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Cell types in the mouse cortex and hippocampus revealed by single-cell RNA-seq.

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Title: Cell types in the mouse cortex and hippocampus revealed by single-cell RNA-seq.
Authors: Zeisel, Amit, Muñoz-Manchado, Ana B., Codeluppi, Simone, Lönnerberg, Peter, La Manno, Gioele, Juréus, Anna, Marques, Sueli, Munguba, Hermany, Liqun He, Betsholtz, Christer, Rolny, Charlotte, Castelo-Branco, Gonçalo, Hjerling-Leffler, Jens, Linnarsson, Sten
Source: Science (pre-March 2025). 3/6/2015, Vol. 347 Issue 6226, p1138-1142. 5p.
Subjects: Cerebral cortex, Hippocampus (Brain), Laboratory mice, RNA sequencing, Cognitive ability, Neurons, Neuroglia, Somatosensory cortex
Abstract: The mammalian cerebral cortex supports cognitive functions such as sensorimotor integration, memory, and social behaviors. Normal brain function relies on a diverse set of differentiated cell types, including neurons, glia, and vasculature. Here, we have used large-scale single-cell RNA sequencing (RNA-seq) to classify cells in the mouse somatosensory cortex and hippocampal CA1 region. We found 47 molecularly distinct subclasses, comprising all known major cell types in the cortex. We identified numerous marker genes, which allowed alignment with known cell types, morphology, and location. We found a layer I interneuron expressing Pax6 and a distinct postmitotic oligodendrocyte subclass marked by Itpr2. Across the diversity of cortical cell types, transcription factors formed a complex, layered regulatory code, suggesting amechanismfor the maintenance of adult cell type identity. [ABSTRACT FROM AUTHOR]
Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Data: Cell types in the mouse cortex and hippocampus revealed by single-cell RNA-seq.
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  Data: <searchLink fieldCode="AR" term="%22Zeisel%2C+Amit%22">Zeisel, Amit</searchLink><br /><searchLink fieldCode="AR" term="%22Muñoz-Manchado%2C+Ana+B%2E%22">Muñoz-Manchado, Ana B.</searchLink><br /><searchLink fieldCode="AR" term="%22Codeluppi%2C+Simone%22">Codeluppi, Simone</searchLink><br /><searchLink fieldCode="AR" term="%22Lönnerberg%2C+Peter%22">Lönnerberg, Peter</searchLink><br /><searchLink fieldCode="AR" term="%22La+Manno%2C+Gioele%22">La Manno, Gioele</searchLink><br /><searchLink fieldCode="AR" term="%22Juréus%2C+Anna%22">Juréus, Anna</searchLink><br /><searchLink fieldCode="AR" term="%22Marques%2C+Sueli%22">Marques, Sueli</searchLink><br /><searchLink fieldCode="AR" term="%22Munguba%2C+Hermany%22">Munguba, Hermany</searchLink><br /><searchLink fieldCode="AR" term="%22Liqun+He%22">Liqun He</searchLink><br /><searchLink fieldCode="AR" term="%22Betsholtz%2C+Christer%22">Betsholtz, Christer</searchLink><br /><searchLink fieldCode="AR" term="%22Rolny%2C+Charlotte%22">Rolny, Charlotte</searchLink><br /><searchLink fieldCode="AR" term="%22Castelo-Branco%2C+Gonçalo%22">Castelo-Branco, Gonçalo</searchLink><br /><searchLink fieldCode="AR" term="%22Hjerling-Leffler%2C+Jens%22">Hjerling-Leffler, Jens</searchLink><br /><searchLink fieldCode="AR" term="%22Linnarsson%2C+Sten%22">Linnarsson, Sten</searchLink>
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  Data: <searchLink fieldCode="JN" term="%22Science+%28pre-March+2025%29%22">Science (pre-March 2025)</searchLink>. 3/6/2015, Vol. 347 Issue 6226, p1138-1142. 5p.
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  Data: <searchLink fieldCode="DE" term="%22Cerebral+cortex%22">Cerebral cortex</searchLink><br /><searchLink fieldCode="DE" term="%22Hippocampus+%28Brain%29%22">Hippocampus (Brain)</searchLink><br /><searchLink fieldCode="DE" term="%22Laboratory+mice%22">Laboratory mice</searchLink><br /><searchLink fieldCode="DE" term="%22RNA+sequencing%22">RNA sequencing</searchLink><br /><searchLink fieldCode="DE" term="%22Cognitive+ability%22">Cognitive ability</searchLink><br /><searchLink fieldCode="DE" term="%22Neurons%22">Neurons</searchLink><br /><searchLink fieldCode="DE" term="%22Neuroglia%22">Neuroglia</searchLink><br /><searchLink fieldCode="DE" term="%22Somatosensory+cortex%22">Somatosensory cortex</searchLink>
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  Data: The mammalian cerebral cortex supports cognitive functions such as sensorimotor integration, memory, and social behaviors. Normal brain function relies on a diverse set of differentiated cell types, including neurons, glia, and vasculature. Here, we have used large-scale single-cell RNA sequencing (RNA-seq) to classify cells in the mouse somatosensory cortex and hippocampal CA1 region. We found 47 molecularly distinct subclasses, comprising all known major cell types in the cortex. We identified numerous marker genes, which allowed alignment with known cell types, morphology, and location. We found a layer I interneuron expressing Pax6 and a distinct postmitotic oligodendrocyte subclass marked by Itpr2. Across the diversity of cortical cell types, transcription factors formed a complex, layered regulatory code, suggesting amechanismfor the maintenance of adult cell type identity. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=101388957
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        Text: English
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        PageCount: 5
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        Type: general
      – SubjectFull: Hippocampus (Brain)
        Type: general
      – SubjectFull: Laboratory mice
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      – SubjectFull: RNA sequencing
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      – SubjectFull: Cognitive ability
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      – SubjectFull: Neurons
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              Text: 3/6/2015
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