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The elephant (bird) in the room: unknown mechanisms and unresolved impacts of low DNA yields in avian microbiome research.

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Title: The elephant (bird) in the room: unknown mechanisms and unresolved impacts of low DNA yields in avian microbiome research.
Authors: Kohl KD; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, USA. kkohl@pitt.edu., Anderson R; Department of Biological Sciences, Florida Atlantic University, Davie, FL, 33314, USA., Andreadis C; Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, USA., Baiz MD; Department of Biological Sciences, University at Buffalo, SUNY, Buffalo, NY, 14260, USA., Bodawatta KH; Globe Institute, University of Copenhagen, Copenhagen, Denmark., Campos-Cerda F; Departamento de Botánica, Instituto de Biología, Universidad Nacional Autónoma de México, Ciudad de México, 04510, México.; Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad de México, 04510, México., Chaves JA; Department of Biology, San Francisco State University, San Francisco, CA, 94132, USA., Clark AB; Department Biological Sciences, Binghamton University, Binghamton, NY, USA., Costantini MS; Illinois Natural History Survey, Prairie Research Institute, University of Illinois Urbana-Champaign, Champaign, IL, USA., Davidson GL; School of Biological Sciences, University of East Anglia, Norwich, UK., Davies CS; Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä, FI-40014, Finland., Dietz MW; Groningen Institute for Evolutionary Life Sciences (GELIFES), University of Groningen, Groningen, 9747AG, The Netherlands., Florkowski M; Ecology and Evolutionary Interdisciplinary Degree Program, Texas A&M University, College Station, TX, USA., Hird SM; Molecular and Cell Biology Department, University of Connecticut, Storrs, CT, 06269, USA., Houtz JL; Department of Biology, Allegheny College, Meadville, PA, 16335, USA., Karasov WH; Department of Forest & Wildlife Ecology, University of Wisconsin-Madison, Madison, USA., Knutie SA; Department of Ecology and Evolutionary Biology, University of Connecticut, Storrs, CT, 06269, USA., Lim HC; Department of Biology, George Mason University, Fairfax, VA, USA., Mason NA; Department of Biological Science and Museum of Natural Sciences, Louisiana State University, Baton Rouge, USA., Rowe M; Department of Animal Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, 6700 AB, The Netherlands., Rudzki EN; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, USA., San Juan PA; Department of Mammalogy, Research and Collections, Natural History Museum of Los Angeles County, Los Angeles, CA, 90007, USA., Skeen HR; Negaunee Integrative Research Center, Field Museum of Natural History, Chicago, IL, 60605, USA.; Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ, 08544, USA., Slevin MC; Department of Biological Sciences, Florida Atlantic University, Davie, FL, 33314, USA., Somers S; School of Biological, Earth and Environmental Sciences, Distillery Fields, University College Cork, Cork, T23 TK30, Ireland., West AG; School of Biological Sciences, University of Auckland, Auckland, New Zealand., Worsley SF; School of Biological Sciences, University of East Anglia, Norwich, UK., Videvall E; Department of Ecology and Genetics, Uppsala University, Uppsala, 75236, Sweden., Trevelline BK; Department of Biological Sciences, Kent State University, Kent, 44242, USA.
Source: Animal microbiome [Anim Microbiome] 2026 May 18; Vol. 8 (1). Date of Electronic Publication: 2026 May 18.
Publication Type: Letter
Journal Info: Publisher: BioMed Central Country of Publication: England NLM ID: 101759457 Publication Model: Electronic Cited Medium: Internet ISSN: 2524-4671 (Electronic) Linking ISSN: 25244671 NLM ISO Abbreviation: Anim Microbiome Subsets: PubMed not MEDLINE
Database: MEDLINE Ultimate
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  Data: <searchLink fieldCode="AU" term="%22Kohl+KD%22">Kohl KD</searchLink>; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, USA. kkohl@pitt.edu.<br /><searchLink fieldCode="AU" term="%22Anderson+R%22">Anderson R</searchLink>; Department of Biological Sciences, Florida Atlantic University, Davie, FL, 33314, USA.<br /><searchLink fieldCode="AU" term="%22Andreadis+C%22">Andreadis C</searchLink>; Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, USA.<br /><searchLink fieldCode="AU" term="%22Baiz+MD%22">Baiz MD</searchLink>; Department of Biological Sciences, University at Buffalo, SUNY, Buffalo, NY, 14260, USA.<br /><searchLink fieldCode="AU" term="%22Bodawatta+KH%22">Bodawatta KH</searchLink>; Globe Institute, University of Copenhagen, Copenhagen, Denmark.