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A combined PIE-FRET and FCS assay to monitor RNA dynamics and cleavage by SARS-CoV-2 Nsp15.

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Title: A combined PIE-FRET and FCS assay to monitor RNA dynamics and cleavage by SARS-CoV-2 Nsp15.
Authors: Gordon K; Department of Physics and Astronomy, North Carolina State University, Raleigh, NC, 27695-8202, USA., Wright ZM; Molecular and Cellular Biology Laboratory, Department of Health and Human Services, National Institute of Environmental Health Sciences, National Institutes of Health, 111 T. W. Alexander Drive, Research Triangle Park, NC, 27709, USA., Stephens CR; Department of Physics and Astronomy, North Carolina State University, Raleigh, NC, 27695-8202, USA., Frazier MN; Molecular and Cellular Biology Laboratory, Department of Health and Human Services, National Institute of Environmental Health Sciences, National Institutes of Health, 111 T. W. Alexander Drive, Research Triangle Park, NC, 27709, USA.; Department of Chemistry and Biochemistry, College of Charleston, 66 George St., Charleston, SC, 29424, USA., Clark BS; Department of Physics and Astronomy, North Carolina State University, Raleigh, NC, 27695-8202, USA., Wilson IM; Molecular and Cellular Biology Laboratory, Department of Health and Human Services, National Institute of Environmental Health Sciences, National Institutes of Health, 111 T. W. Alexander Drive, Research Triangle Park, NC, 27709, USA.; Howard University College of Medicine, Washington, DC, 20059, USA., Silvernail I; Department of Physics and Astronomy, North Carolina State University, Raleigh, NC, 27695-8202, USA., Stanley RE; Molecular and Cellular Biology Laboratory, Department of Health and Human Services, National Institute of Environmental Health Sciences, National Institutes of Health, 111 T. W. Alexander Drive, Research Triangle Park, NC, 27709, USA., LeBlanc SJ; Department of Physics and Astronomy, North Carolina State University, Raleigh, NC, 27695-8202, USA. sleblan@ncsu.edu.
Source: Scientific reports [Sci Rep] 2026 May 29. Date of Electronic Publication: 2026 May 29.
Publication Type: Journal Article
Journal Info: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
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