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Effect of ultrasonic irrigant activation and negative apical pressure in removing mature biofilm from the isthmus of curved root canal: A 3D-printed model study.

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Title: Effect of ultrasonic irrigant activation and negative apical pressure in removing mature biofilm from the isthmus of curved root canal: A 3D-printed model study.
Authors: de Maceda FG; Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil. Electronic address: flaviodemaceda@usp.br., Lopes-Olhê FC; Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil. Electronic address: fabiane.lopes@usp.br., Torres-Carrillo AJS; Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil. Electronic address: angelo.tc@usp.br., Goulart RS; School of Dentistry, University of Ribeirão Preto (UNAERP), Ribeirão Preto, SP, Brazil. Electronic address: rafael.goulart@sou.unaerp.edu.br., Petean IBF; Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil. Electronic address: igor.petean@usp.br., de Castro-Vasconcelos GA; Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil. Electronic address: gustavo98acv@usp.br., Silva-Sousa AC; Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil. Electronic address: alicesousa@usp.br., Estrela C; School of Dentistry, Federal University of Goiás, Goiânia, GO, Brazil., Mazzi-Chaves JF; Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil. Electronic address: jardel.chaves@usp.br., Silva-Sousa YTC; School of Dentistry, University of Ribeirão Preto (UNAERP), Ribeirão Preto, SP, Brazil. Electronic address: ysousa@unaerp.br., Pitondo-Silva A; School of Dentistry, University of Ribeirão Preto (UNAERP), Ribeirão Preto, SP, Brazil. Electronic address: andre@pitondo.com.br., Sousa-Neto MD; Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil. Electronic address: sousanet@forp.usp.br.
Source: Journal of microbiological methods [J Microbiol Methods] 2026 Aug; Vol. 247, pp. 107567. Date of Electronic Publication: 2026 Jun 01.
Publication Type: Journal Article
Journal Info: Publisher: Elsevier Biomedical Country of Publication: Netherlands NLM ID: 8306883 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-8359 (Electronic) Linking ISSN: 01677012 NLM ISO Abbreviation: J Microbiol Methods Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: Effect of ultrasonic irrigant activation and negative apical pressure in removing mature biofilm from the isthmus of curved root canal: A 3D-printed model study.
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  Data: <searchLink fieldCode="AU" term="%22de+Maceda+FG%22">de Maceda FG</searchLink>; Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil. Electronic address: flaviodemaceda@usp.br.<br /><searchLink fieldCode="AU" term="%22Lopes-Olhê+FC%22">Lopes-Olhê FC</searchLink>; Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil. Electronic address: fabiane.lopes@usp.br.<br /><searchLink fieldCode="AU" term="%22Torres-Carrillo+AJS%22">Torres-Carrillo AJS</searchLink>; Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil. Electronic address: angelo.tc@usp.br.<br /><searchLink fieldCode="AU" term="%22Goulart+RS%22">Goulart RS</searchLink>; School of Dentistry, University of Ribeirão Preto (UNAERP), Ribeirão Preto, SP, Brazil. Electronic address: rafael.goulart@sou.unaerp.edu.br.<br /><searchLink fieldCode="AU" term="%22Petean+IBF%22">Petean IBF</searchLink>; Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil. Electronic address: igor.petean@usp.br.<br /><searchLink fieldCode="AU" term="%22de+Castro-Vasconcelos+GA%22">de Castro-Vasconcelos GA</searchLink>; Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil. Electronic address: gustavo98acv@usp.br.<br /><searchLink fieldCode="AU" term="%22Silva-Sousa+AC%22">Silva-Sousa AC</searchLink>; Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil. Electronic address: alicesousa@usp.br.<br /><searchLink fieldCode="AU" term="%22Estrela+C%22">Estrela C</searchLink>; School of Dentistry, Federal University of Goiás, Goiânia, GO, Brazil.<br /><searchLink fieldCode="AU" term="%22Mazzi-Chaves+JF%22">Mazzi-Chaves JF</searchLink>; Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil. Electronic address: jardel.chaves@usp.br.<br /><searchLink fieldCode="AU" term="%22Silva-Sousa+YTC%22">Silva-Sousa YTC</searchLink>; School of Dentistry, University of Ribeirão Preto (UNAERP), Ribeirão Preto, SP, Brazil. Electronic address: ysousa@unaerp.br.<br /><searchLink fieldCode="AU" term="%22Pitondo-Silva+A%22">Pitondo-Silva A</searchLink>; School of Dentistry, University of Ribeirão Preto (UNAERP), Ribeirão Preto, SP, Brazil. Electronic address: andre@pitondo.com.br.<br /><searchLink fieldCode="AU" term="%22Sousa-Neto+MD%22">Sousa-Neto MD</searchLink>; Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil. Electronic address: sousanet@forp.usp.br.
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  Data: <searchLink fieldCode="JN" term="%228306883%22">Journal of microbiological methods</searchLink> [J Microbiol Methods] 2026 Aug; Vol. 247, pp. 107567. <i>Date of Electronic Publication: </i>2026 Jun 01.
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