Development of a 3-day manufacturing method to generate CD19-CD20-CD22 trispecific CAR T-cells from whole blood.

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Title: Development of a 3-day manufacturing method to generate CD19-CD20-CD22 trispecific CAR T-cells from whole blood.
Authors: Vignola I; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Prochazkova M; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Shao L; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Fuksenko T; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Sarkar S; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Lei H; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Jin J; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Xiong Y; Caring Cross, Gaithersburg, MD, USA., Xie Y; Caring Cross, Gaithersburg, MD, USA., Oparaocha I; Caring Cross, Gaithersburg, MD, USA., Slessareva O; Caring Cross, Gaithersburg, MD, USA., Forrest M; Caring Cross, Gaithersburg, MD, USA., Orentas R; Caring Cross, Gaithersburg, MD, USA.; Department Biochemistry and Molecular Biology, The Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA., Zhu Z; Caring Cross, Gaithersburg, MD, USA., Dropulic B; Caring Cross, Gaithersburg, MD, USA., Jin P; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Somerville RP; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Stroncek DF; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Song HW; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Highfill SL; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA. Steven.Highfill@NIH.Gov.
Source: Journal of translational medicine [J Transl Med] 2026 Jul 15; Vol. 24 (1). Date of Electronic Publication: 2026 Jul 15.
Publication Type: Journal Article
Journal Info: Publisher: BioMed Central Country of Publication: England NLM ID: 101190741 Publication Model: Electronic Cited Medium: Internet ISSN: 1479-5876 (Electronic) Linking ISSN: 14795876 NLM ISO Abbreviation: J Transl Med Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:1479-5876
DOI:10.1186/s12967-026-08306-8