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Direct inoculation of bioreactor-controlled stirred suspension culture with cryopreserved human pluripotent stem cells.

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Title: Direct inoculation of bioreactor-controlled stirred suspension culture with cryopreserved human pluripotent stem cells.
Authors: Cyrys K; Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department of Cardiothoracic Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany. Cyrys.Kevin@mh-hannover.de.; REBIRTH Research Center for Translational and Regenerative Medicine, Hannover Medical School, Hannover, Germany. Cyrys.Kevin@mh-hannover.de., Manstein F; Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department of Cardiothoracic Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany.; REBIRTH Research Center for Translational and Regenerative Medicine, Hannover Medical School, Hannover, Germany., Triebert W; Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department of Cardiothoracic Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany.; REBIRTH Research Center for Translational and Regenerative Medicine, Hannover Medical School, Hannover, Germany., Kriedemann N; Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department of Cardiothoracic Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany.; REBIRTH Research Center for Translational and Regenerative Medicine, Hannover Medical School, Hannover, Germany., Hernandez-Bautista CA; Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department of Cardiothoracic Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany.; REBIRTH Research Center for Translational and Regenerative Medicine, Hannover Medical School, Hannover, Germany., Zweigerdt R; Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department of Cardiothoracic Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany. Zweigerdt.Robert@mh-hannover.de.; REBIRTH Research Center for Translational and Regenerative Medicine, Hannover Medical School, Hannover, Germany. Zweigerdt.Robert@mh-hannover.de.
Source: Nature protocols [Nat Protoc] 2026 Jun 22. Date of Electronic Publication: 2026 Jun 22.
Publication Type: Journal Article; Review
Journal Info: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101284307 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1750-2799 (Electronic) Linking ISSN: 17502799 NLM ISO Abbreviation: Nat Protoc Subsets: MEDLINE
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  Data: Direct inoculation of bioreactor-controlled stirred suspension culture with cryopreserved human pluripotent stem cells.
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  Data: <searchLink fieldCode="AU" term="%22Cyrys+K%22">Cyrys K</searchLink>; Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department of Cardiothoracic Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany. Cyrys.Kevin@mh-hannover.de.; REBIRTH Research Center for Translational and Regenerative Medicine, Hannover Medical School, Hannover, Germany. Cyrys.Kevin@mh-hannover.de.<br /><searchLink fieldCode="AU" term="%22Manstein+F%22">Manstein F</searchLink>; Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department of Cardiothoracic Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany.; REBIRTH Research Center for Translational and Regenerative Medicine, Hannover Medical School, Hannover, Germany.<br /><searchLink fieldCode="AU" term="%22Triebert+W%22">Triebert W</searchLink>; Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department of Cardiothoracic Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany.; REBIRTH Research Center for Translational and Regenerative Medicine, Hannover Medical School, Hannover, Germany.<br /><searchLink fieldCode="AU" term="%22Kriedemann+N%22">Kriedemann N</searchLink>; Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department of Cardiothoracic Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany.; REBIRTH Research Center for Translational and Regenerative Medicine, Hannover Medical School, Hannover, Germany.<br /><searchLink fieldCode="AU" term="%22Hernandez-Bautista+CA%22">Hernandez-Bautista CA</searchLink>; Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department of Cardiothoracic Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany.; REBIRTH Research Center for Translational and Regenerative Medicine, Hannover Medical School, Hannover, Germany.<br /><searchLink fieldCode="AU" term="%22Zweigerdt+R%22">Zweigerdt R</searchLink>; Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department of Cardiothoracic Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany. Zweigerdt.Robert@mh-hannover.de.; REBIRTH Research Center for Translational and Regenerative Medicine, Hannover Medical School, Hannover, Germany. Zweigerdt.Robert@mh-hannover.de.
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  Data: <searchLink fieldCode="JN" term="%22101284307%22">Nature protocols</searchLink> [Nat Protoc] 2026 Jun 22. <i>Date of Electronic Publication: </i>2026 Jun 22.
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        Value: 10.1038/s41596-026-01398-y
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              Text: 2026 Jun 22
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