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Direct inoculation of bioreactor-controlled stirred suspension culture with cryopreserved human pluripotent stem cells.

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Title: Direct inoculation of bioreactor-controlled stirred suspension culture with cryopreserved human pluripotent stem cells.
Authors: Cyrys K; Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department of Cardiothoracic Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany. Cyrys.Kevin@mh-hannover.de.; REBIRTH Research Center for Translational and Regenerative Medicine, Hannover Medical School, Hannover, Germany. Cyrys.Kevin@mh-hannover.de., Manstein F; Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department of Cardiothoracic Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany.; REBIRTH Research Center for Translational and Regenerative Medicine, Hannover Medical School, Hannover, Germany., Triebert W; Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department of Cardiothoracic Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany.; REBIRTH Research Center for Translational and Regenerative Medicine, Hannover Medical School, Hannover, Germany., Kriedemann N; Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department of Cardiothoracic Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany.; REBIRTH Research Center for Translational and Regenerative Medicine, Hannover Medical School, Hannover, Germany., Hernandez-Bautista CA; Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department of Cardiothoracic Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany.; REBIRTH Research Center for Translational and Regenerative Medicine, Hannover Medical School, Hannover, Germany., Zweigerdt R; Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department of Cardiothoracic Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany. Zweigerdt.Robert@mh-hannover.de.; REBIRTH Research Center for Translational and Regenerative Medicine, Hannover Medical School, Hannover, Germany. Zweigerdt.Robert@mh-hannover.de.
Source: Nature protocols [Nat Protoc] 2026 Jun 22. Date of Electronic Publication: 2026 Jun 22.
Publication Type: Journal Article; Review
Journal Info: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101284307 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1750-2799 (Electronic) Linking ISSN: 17502799 NLM ISO Abbreviation: Nat Protoc Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:1750-2799
DOI:10.1038/s41596-026-01398-y