<br /><searchLink fieldCode="AU" term="%22Campos-Cerda+F%22">Campos-Cerda F</searchLink>; Departamento de Botánica, Instituto de Biología, Universidad Nacional Autónoma de México, Ciudad de México, 04510, México.; Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad de México, 04510, México.<br /><searchLink fieldCode="AU" term="%22Chaves+JA%22">Chaves JA</searchLink>; Department of Biology, San Francisco State University, San Francisco, CA, 94132, USA.<br /><searchLink fieldCode="AU" term="%22Clark+AB%22">Clark AB</searchLink>; Department Biological Sciences, Binghamton University, Binghamton, NY, USA.<br /><searchLink fieldCode="AU" term="%22Costantini+MS%22">Costantini MS</searchLink>; Illinois Natural History Survey, Prairie Research Institute, University of Illinois Urbana-Champaign, Champaign, IL, USA.<br /><searchLink fieldCode="AU" term="%22Davidson+GL%22">Davidson GL</searchLink>; School of Biological Sciences, University of East Anglia, Norwich, UK.<br /><searchLink fieldCode="AU" term="%22Davies+CS%22">Davies CS</searchLink>; Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä, FI-40014, Finland.<br /><searchLink fieldCode="AU" term="%22Dietz+MW%22">Dietz MW</searchLink>; Groningen Institute for Evolutionary Life Sciences (GELIFES), University of Groningen, Groningen, 9747AG, The Netherlands.<br /><searchLink fieldCode="AU" term="%22Florkowski+M%22">Florkowski M</searchLink>; Ecology and Evolutionary Interdisciplinary Degree Program, Texas A&M University, College Station, TX, USA.<br /><searchLink fieldCode="AU" term="%22Hird+SM%22">Hird SM</searchLink>; Molecular and Cell Biology Department, University of Connecticut, Storrs, CT, 06269, USA.<br /><searchLink fieldCode="AU" term="%22Houtz+JL%22">Houtz JL</searchLink>; Department of Biology, Allegheny College, Meadville, PA, 16335, USA.<br /><searchLink fieldCode="AU" term="%22Karasov+WH%22">Karasov WH</searchLink>; Department of Forest & Wildlife Ecology, University of Wisconsin-Madison, Madison, USA.<br /><searchLink fieldCode="AU" term="%22Knutie+SA%22">Knutie SA</searchLink>; Department of Ecology and Evolutionary Biology, University of Connecticut, Storrs, CT, 06269, USA.<br /><searchLink fieldCode="AU" term="%22Lim+HC%22">Lim HC</searchLink>; Department of Biology, George Mason University, Fairfax, VA, USA.<br /><searchLink fieldCode="AU" term="%22Mason+NA%22">Mason NA</searchLink>; Department of Biological Science and Museum of Natural Sciences, Louisiana State University, Baton Rouge, USA.<br /><searchLink fieldCode="AU" term="%22Rowe+M%22">Rowe M</searchLink>; Department of Animal Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, 6700 AB, The Netherlands.<br /><searchLink fieldCode="AU" term="%22Rudzki+EN%22">Rudzki EN</searchLink>; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, USA.<br /><searchLink fieldCode="AU" term="%22San+Juan+PA%22">San Juan PA</searchLink>; Department of Mammalogy, Research and Collections, Natural History Museum of Los Angeles County, Los Angeles, CA, 90007, USA.<br /><searchLink fieldCode="AU" term="%22Skeen+HR%22">Skeen HR</searchLink>; Negaunee Integrative Research Center, Field Museum of Natural History, Chicago, IL, 60605, USA.; Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ, 08544, USA.<br /><searchLink fieldCode="AU" term="%22Slevin+MC%22">Slevin MC</searchLink>; Department of Biological Sciences, Florida Atlantic University, Davie, FL, 33314, USA.<br /><searchLink fieldCode="AU" term="%22Somers+S%22">Somers S</searchLink>; School of Biological, Earth and Environmental Sciences, Distillery Fields, University College Cork, Cork, T23 TK30, Ireland.<br /><searchLink fieldCode="AU" term="%22West+AG%22">West AG</searchLink>; School of Biological Sciences, University of Auckland, Auckland, New Zealand.<br /><searchLink fieldCode="AU" term="%22Worsley+SF%22">Worsley SF</searchLink>; School of Biological Sciences, University of East Anglia, Norwich, UK.<br /><searchLink fieldCode="AU" term="%22Videvall+E%22">Videvall E</searchLink>; Department of Ecology and Genetics, Uppsala University, Uppsala, 75236, Sweden.<br /><searchLink fieldCode="AU" term="%22Trevelline+BK%22">Trevelline BK</searchLink>; Department of Biological Sciences, Kent State University, Kent, 44242, USA.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22BioMed+Central%22">BioMed Central </searchLink><i>Country of Publication: </i>England <i>NLM ID: </i>101759457 <i>Publication Model: </i>Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>2524-4671 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2225244671%22">25244671 </searchLink><i>NLM ISO Abbreviation: </i>Anim Microbiome <i>Subsets: </i>PubMed not MEDLINE
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