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Inducing physiological polarity and performing gene editing using CRISPR-Cas9 in human trophoblast organoids.

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Title: Inducing physiological polarity and performing gene editing using CRISPR-Cas9 in human trophoblast organoids.
Authors: Yang L; Department of Integrative Immunobiology, Duke University School of Medicine, Durham, NC, USA. henry.yang@duke.edu., Hatterschide J; Department of Integrative Immunobiology, Duke University School of Medicine, Durham, NC, USA., Worota RE; Department of Integrative Immunobiology, Duke University School of Medicine, Durham, NC, USA., Cooley K; Department of Integrative Immunobiology, Duke University School of Medicine, Durham, NC, USA., Coyne CB; Department of Integrative Immunobiology, Duke University School of Medicine, Durham, NC, USA. carolyn.coyne@duke.edu.; Duke Human Vaccine Institute, Duke University School of Medicine, Durham, NC, USA. carolyn.coyne@duke.edu.
Source: Nature protocols [Nat Protoc] 2026 Jun 29. Date of Electronic Publication: 2026 Jun 29.
Publication Type: Journal Article; Review
Journal Info: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101284307 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1750-2799 (Electronic) Linking ISSN: 17502799 NLM ISO Abbreviation: Nat Protoc Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: <searchLink fieldCode="AU" term="%22Yang+L%22">Yang L</searchLink>; Department of Integrative Immunobiology, Duke University School of Medicine, Durham, NC, USA. henry.yang@duke.edu.<br /><searchLink fieldCode="AU" term="%22Hatterschide+J%22">Hatterschide J</searchLink>; Department of Integrative Immunobiology, Duke University School of Medicine, Durham, NC, USA.<br /><searchLink fieldCode="AU" term="%22Worota+RE%22">Worota RE</searchLink>; Department of Integrative Immunobiology, Duke University School of Medicine, Durham, NC, USA.<br /><searchLink fieldCode="AU" term="%22Cooley+K%22">Cooley K</searchLink>; Department of Integrative Immunobiology, Duke University School of Medicine, Durham, NC, USA.<br /><searchLink fieldCode="AU" term="%22Coyne+CB%22">Coyne CB</searchLink>; Department of Integrative Immunobiology, Duke University School of Medicine, Durham, NC, USA. carolyn.coyne@duke.edu.; Duke Human Vaccine Institute, Duke University School of Medicine, Durham, NC, USA. carolyn.coyne@duke.edu.
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  Data: <searchLink fieldCode="JN" term="%22101284307%22">Nature protocols</searchLink> [Nat Protoc] 2026 Jun 29. <i>Date of Electronic Publication: </i>2026 Jun 29.
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        Value: 10.1038/s41596-026-01402-5
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