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A validated FACS-based protocol for the isolation of neuronal and non-neuronal cells from frozen adult brain, suitable for integrated gene and protein analysis.

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Title: A validated FACS-based protocol for the isolation of neuronal and non-neuronal cells from frozen adult brain, suitable for integrated gene and protein analysis.
Authors: Kolasa M; Department of Pharmacology, Maj Institute of Pharmacology, Polish Academy of Sciences, Smętna 12, Kraków, 31-343, Poland. gaska@if-pan.krakow.pl., Roman A; Laboratory of Flow Cytometry, Maj Institute of Pharmacology, Polish Academy of Sciences, Smętna 12, 31-343, Kraków, Poland., Mielczarek P; Laboratory of Proteomics and Mass Spectrometry, Maj Institute of Pharmacology, Polish Academy of Sciences, Smętna 12, 31-343, Kraków, Poland.; Faculty of Materials Science and Ceramics, AGH University of Science and Technology, A. Mickiewicz Ave. 30, Kraków, 30-059, Poland., Solich J; Department of Pharmacology, Maj Institute of Pharmacology, Polish Academy of Sciences, Smętna 12, Kraków, 31-343, Poland., Faron-Górecka A; Department of Pharmacology, Maj Institute of Pharmacology, Polish Academy of Sciences, Smętna 12, Kraków, 31-343, Poland. gorecka@if-pan.krakow.pl.
Source: Pharmacological reports : PR [Pharmacol Rep] 2026 Jun; Vol. 78 (3), pp. 875-890. Date of Electronic Publication: 2026 Apr 22.
Publication Type: Journal Article
Journal Info: Publisher: Springer International Publishing Country of Publication: Switzerland NLM ID: 101234999 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2299-5684 (Electronic) Linking ISSN: 17341140 NLM ISO Abbreviation: Pharmacol Rep Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: A validated FACS-based protocol for the isolation of neuronal and non-neuronal cells from frozen adult brain, suitable for integrated gene and protein analysis.
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  Data: <searchLink fieldCode="AU" term="%22Kolasa+M%22">Kolasa M</searchLink>; Department of Pharmacology, Maj Institute of Pharmacology, Polish Academy of Sciences, Smętna 12, Kraków, 31-343, Poland. gaska@if-pan.krakow.pl.<br /><searchLink fieldCode="AU" term="%22Roman+A%22">Roman A</searchLink>; Laboratory of Flow Cytometry, Maj Institute of Pharmacology, Polish Academy of Sciences, Smętna 12, 31-343, Kraków, Poland.<br /><searchLink fieldCode="AU" term="%22Mielczarek+P%22">Mielczarek P</searchLink>; Laboratory of Proteomics and Mass Spectrometry, Maj Institute of Pharmacology, Polish Academy of Sciences, Smętna 12, 31-343, Kraków, Poland.; Faculty of Materials Science and Ceramics, AGH University of Science and Technology, A. Mickiewicz Ave. 30, Kraków, 30-059, Poland.<br /><searchLink fieldCode="AU" term="%22Solich+J%22">Solich J</searchLink>; Department of Pharmacology, Maj Institute of Pharmacology, Polish Academy of Sciences, Smętna 12, Kraków, 31-343, Poland.<br /><searchLink fieldCode="AU" term="%22Faron-Górecka+A%22">Faron-Górecka A</searchLink>; Department of Pharmacology, Maj Institute of Pharmacology, Polish Academy of Sciences, Smętna 12, Kraków, 31-343, Poland. gorecka@if-pan.krakow.pl.
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  Data: <searchLink fieldCode="JN" term="%22101234999%22">Pharmacological reports : PR</searchLink> [Pharmacol Rep] 2026 Jun; Vol. 78 (3), pp. 875-890. <i>Date of Electronic Publication: </i>2026 Apr 22.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Springer+International+Publishing%22">Springer International Publishing </searchLink><i>Country of Publication: </i>Switzerland <i>NLM ID: </i>101234999 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>2299-5684 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2217341140%22">17341140 </searchLink><i>NLM ISO Abbreviation: </i>Pharmacol Rep <i>Subsets: </i>MEDLINE
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        Value: 10.1007/s43440-026-00854-6
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              Text: 2026 Jun
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