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Reply to Chen et al., "Methodological considerations for the establishment of epidemiological cutoff values for Sporothrix species using CLSI broth microdilution".

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Title: Reply to Chen et al., "Methodological considerations for the establishment of epidemiological cutoff values for Sporothrix species using CLSI broth microdilution".
Authors: Dos Santos A; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, Brazil.; Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA., Bonifaz A; Dermatology Service, Mycology Department, Hospital General de México, 'Dr. Eduardo Liceaga', Mexico City, Mexico., Bombassaro A; Department of Public Health, Federal University of Paraná, Curitiba, Brazil.; Radboudumc-CWZ Center of Expertise for Mycology, Canisius-Wilhelmina Hospital (CWZ), Nijmegen, the Netherlands., de Sá Machado AC; Fundação Oswaldo Cruz (Fiocruz), Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos (Lapclin-Dermzoo), Instituto Nacional de Infectologia Evandro Chagas (INI), Rio de Janeiro, Rio de Janeiro, Brazil., Rodrigues AM; Department of Microbiology, Immunology, and Parasitology, Discipline of Cellular Biology, Federal University of Sao Paulo (UNIFESP), São Paulo, Brazil.; National Institute of Science and Technology in Human Pathogenic Fungi, São Paulo, Brazil., Borman AM; UK Health Security Agency National Mycology Reference Laboratory, Bristol and MRC Centre for Medical Mycology, University of Exeter, Exeter, United Kingdom., Chakrabarti A; Doodhadhari Burfani Hospital and Research Institute, Haridwar, India., Chowdary A; Medical Mycology Unit, Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India., Rediguieri B; Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.; Department of Pathology, Universidade Federal do Espírito Santo (UFES), Vitória, Espírito Santo, Brazil., Johnson EM; UK Health Security Agency National Mycology Reference Laboratory, Bristol and MRC Centre for Medical Mycology, University of Exeter, Exeter, United Kingdom., Hagen F; Radboudumc-CWZ Center of Expertise for Mycology, Canisius-Wilhelmina Hospital (CWZ), Nijmegen, the Netherlands.; Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, the Netherlands., Queiroz-Telles F; Department of Public Health, Federal University of Paraná, Curitiba, Brazil., Gonzalez GM; Faculty of Medicine, Autonomous University of Nuevo León, Monterrey, Mexico., Garcia-Effron G; Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Universidad Nacional del Litoral, Santa Fe de la Vera Cruz, Santa Fe, Argentina., Dib Ferreira Gremião I; Fundação Oswaldo Cruz (Fiocruz), Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos (Lapclin-Dermzoo), Instituto Nacional de Infectologia Evandro Chagas (INI), Rio de Janeiro, Rio de Janeiro, Brazil., Meis JF; Radboudumc-CWZ Center of Expertise for Mycology, Canisius-Wilhelmina Hospital (CWZ), Nijmegen, the Netherlands.; Excellence Center for Medical Mycology (ECMM), Institute of Translational Research, University of Cologne, Cologne, Germany., Possato Fernandes Takahashi J; Centro de Patologia, Núcleo de Patologia Quantitativa, Instituto Adolfo Lutz, São Paulo, Brazil., Pereira Borba Dos Santos L; Laboratório de Biologia Celular de Fungos, Centro de Pesquisas em Medicina de Precisão, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Rio de Janeiro, Brazil., Melhem MSC; Faculdade de Medicina, Universidade Federal do Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil.; Laboratório de ainvestigação Médica, Hospital das Clinicas, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil., Cappellano P; Microbiology Section, Grupo Fleury, São Paulo, São Paulo, Brazil., Nogueira Brilhante RS; Department of Pathology and Legal Medicine, School of Medicine, One Health Microbiology Laboratory, Postgraduate Program in Medical Microbiology, Postgraduate Program in Medical Sciences, Federal University of Ceará, Fortaleza, Ceará, Brazil., Pereira SA; Fundação Oswaldo Cruz (Fiocruz), Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos (Lapclin-Dermzoo), Instituto Nacional de Infectologia Evandro Chagas (INI), Rio de Janeiro, Rio de Janeiro, Brazil., Santos Gonçalvez S; Department of Pathology, Universidade Federal do Espírito Santo (UFES), Vitória, Espírito Santo, Brazil., Kidd S; National Mycology Reference Centre, SA Pathology, Adelaide, Australia., Zhang SX; Department of Pathology, Johns Hopkins University School of Medicine & Johns Hopkins Hospital, Baltimore, Maryland, USA., Rudramurthy S; Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, Chandigarh, India., Rozental S; Laboratório de Biologia Celular de Fungos, Centro de Pesquisas em Medicina de Precisão, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Rio de Janeiro, Brazil., Cordoba S; Mycology Department of INEIA ANLIS 'Dr. C. G. Malbran', Buenos Aires, Argentina., de Groot T; Radboudumc-CWZ Center of Expertise for Mycology, Canisius-Wilhelmina Hospital (CWZ), Nijmegen, the Netherlands., Liu W; Peking University First Hospital, Beijing, Beijing, China., Wiederhold NP; Department of Pathology and Laboratory Medicine, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA., Meijer EFJ; Radboudumc-CWZ Center of Expertise for Mycology, Canisius-Wilhelmina Hospital (CWZ), Nijmegen, the Netherlands., Lockhart SR; Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA., Dufresne PJ; Laboratoire de santé publique du Québec, Institut National de Santé Publique du Québec (INSPQ), Sainte-Anne-de-Bellevue, Quebec, Canada.
