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Utilization of a CRISPRi-based ex vivo challenge model to reveal temporally dependent gene essentiality in intracellular Mycobacterium tuberculosis.

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Title: Utilization of a CRISPRi-based ex vivo challenge model to reveal temporally dependent gene essentiality in intracellular Mycobacterium tuberculosis.
Authors: Theriault ME; Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA., Wong AI; Laboratory of Host-Pathogen Biology, The Rockefeller University, New York, New York, USA., DeJesus MA; Laboratory of Host-Pathogen Biology, The Rockefeller University, New York, New York, USA., Pisu D; Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA., Nae Rin Lee B; Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA., Kirukubar G; Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA., Li S; Laboratory of Host-Pathogen Biology, The Rockefeller University, New York, New York, USA., Wallach JB; Department of Microbiology and Immunology, Weill Cornell Medicine, New York, New York, USA., Schnappinger D; Department of Microbiology and Immunology, Weill Cornell Medicine, New York, New York, USA., Lê-Bury G; Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA., Russell DG; Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA., Rock JM; Laboratory of Host-Pathogen Biology, The Rockefeller University, New York, New York, USA.
Source: MBio [mBio] 2026 May 13; Vol. 17 (5), pp. e0061026. Date of Electronic Publication: 2026 Apr 20.
Publication Type: Journal Article
Journal Info: Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 101519231 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2150-7511 (Electronic) NLM ISO Abbreviation: mBio Subsets: MEDLINE
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  Data: <searchLink fieldCode="JN" term="%22101519231%22">MBio</searchLink> [mBio] 2026 May 13; Vol. 17 (5), pp. e0061026. <i>Date of Electronic Publication: </i>2026 Apr 20.
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