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STRAIGHT-IN Dual: a platform for dual single-copy integrations of DNA payloads and gene circuits into human induced pluripotent stem cells.

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Title: STRAIGHT-IN Dual: a platform for dual single-copy integrations of DNA payloads and gene circuits into human induced pluripotent stem cells.
Authors: Blanch-Asensio A; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.; The Novo Nordisk Foundation Center for Stem Cell Medicine, reNEW, Leiden University Medical Center, Leiden, The Netherlands.; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Ploessl DS; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Johnson BB; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.; The Novo Nordisk Foundation Center for Stem Cell Medicine, reNEW, Leiden University Medical Center, Leiden, The Netherlands., Cascione S; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands., Berndsen MRM; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.; The Novo Nordisk Foundation Center for Stem Cell Medicine, reNEW, Leiden University Medical Center, Leiden, The Netherlands., Wang NB; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Orlova VV; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.; The Novo Nordisk Foundation Center for Stem Cell Medicine, reNEW, Leiden University Medical Center, Leiden, The Netherlands., Alemany A; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.; The Novo Nordisk Foundation Center for Stem Cell Medicine, reNEW, Leiden University Medical Center, Leiden, The Netherlands., Mummery CL; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.; The Novo Nordisk Foundation Center for Stem Cell Medicine, reNEW, Leiden University Medical Center, Leiden, The Netherlands., Galloway KE; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA. katiegal@mit.edu., Davis RP; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands. r.p.davis@lumc.nl.; The Novo Nordisk Foundation Center for Stem Cell Medicine, reNEW, Leiden University Medical Center, Leiden, The Netherlands. r.p.davis@lumc.nl.
Source: Nature biomedical engineering [Nat Biomed Eng] 2026 Apr 30. Date of Electronic Publication: 2026 Apr 30.
Publication Type: Journal Article
Journal Info: Publisher: Springer Nature Country of Publication: England NLM ID: 101696896 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2157-846X (Electronic) Linking ISSN: 2157846X NLM ISO Abbreviation: Nat Biomed Eng Subsets: MEDLINE
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  Data: STRAIGHT-IN Dual: a platform for dual single-copy integrations of DNA payloads and gene circuits into human induced pluripotent stem cells.
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  Data: <searchLink fieldCode="AU" term="%22Blanch-Asensio+A%22">Blanch-Asensio A</searchLink>; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.; The Novo Nordisk Foundation Center for Stem Cell Medicine, reNEW, Leiden University Medical Center, Leiden, The Netherlands.; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.<br /><searchLink fieldCode="AU" term="%22Ploessl+DS%22">Ploessl DS</searchLink>; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.<br /><searchLink fieldCode="AU" term="%22Johnson+BB%22">Johnson BB</searchLink>; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.; The Novo Nordisk Foundation Center for Stem Cell Medicine, reNEW, Leiden University Medical Center, Leiden, The Netherlands.<br /><searchLink fieldCode="AU" term="%22Cascione+S%22">Cascione S</searchLink>; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.<br /><searchLink fieldCode="AU" term="%22Berndsen+MRM%22">Berndsen MRM</searchLink>; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.; The Novo Nordisk Foundation Center for Stem Cell Medicine, reNEW, Leiden University Medical Center, Leiden, The Netherlands.<br /><searchLink fieldCode="AU" term="%22Wang+NB%22">Wang NB</searchLink>; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.<br /><searchLink fieldCode="AU" term="%22Orlova+VV%22">Orlova VV</searchLink>; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.; The Novo Nordisk Foundation Center for Stem Cell Medicine, reNEW, Leiden University Medical Center, Leiden, The Netherlands.<br /><searchLink fieldCode="AU" term="%22Alemany+A%22">Alemany A</searchLink>; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.; The Novo Nordisk Foundation Center for Stem Cell Medicine, reNEW, Leiden University Medical Center, Leiden, The Netherlands.<br /><searchLink fieldCode="AU" term="%22Mummery+CL%22">Mummery CL</searchLink>; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.; The Novo Nordisk Foundation Center for Stem Cell Medicine, reNEW, Leiden University Medical Center, Leiden, The Netherlands.<br /><searchLink fieldCode="AU" term="%22Galloway+KE%22">Galloway KE</searchLink>; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA. katiegal@mit.edu.<br /><searchLink fieldCode="AU" term="%22Davis+RP%22">Davis RP</searchLink>; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands. r.p.davis@lumc.nl.; The Novo Nordisk Foundation Center for Stem Cell Medicine, reNEW, Leiden University Medical Center, Leiden, The Netherlands. r.p.davis@lumc.nl.
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