Source: Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2026 Jun 24, pp. e0056626. Date of Electronic Publication: 2026 Jun 24.
Publication Type: Journal Article
Journal Info: Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 0315061 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-6596 (Electronic) Linking ISSN: 00664804 NLM ISO Abbreviation: Antimicrob Agents Chemother Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: Reply to Chen et al., "Methodological considerations for the establishment of epidemiological cutoff values for Sporothrix species using CLSI broth microdilution".
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  Data: <searchLink fieldCode="AU" term="%22Dos+Santos+A%22">Dos Santos A</searchLink>; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, Brazil.; Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.<br /><searchLink fieldCode="AU" term="%22Bonifaz+A%22">Bonifaz A</searchLink>; Dermatology Service, Mycology Department, Hospital General de México, 'Dr. Eduardo Liceaga', Mexico City, Mexico.<br /><searchLink fieldCode="AU" term="%22Bombassaro+A%22">Bombassaro A</searchLink>; Department of Public Health, Federal University of Paraná, Curitiba, Brazil.; Radboudumc-CWZ Center of Expertise for Mycology, Canisius-Wilhelmina Hospital (CWZ), Nijmegen, the Netherlands.<br /><searchLink fieldCode="AU" term="%22de+Sá+Machado+AC%22">de Sá Machado AC</searchLink>; Fundação Oswaldo Cruz (Fiocruz), Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos (Lapclin-Dermzoo), Instituto Nacional de Infectologia Evandro Chagas (INI), Rio de Janeiro, Rio de Janeiro, Brazil.<br /><searchLink fieldCode="AU" term="%22Rodrigues+AM%22">Rodrigues AM</searchLink>; Department of Microbiology, Immunology, and Parasitology, Discipline of Cellular Biology, Federal University of Sao Paulo (UNIFESP), São Paulo, Brazil.; National Institute of Science and Technology in Human Pathogenic Fungi, São Paulo, Brazil.<br /><searchLink fieldCode="AU" term="%22Borman+AM%22">Borman AM</searchLink>; UK Health Security Agency National Mycology Reference Laboratory, Bristol and MRC Centre for Medical Mycology, University of Exeter, Exeter, United Kingdom.<br /><searchLink fieldCode="AU" term="%22Chakrabarti+A%22">Chakrabarti A</searchLink>; Doodhadhari Burfani Hospital and Research Institute, Haridwar, India.<br /><searchLink fieldCode="AU" term="%22Chowdary+A%22">Chowdary A</searchLink>; Medical Mycology Unit, Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India.<br /><searchLink fieldCode="AU" term="%22Rediguieri+B%22">Rediguieri B</searchLink>; Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.; Department of Pathology, Universidade Federal do Espírito Santo (UFES), Vitória, Espírito Santo, Brazil.<br /><searchLink fieldCode="AU" term="%22Johnson+EM%22">Johnson EM</searchLink>; UK Health Security Agency National Mycology Reference Laboratory, Bristol and MRC Centre for Medical Mycology, University of Exeter, Exeter, United Kingdom.<br /><searchLink fieldCode="AU" term="%22Hagen+F%22">Hagen F</searchLink>; Radboudumc-CWZ Center of Expertise for Mycology, Canisius-Wilhelmina Hospital (CWZ), Nijmegen, the Netherlands.; Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, the Netherlands.<br /><searchLink fieldCode="AU" term="%22Queiroz-Telles+F%22">Queiroz-Telles F</searchLink>; Department of Public Health, Federal University of Paraná, Curitiba, Brazil.<br /><searchLink fieldCode="AU" term="%22Gonzalez+GM%22">Gonzalez GM</searchLink>; Faculty of Medicine, Autonomous University of Nuevo León, Monterrey, Mexico.<br /><searchLink fieldCode="AU" term="%22Garcia-Effron+G%22">Garcia-Effron G</searchLink>; Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Universidad Nacional del Litoral, Santa Fe de la Vera Cruz, Santa Fe, Argentina.<br /><searchLink fieldCode="AU" term="%22Dib+Ferreira+Gremião+I%22">Dib Ferreira Gremião I</searchLink>; Fundação Oswaldo Cruz (Fiocruz), Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos (Lapclin-Dermzoo), Instituto Nacional de Infectologia Evandro Chagas (INI), Rio de Janeiro, Rio de Janeiro, Brazil.