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Evaluation of DNA extraction methods from clinical Mycobacterium tuberculosis primary liquid culture for whole-genome sequencing.

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Title: Evaluation of DNA extraction methods from clinical Mycobacterium tuberculosis primary liquid culture for whole-genome sequencing.
Authors: Conceição EC; South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa. emilyncosta@sun.ac.za.; Centre for Epidemic Response and Innovation (CERI), School of Data Science and Computational Thinking, Stellenbosch University, Cape Town, South Africa. emilyncosta@sun.ac.za., Haffejee M; South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa., Wells F; South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa., Mann B; South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa., Heupink T; Tuberculosis Omics Research Consortium, Department of Family Medicine and Population Health, Global Health Institute, Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium., Paulse A; South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa., Williams J; South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa., Dippenaar A; Tuberculosis Omics Research Consortium, Department of Family Medicine and Population Health, Global Health Institute, Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium., Ghebrekristos Y; National Health Laboratory Service Green Point, Cape Town, South Africa., Burger M; National Health Laboratory Service Green Point, Cape Town, South Africa., Rennie V; Tuberculosis Omics Research Consortium, Department of Family Medicine and Population Health, Global Health Institute, Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium., de Diego Fuertes M; Tuberculosis Omics Research Consortium, Department of Family Medicine and Population Health, Global Health Institute, Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium., Al-Talib I; South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa., Ismail N; South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa., Grobbelaar M; South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa., Streicher EM; South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa., de Oliveira T; Centre for Epidemic Response and Innovation (CERI), School of Data Science and Computational Thinking, Stellenbosch University, Cape Town, South Africa., Van der Spuy G; South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa., Van Rie A; Tuberculosis Omics Research Consortium, Department of Family Medicine and Population Health, Global Health Institute, Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium. annelies.vanrie@uantwerpen.be., Warren R; South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa. rw1@sun.ac.za.
Source: BMC genomics [BMC Genomics] 2026 May 19; Vol. 27 (1). Date of Electronic Publication: 2026 May 19.
Publication Type: Journal Article
Journal Info: Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2164 (Electronic) Linking ISSN: 14712164 NLM ISO Abbreviation: BMC Genomics Subsets: MEDLINE
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  Data: Evaluation of DNA extraction methods from clinical Mycobacterium tuberculosis primary liquid culture for whole-genome sequencing.
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  Data: <searchLink fieldCode="AU" term="%22Conceição+EC%22">Conceição EC</searchLink>; South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa. emilyncosta@sun.ac.za.; Centre for Epidemic Response and Innovation (CERI), School of Data Science and Computational Thinking, Stellenbosch University, Cape Town, South Africa. emilyncosta@sun.ac.za.<br /><searchLink fieldCode="AU" term="%22Haffejee+M%22">Haffejee M</searchLink>; South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.<br /><searchLink fieldCode="AU" term="%22Wells+F%22">Wells F</searchLink>; South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.<br /><searchLink fieldCode="AU" term="%22Mann+B%22">Mann B</searchLink>; South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.<br /><searchLink fieldCode="AU" term="%22Heupink+T%22">Heupink T</searchLink>; Tuberculosis Omics Research Consortium, Department of Family Medicine and Population Health, Global Health Institute, Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium.<br /><searchLink fieldCode="AU" term="%22Paulse+A%22">Paulse A</searchLink>; South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.<br /><searchLink fieldCode="AU" term="%22Williams+J%22">Williams J</searchLink>; South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.<br /><searchLink fieldCode="AU" term="%22Dippenaar+A%22">Dippenaar A</searchLink>; Tuberculosis Omics Research Consortium, Department of Family Medicine and Population Health, Global Health Institute, Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium.<br /><searchLink fieldCode="AU" term="%22Ghebrekristos+Y%22">Ghebrekristos Y</searchLink>; National Health Laboratory Service Green Point, Cape Town, South Africa.<br /><searchLink fieldCode="AU" term="%22Burger+M%22">Burger M</searchLink>; National Health Laboratory Service Green Point, Cape Town, South Africa.<br /><searchLink fieldCode="AU" term="%22Rennie+V%22">Rennie V</searchLink>; Tuberculosis Omics Research Consortium, Department of Family Medicine and Population Health, Global Health Institute, Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium.<br /><searchLink fieldCode="AU" term="%22de+Diego+Fuertes+M%22">de Diego Fuertes M</searchLink>; Tuberculosis Omics Research Consortium, Department of Family Medicine and Population Health, Global Health Institute, Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium.<br /><searchLink fieldCode="AU" term="%22Al-Talib+I%22">Al-Talib I</searchLink>; South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.<br /><searchLink fieldCode="AU" term="%22Ismail+N%22">Ismail N</searchLink>; South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.<br /><searchLink fieldCode="AU" term="%22Grobbelaar+M%22">Grobbelaar M</searchLink>; South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.<br /><searchLink fieldCode="AU" term="%22Streicher+EM%22">Streicher EM</searchLink>; South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.<br /><searchLink fieldCode="AU" term="%22de+Oliveira+T%22">de Oliveira T</searchLink>; Centre for Epidemic Response and Innovation (CERI), School of Data Science and Computational Thinking, Stellenbosch University, Cape Town, South Africa.<br /><searchLink fieldCode="AU" term="%22Van+der+Spuy+G%22">Van der Spuy G</searchLink>; South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.<br /><searchLink fieldCode="AU" term="%22Van+Rie+A%22">Van Rie A</searchLink>; Tuberculosis Omics Research Consortium, Department of Family Medicine and Population Health, Global Health Institute, Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium. annelies.vanrie@uantwerpen.be.<br /><searchLink fieldCode="AU" term="%22Warren+R%22">Warren R</searchLink>; South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa. rw1@sun.ac.za.
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