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Technical and biological sources of noise confound multiplexed enhancer AAV screening.

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Title: Technical and biological sources of noise confound multiplexed enhancer AAV screening.
Authors: Hunker AC; Allen Institute for Brain Science, Seattle, WA, USA. avery.hunker@alleninstitute.org., Mich JK; Allen Institute for Brain Science, Seattle, WA, USA. johnmi@alleninstitute.org., Taskin N; Allen Institute for Brain Science, Seattle, WA, USA., Torkelson A; Allen Institute for Brain Science, Seattle, WA, USA., Cardenas T; Allen Institute for Brain Science, Seattle, WA, USA., Lalanne JB; Department of Genome Sciences, University of Washington, Seattle, WA, USA., Mahoney JT; Allen Institute for Brain Science, Seattle, WA, USA., Bertagnolli D; Allen Institute for Brain Science, Seattle, WA, USA., Chakka AB; Allen Institute for Brain Science, Seattle, WA, USA., Chakrabarty R; Allen Institute for Brain Science, Seattle, WA, USA., Donadio NP; Allen Institute for Brain Science, Seattle, WA, USA., Ferrer R; Allen Institute for Brain Science, Seattle, WA, USA., Gasperini M; Seattle Hub for Synthetic Biology, Seattle, WA, USA., Goldy J; Allen Institute for Brain Science, Seattle, WA, USA., Guzman J; Allen Institute for Brain Science, Seattle, WA, USA., Jin K; Allen Institute for Brain Science, Seattle, WA, USA., Khem S; Allen Institute for Brain Science, Seattle, WA, USA., Kutsal R; Allen Institute for Brain Science, Seattle, WA, USA., Martinez RA; Allen Institute for Brain Science, Seattle, WA, USA., Newman D; Allen Institute for Brain Science, Seattle, WA, USA., Pena N; Allen Institute for Brain Science, Seattle, WA, USA., Rimorin C; Allen Institute for Brain Science, Seattle, WA, USA., Rocha D; Allen Institute for Brain Science, Seattle, WA, USA., Shapovalova NV; Allen Institute for Brain Science, Seattle, WA, USA., Tieu M; Allen Institute for Brain Science, Seattle, WA, USA., Weed N; Allen Institute for Brain Science, Seattle, WA, USA., Zhou T; Allen Institute for Brain Science, Seattle, WA, USA., Hodge R; Allen Institute for Brain Science, Seattle, WA, USA., Yao S; Allen Institute for Brain Science, Seattle, WA, USA., Shendure J; Department of Genome Sciences, University of Washington, Seattle, WA, USA.; Seattle Hub for Synthetic Biology, Seattle, WA, USA.; Brotman Baty Institute for Precision Medicine, Seattle, WA, USA.; Howard Hughes Medical Institute, Seattle, WA, USA.; Allen Discovery Center for Cell Lineage Tracing, Seattle, WA, USA., Smith KA; Allen Institute for Brain Science, Seattle, WA, USA., Lein ES; Allen Institute for Brain Science, Seattle, WA, USA.; Department of Neurological Surgery, University of Washington, Seattle, WA, USA., Tasic B; Allen Institute for Brain Science, Seattle, WA, USA., Levi BP; Allen Institute for Brain Science, Seattle, WA, USA., Ting JT; Allen Institute for Brain Science, Seattle, WA, USA.; Department of Neurobiology & Biophysics, University of Washington, Seattle, WA, USA.; Washington National Biomedical Research Center, Seattle, WA, USA.
Source: Nature communications [Nat Commun] 2026 Apr 28; Vol. 17 (1). Date of Electronic Publication: 2026 Apr 28.
Publication Type: Journal Article
Journal Info: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
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  Data: <searchLink fieldCode="AU" term="%22Hunker+AC%22">Hunker AC</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA. avery.hunker@alleninstitute.org.<br /><searchLink fieldCode="AU" term="%22Mich+JK%22">Mich JK</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA. johnmi@alleninstitute.org.<br /><searchLink fieldCode="AU" term="%22Taskin+N%22">Taskin N</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Torkelson+A%22">Torkelson A</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Cardenas+T%22">Cardenas T</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Lalanne+JB%22">Lalanne JB</searchLink>; Department of Genome Sciences, University of Washington, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Mahoney+JT%22">Mahoney JT</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Bertagnolli+D%22">Bertagnolli D</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Chakka+AB%22">Chakka AB</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Chakrabarty+R%22">Chakrabarty R</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Donadio+NP%22">Donadio NP</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Ferrer+R%22">Ferrer R</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Gasperini+M%22">Gasperini M</searchLink>; Seattle Hub for Synthetic Biology, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Goldy+J%22">Goldy J</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Guzman+J%22">Guzman J</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Jin+K%22">Jin K</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Khem+S%22">Khem S</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Kutsal+R%22">Kutsal R</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Martinez+RA%22">Martinez RA</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Newman+D%22">Newman D</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Pena+N%22">Pena N</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Rimorin+C%22">Rimorin C</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Rocha+D%22">Rocha D</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Shapovalova+NV%22">Shapovalova NV</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Tieu+M%22">Tieu M</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Weed+N%22">Weed N</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Zhou+T%22">Zhou T</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Hodge+R%22">Hodge R</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Yao+S%22">Yao S</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Shendure+J%22">Shendure J</searchLink>; Department of Genome Sciences, University of Washington, Seattle, WA, USA.; Seattle Hub for Synthetic Biology, Seattle, WA, USA.; Brotman Baty Institute for Precision Medicine, Seattle, WA, USA.; Howard Hughes Medical Institute, Seattle, WA, USA.; Allen Discovery Center for Cell Lineage Tracing, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Smith+KA%22">Smith KA</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Lein+ES%22">Lein ES</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.; Department of Neurological Surgery, University of Washington, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Tasic+B%22">Tasic B</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Levi+BP%22">Levi BP</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.<br /><searchLink fieldCode="AU" term="%22Ting+JT%22">Ting JT</searchLink>; Allen Institute for Brain Science, Seattle, WA, USA.; Department of Neurobiology & Biophysics, University of Washington, Seattle, WA, USA.; Washington National Biomedical Research Center, Seattle, WA, USA.
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  Data: <searchLink fieldCode="JN" term="%22101528555%22">Nature communications</searchLink> [Nat Commun] 2026 Apr 28; Vol. 17 (1). <i>Date of Electronic Publication: </i>2026 Apr 28.
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