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Comprehensive guide for optimizing octopus immune cell preparation to enhance single cell RNA sequencing success.

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Title: Comprehensive guide for optimizing octopus immune cell preparation to enhance single cell RNA sequencing success.
Authors: Costa MM; Instituto de Investigaciones Marinas (IIM), CSIC, Vigo, Spain., Ferreirós-Vidal I; Genética de Poblaciones en Biomedicina (GenPoB) Research Group, Facultade de Medicina, Instituto de Ciencias Forenses, Instituto de Investigación Sanitaria (IDIS), Unidade de Xenética, Universidade de Santiago de Compostela, Hospital Clínico Universitario de Santiago (SERGAS), 15706, Galicia, Spain.; Genetics, Vaccines and Infections Research Group (GENVIP), Instituto de Investigación Sanitaria de Santiago, Santiago de Compostela, Galicia, Spain.; Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBER-ES), Madrid, Spain., Salas A; Genética de Poblaciones en Biomedicina (GenPoB) Research Group, Facultade de Medicina, Instituto de Ciencias Forenses, Instituto de Investigación Sanitaria (IDIS), Unidade de Xenética, Universidade de Santiago de Compostela, Hospital Clínico Universitario de Santiago (SERGAS), 15706, Galicia, Spain.; Genetics, Vaccines and Infections Research Group (GENVIP), Instituto de Investigación Sanitaria de Santiago, Santiago de Compostela, Galicia, Spain.; Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBER-ES), Madrid, Spain., Dios S; Instituto de Investigaciones Marinas (IIM), CSIC, Vigo, Spain., Gambón F; Department of Immunology, Vigo University Hospital Complex, Vigo, Spain., Gestal C; Instituto de Investigaciones Marinas (IIM), CSIC, Vigo, Spain. cgestal@iim.csic.es.
Source: Scientific reports [Sci Rep] 2026 Apr 10; Vol. 16 (1). Date of Electronic Publication: 2026 Apr 10.
Publication Type: Journal Article
Journal Info: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: <searchLink fieldCode="AU" term="%22Costa+MM%22">Costa MM</searchLink>; Instituto de Investigaciones Marinas (IIM), CSIC, Vigo, Spain.<br /><searchLink fieldCode="AU" term="%22Ferreirós-Vidal+I%22">Ferreirós-Vidal I</searchLink>; Genética de Poblaciones en Biomedicina (GenPoB) Research Group, Facultade de Medicina, Instituto de Ciencias Forenses, Instituto de Investigación Sanitaria (IDIS), Unidade de Xenética, Universidade de Santiago de Compostela, Hospital Clínico Universitario de Santiago (SERGAS), 15706, Galicia, Spain.; Genetics, Vaccines and Infections Research Group (GENVIP), Instituto de Investigación Sanitaria de Santiago, Santiago de Compostela, Galicia, Spain.; Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBER-ES), Madrid, Spain.<br /><searchLink fieldCode="AU" term="%22Salas+A%22">Salas A</searchLink>; Genética de Poblaciones en Biomedicina (GenPoB) Research Group, Facultade de Medicina, Instituto de Ciencias Forenses, Instituto de Investigación Sanitaria (IDIS), Unidade de Xenética, Universidade de Santiago de Compostela, Hospital Clínico Universitario de Santiago (SERGAS), 15706, Galicia, Spain.; Genetics, Vaccines and Infections Research Group (GENVIP), Instituto de Investigación Sanitaria de Santiago, Santiago de Compostela, Galicia, Spain.; Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBER-ES), Madrid, Spain.<br /><searchLink fieldCode="AU" term="%22Dios+S%22">Dios S</searchLink>; Instituto de Investigaciones Marinas (IIM), CSIC, Vigo, Spain.<br /><searchLink fieldCode="AU" term="%22Gambón+F%22">Gambón F</searchLink>; Department of Immunology, Vigo University Hospital Complex, Vigo, Spain.<br /><searchLink fieldCode="AU" term="%22Gestal+C%22">Gestal C</searchLink>; Instituto de Investigaciones Marinas (IIM), CSIC, Vigo, Spain. cgestal@iim.csic.es.
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