<br /><searchLink fieldCode="AU" term="%22Meis+JF%22">Meis JF</searchLink>; Radboudumc-CWZ Center of Expertise for Mycology, Canisius-Wilhelmina Hospital (CWZ), Nijmegen, the Netherlands.; Excellence Center for Medical Mycology (ECMM), Institute of Translational Research, University of Cologne, Cologne, Germany.<br /><searchLink fieldCode="AU" term="%22Possato+Fernandes+Takahashi+J%22">Possato Fernandes Takahashi J</searchLink>; Centro de Patologia, Núcleo de Patologia Quantitativa, Instituto Adolfo Lutz, São Paulo, Brazil.<br /><searchLink fieldCode="AU" term="%22Pereira+Borba+Dos+Santos+L%22">Pereira Borba Dos Santos L</searchLink>; Laboratório de Biologia Celular de Fungos, Centro de Pesquisas em Medicina de Precisão, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Rio de Janeiro, Brazil.<br /><searchLink fieldCode="AU" term="%22Melhem+MSC%22">Melhem MSC</searchLink>; Faculdade de Medicina, Universidade Federal do Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil.; Laboratório de ainvestigação Médica, Hospital das Clinicas, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil.<br /><searchLink fieldCode="AU" term="%22Cappellano+P%22">Cappellano P</searchLink>; Microbiology Section, Grupo Fleury, São Paulo, São Paulo, Brazil.<br /><searchLink fieldCode="AU" term="%22Nogueira+Brilhante+RS%22">Nogueira Brilhante RS</searchLink>; Department of Pathology and Legal Medicine, School of Medicine, One Health Microbiology Laboratory, Postgraduate Program in Medical Microbiology, Postgraduate Program in Medical Sciences, Federal University of Ceará, Fortaleza, Ceará, Brazil.<br /><searchLink fieldCode="AU" term="%22Pereira+SA%22">Pereira SA</searchLink>; Fundação Oswaldo Cruz (Fiocruz), Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos (Lapclin-Dermzoo), Instituto Nacional de Infectologia Evandro Chagas (INI), Rio de Janeiro, Rio de Janeiro, Brazil.<br /><searchLink fieldCode="AU" term="%22Santos+Gonçalvez+S%22">Santos Gonçalvez S</searchLink>; Department of Pathology, Universidade Federal do Espírito Santo (UFES), Vitória, Espírito Santo, Brazil.<br /><searchLink fieldCode="AU" term="%22Kidd+S%22">Kidd S</searchLink>; National Mycology Reference Centre, SA Pathology, Adelaide, Australia.<br /><searchLink fieldCode="AU" term="%22Zhang+SX%22">Zhang SX</searchLink>; Department of Pathology, Johns Hopkins University School of Medicine & Johns Hopkins Hospital, Baltimore, Maryland, USA.<br /><searchLink fieldCode="AU" term="%22Rudramurthy+S%22">Rudramurthy S</searchLink>; Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, Chandigarh, India.<br /><searchLink fieldCode="AU" term="%22Rozental+S%22">Rozental S</searchLink>; Laboratório de Biologia Celular de Fungos, Centro de Pesquisas em Medicina de Precisão, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Rio de Janeiro, Brazil.<br /><searchLink fieldCode="AU" term="%22Cordoba+S%22">Cordoba S</searchLink>; Mycology Department of INEIA ANLIS 'Dr. C. G. Malbran', Buenos Aires, Argentina.<br /><searchLink fieldCode="AU" term="%22de+Groot+T%22">de Groot T</searchLink>; Radboudumc-CWZ Center of Expertise for Mycology, Canisius-Wilhelmina Hospital (CWZ), Nijmegen, the Netherlands.<br /><searchLink fieldCode="AU" term="%22Liu+W%22">Liu W</searchLink>; Peking University First Hospital, Beijing, Beijing, China.<br /><searchLink fieldCode="AU" term="%22Wiederhold+NP%22">Wiederhold NP</searchLink>; Department of Pathology and Laboratory Medicine, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA.<br /><searchLink fieldCode="AU" term="%22Meijer+EFJ%22">Meijer EFJ</searchLink>; Radboudumc-CWZ Center of Expertise for Mycology, Canisius-Wilhelmina Hospital (CWZ), Nijmegen, the Netherlands.<br /><searchLink fieldCode="AU" term="%22Lockhart+SR%22">Lockhart SR</searchLink>; Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.<br /><searchLink fieldCode="AU" term="%22Dufresne+PJ%22">Dufresne PJ</searchLink>; Laboratoire de santé publique du Québec, Institut National de Santé Publique du Québec (INSPQ), Sainte-Anne-de-Bellevue, Quebec, Canada.
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