Effect of ultrasonic irrigant activation and negative apical pressure in removing mature biofilm from the isthmus of curved root canal: A 3D-printed model study. - de Maceda FG; Lopes-Olhê FC; Torres-Carrillo AJS; Goulart RS; Petean IBF; de Castro-Vasconcelos GA; Silva-Sousa AC; Estrela C; Mazzi-Chaves JF; Silva-Sousa YTC; Pitondo-Silva A; Sousa-Neto MD (Journal of microbiological methods, 2026)