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Double Electroporation Combined with Zona Pellucida Removal Improves Biallelic Genome Editing Efficiency in Porcine Embryos.

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Title: Double Electroporation Combined with Zona Pellucida Removal Improves Biallelic Genome Editing Efficiency in Porcine Embryos.
Authors: Torigoe N; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.; Bio-Innovation Research Center, Tokushima University, Tokushima 7793233, Japan., Otoi T; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.; Bio-Innovation Research Center, Tokushima University, Tokushima 7793233, Japan.; Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.; Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand., Wittayarat M; Faculty of Veterinary Science, Prince of Songkla University, Songkhla 90110, Thailand., Widodo OS; Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia., Tharasanit T; Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand., Chatdarong K; Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand., Nagahara M; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.; Bio-Innovation Research Center, Tokushima University, Tokushima 7793233, Japan., Hirata M; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.; Bio-Innovation Research Center, Tokushima University, Tokushima 7793233, Japan., Tanihara F; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.; Bio-Innovation Research Center, Tokushima University, Tokushima 7793233, Japan., Namula Z; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.; Bio-Innovation Research Center, Tokushima University, Tokushima 7793233, Japan.
Source: Animals : an open access journal from MDPI [Animals (Basel)] 2026 Jun 20; Vol. 16 (12). Date of Electronic Publication: 2026 Jun 20.
Publication Type: Journal Article
Journal Info: Publisher: Molecular Diversity Preservation International Country of Publication: Switzerland NLM ID: 101635614 Publication Model: Electronic Cited Medium: Print ISSN: 2076-2615 (Print) Linking ISSN: 20762615 NLM ISO Abbreviation: Animals (Basel) Subsets: PubMed not MEDLINE
Database: MEDLINE Ultimate
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  Data: Double Electroporation Combined with Zona Pellucida Removal Improves Biallelic Genome Editing Efficiency in Porcine Embryos.
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  Data: <searchLink fieldCode="AU" term="%22Torigoe+N%22">Torigoe N</searchLink>; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.; Bio-Innovation Research Center, Tokushima University, Tokushima 7793233, Japan.<br /><searchLink fieldCode="AU" term="%22Otoi+T%22">Otoi T</searchLink>; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.; Bio-Innovation Research Center, Tokushima University, Tokushima 7793233, Japan.; Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.; Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.<br /><searchLink fieldCode="AU" term="%22Wittayarat+M%22">Wittayarat M</searchLink>; Faculty of Veterinary Science, Prince of Songkla University, Songkhla 90110, Thailand.<br /><searchLink fieldCode="AU" term="%22Widodo+OS%22">Widodo OS</searchLink>; Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.<br /><searchLink fieldCode="AU" term="%22Tharasanit+T%22">Tharasanit T</searchLink>; Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.<br /><searchLink fieldCode="AU" term="%22Chatdarong+K%22">Chatdarong K</searchLink>; Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.<br /><searchLink fieldCode="AU" term="%22Nagahara+M%22">Nagahara M</searchLink>; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.; Bio-Innovation Research Center, Tokushima University, Tokushima 7793233, Japan.<br /><searchLink fieldCode="AU" term="%22Hirata+M%22">Hirata M</searchLink>; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.; Bio-Innovation Research Center, Tokushima University, Tokushima 7793233, Japan.<br /><searchLink fieldCode="AU" term="%22Tanihara+F%22">Tanihara F</searchLink>; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.; Bio-Innovation Research Center, Tokushima University, Tokushima 7793233, Japan.<br /><searchLink fieldCode="AU" term="%22Namula+Z%22">Namula Z</searchLink>; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.; Bio-Innovation Research Center, Tokushima University, Tokushima 7793233, Japan.
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  Data: <searchLink fieldCode="JN" term="%22101635614%22">Animals : an open access journal from MDPI</searchLink> [Animals (Basel)] 2026 Jun 20; Vol. 16 (12). <i>Date of Electronic Publication: </i>2026 Jun 20.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Molecular+Diversity+Preservation+International%22">Molecular Diversity Preservation International </searchLink><i>Country of Publication: </i>Switzerland <i>NLM ID: </i>101635614 <i>Publication Model: </i>Electronic <i>Cited Medium: </i>Print <i>ISSN: </i>2076-2615 (Print) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2220762615%22">20762615 </searchLink><i>NLM ISO Abbreviation: </i>Animals (Basel) <i>Subsets: </i>PubMed not MEDLINE
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        Value: 10.3390/ani16121919
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        Text: English
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      – TitleFull: Double Electroporation Combined with Zona Pellucida Removal Improves Biallelic Genome Editing Efficiency in Porcine Embryos.
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              Text: 2026 Jun 20
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