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Development of a 3-day manufacturing method to generate CD19-CD20-CD22 trispecific CAR T-cells from whole blood.

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Title: Development of a 3-day manufacturing method to generate CD19-CD20-CD22 trispecific CAR T-cells from whole blood.
Authors: Vignola I; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Prochazkova M; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Shao L; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Fuksenko T; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Sarkar S; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Lei H; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Jin J; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Xiong Y; Caring Cross, Gaithersburg, MD, USA., Xie Y; Caring Cross, Gaithersburg, MD, USA., Oparaocha I; Caring Cross, Gaithersburg, MD, USA., Slessareva O; Caring Cross, Gaithersburg, MD, USA., Forrest M; Caring Cross, Gaithersburg, MD, USA., Orentas R; Caring Cross, Gaithersburg, MD, USA.; Department Biochemistry and Molecular Biology, The Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA., Zhu Z; Caring Cross, Gaithersburg, MD, USA., Dropulic B; Caring Cross, Gaithersburg, MD, USA., Jin P; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Somerville RP; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Stroncek DF; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Song HW; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Highfill SL; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA. Steven.Highfill@NIH.Gov.
Source: Journal of translational medicine [J Transl Med] 2026 Jul 15; Vol. 24 (1). Date of Electronic Publication: 2026 Jul 15.
Publication Type: Journal Article
Journal Info: Publisher: BioMed Central Country of Publication: England NLM ID: 101190741 Publication Model: Electronic Cited Medium: Internet ISSN: 1479-5876 (Electronic) Linking ISSN: 14795876 NLM ISO Abbreviation: J Transl Med Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: Development of a 3-day manufacturing method to generate CD19-CD20-CD22 trispecific CAR T-cells from whole blood.
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  Data: <searchLink fieldCode="AU" term="%22Vignola+I%22">Vignola I</searchLink>; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA.<br /><searchLink fieldCode="AU" term="%22Prochazkova+M%22">Prochazkova M</searchLink>; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA.<br /><searchLink fieldCode="AU" term="%22Shao+L%22">Shao L</searchLink>; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA.<br /><searchLink fieldCode="AU" term="%22Fuksenko+T%22">Fuksenko T</searchLink>; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA.<br /><searchLink fieldCode="AU" term="%22Sarkar+S%22">Sarkar S</searchLink>; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA.<br /><searchLink fieldCode="AU" term="%22Lei+H%22">Lei H</searchLink>; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA.<br /><searchLink fieldCode="AU" term="%22Jin+J%22">Jin J</searchLink>; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA.<br /><searchLink fieldCode="AU" term="%22Xiong+Y%22">Xiong Y</searchLink>; Caring Cross, Gaithersburg, MD, USA.<br /><searchLink fieldCode="AU" term="%22Xie+Y%22">Xie Y</searchLink>; Caring Cross, Gaithersburg, MD, USA.<br /><searchLink fieldCode="AU" term="%22Oparaocha+I%22">Oparaocha I</searchLink>; Caring Cross, Gaithersburg, MD, USA.<br /><searchLink fieldCode="AU" term="%22Slessareva+O%22">Slessareva O</searchLink>; Caring Cross, Gaithersburg, MD, USA.<br /><searchLink fieldCode="AU" term="%22Forrest+M%22">Forrest M</searchLink>; Caring Cross, Gaithersburg, MD, USA.<br /><searchLink fieldCode="AU" term="%22Orentas+R%22">Orentas R</searchLink>; Caring Cross, Gaithersburg, MD, USA.; Department Biochemistry and Molecular Biology, The Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.<br /><searchLink fieldCode="AU" term="%22Zhu+Z%22">Zhu Z</searchLink>; Caring Cross, Gaithersburg, MD, USA.<br /><searchLink fieldCode="AU" term="%22Dropulic+B%22">Dropulic B</searchLink>; Caring Cross, Gaithersburg, MD, USA.<br /><searchLink fieldCode="AU" term="%22Jin+P%22">Jin P</searchLink>; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA.<br /><searchLink fieldCode="AU" term="%22Somerville+RP%22">Somerville RP</searchLink>; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA.<br /><searchLink fieldCode="AU" term="%22Stroncek+DF%22">Stroncek DF</searchLink>; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA.<br /><searchLink fieldCode="AU" term="%22Song+HW%22">Song HW</searchLink>; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA.<br /><searchLink fieldCode="AU" term="%22Highfill+SL%22">Highfill SL</searchLink>; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA. Steven.Highfill@NIH.Gov.
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  Data: <searchLink fieldCode="JN" term="%22101190741%22">Journal of translational medicine</searchLink> [J Transl Med] 2026 Jul 15; Vol. 24 (1). <i>Date of Electronic Publication: </i>2026 Jul 15.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22BioMed+Central%22">BioMed Central </searchLink><i>Country of Publication: </i>England <i>NLM ID: </i>101190741 <i>Publication Model: </i>Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1479-5876 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2214795876%22">14795876 </searchLink><i>NLM ISO Abbreviation: </i>J Transl Med <i>Subsets: </i>MEDLINE
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        Value: 10.1186/s12967-026-08306-8
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        Text: English
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      – TitleFull: Development of a 3-day manufacturing method to generate CD19-CD20-CD22 trispecific CAR T-cells from whole blood.
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              Text: 2026 Jul 15
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              Y: 2026
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