Pasteur Institute of Algeria, Dely-Brahim, 16047, Algiers, Algeria., Haleche I; Laboratory of Biotechnology, Environment and Health, University of Blida 1, Blida, Algeria., Boutellis A; Laboratory of Biodiversity and Environment: Interactions - Genomes, Faculty of Biological Sciences, University of Science and Technology Houari Boumediene, 16111 Algiers, Algeria., Kernif T; Laboratory of Parasitic Eco-epidemiology and Population Genetics, Pasteur Institute of Algeria, Dely-Brahim, 16047, Algiers, Algeria. Electronic address: kernif.tahar@gmail.com.
Source: Journal of microbiological methods [J Microbiol Methods] 2026 Jun; Vol. 245, pp. 107485. Date of Electronic Publication: 2026 Apr 06.
Publication Type: Journal Article
Journal Info: Publisher: Elsevier Biomedical Country of Publication: Netherlands NLM ID: 8306883 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-8359 (Electronic) Linking ISSN: 01677012 NLM ISO Abbreviation: J Microbiol Methods Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: Optimized DNA extraction from fecal samples using non-stool kits to improve PCR detection of protozoan parasites: A focus on Blastocystis sp.
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  Data: <searchLink fieldCode="AU" term="%22Guilane+A%22">Guilane A</searchLink>; Laboratory of Biodiversity and Environment: Interactions - Genomes, Faculty of Biological Sciences, University of Science and Technology Houari Boumediene, 16111 Algiers, Algeria; Laboratory of Parasitic Eco-epidemiology and Population Genetics, Pasteur Institute of Algeria, Dely-Brahim, 16047, Algiers, Algeria.<br /><searchLink fieldCode="AU" term="%22Medrouh+B%22">Medrouh B</searchLink>; Center of Research in Agro-Pastoralism, 17000 Djelfa, Algeria.<br /><searchLink fieldCode="AU" term="%22Zait+H%22">Zait H</searchLink>; Laboratory of Parasitic Eco-epidemiology and Population Genetics, Pasteur Institute of Algeria, Dely-Brahim, 16047, Algiers, Algeria.<br /><searchLink fieldCode="AU" term="%22Haleche+I%22">Haleche I</searchLink>; Laboratory of Biotechnology, Environment and Health, University of Blida 1, Blida, Algeria.<br /><searchLink fieldCode="AU" term="%22Boutellis+A%22">Boutellis A</searchLink>; Laboratory of Biodiversity and Environment: Interactions - Genomes, Faculty of Biological Sciences, University of Science and Technology Houari Boumediene, 16111 Algiers, Algeria.<br /><searchLink fieldCode="AU" term="%22Kernif+T%22">Kernif T</searchLink>; Laboratory of Parasitic Eco-epidemiology and Population Genetics, Pasteur Institute of Algeria, Dely-Brahim, 16047, Algiers, Algeria. Electronic address: kernif.tahar@gmail.com.
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  Data: <searchLink fieldCode="JN" term="%228306883%22">Journal of microbiological methods</searchLink> [J Microbiol Methods] 2026 Jun; Vol. 245, pp. 107485. <i>Date of Electronic Publication: </i>2026 Apr 06.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Elsevier+Biomedical%22">Elsevier Biomedical </searchLink><i>Country of Publication: </i>Netherlands <i>NLM ID: </i>8306883 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1872-8359 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2201677012%22">01677012 </searchLink><i>NLM ISO Abbreviation: </i>J Microbiol Methods <i>Subsets: </i>MEDLINE
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        Value: 10.1016/j.mimet.2026.107485
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      – TitleFull: Optimized DNA extraction from fecal samples using non-stool kits to improve PCR detection of protozoan parasites: A focus on Blastocystis sp.
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              Text: 2026 Jun
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