CRISPR adaptation biases explain preference for acquisition of foreign DNA.
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| Title: | CRISPR adaptation biases explain preference for acquisition of foreign DNA. |
|---|---|
| Authors: | Levy, Asaf, Goren, Moran G., Yosef, Ido, Auster, Oren, Manor, Miriam, Amitai, Gil, Edgar, Rotem, Qimron, Udi, Sorek, Rotem |
| Source: | Nature. 4/23/2015, Vol. 520 Issue 7548, p505-510. 6p. 1 Black and White Photograph, 4 Diagrams, 3 Charts, 6 Graphs. |
| Subjects: | Bacterial physiology, Immunity, DNA, Bacteriophages, Plasmids, DNA replication, Escherichia coli, Bacterial genetics |
| Abstract: | CRISPR-Cas (clustered, regularly interspaced short palindromic repeats coupled with CRISPR-associated proteins) is a bacterial immunity system that protects against invading phages or plasmids. In the process of CRISPR adaptation, short pieces of DNA ('spacers') are acquired from foreign elements and integrated into the CRISPR array. So far, it has remained a mystery how spacers are preferentially acquired from the foreign DNA while the self chromosome is avoided. Here we show that spacer acquisition is replication-dependent, and that DNA breaks formed at stalled replication forks promote spacer acquisition. Chromosomal hotspots of spacer acquisition were confined by Chi sites, which are sequence octamers highly enriched on the bacterial chromosome, suggesting that these sites limit spacer acquisition from self DNA. We further show that the avoidance of self is mediated by the RecBCD double-stranded DNA break repair complex. Our results suggest that, in Escherichia coli, acquisition of new spacers largely depends on RecBCD-mediated processing of double-stranded DNA breaks occurring primarily at replication forks, and that the preference for foreign DNA is achieved through the higher density of Chi sites on the self chromosome, in combination with the higher number of forks on the foreign DNA. This model explains the strong preference to acquire spacers both from high copy plasmids and from phages. [ABSTRACT FROM AUTHOR] |
| Copyright of Nature is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 102342271 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: CRISPR adaptation biases explain preference for acquisition of foreign DNA. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Levy%2C+Asaf%22">Levy, Asaf</searchLink><br /><searchLink fieldCode="AR" term="%22Goren%2C+Moran+G%2E%22">Goren, Moran G.</searchLink><br /><searchLink fieldCode="AR" term="%22Yosef%2C+Ido%22">Yosef, Ido</searchLink><br /><searchLink fieldCode="AR" term="%22Auster%2C+Oren%22">Auster, Oren</searchLink><br /><searchLink fieldCode="AR" term="%22Manor%2C+Miriam%22">Manor, Miriam</searchLink><br /><searchLink fieldCode="AR" term="%22Amitai%2C+Gil%22">Amitai, Gil</searchLink><br /><searchLink fieldCode="AR" term="%22Edgar%2C+Rotem%22">Edgar, Rotem</searchLink><br /><searchLink fieldCode="AR" term="%22Qimron%2C+Udi%22">Qimron, Udi</searchLink><br /><searchLink fieldCode="AR" term="%22Sorek%2C+Rotem%22">Sorek, Rotem</searchLink> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Nature%22">Nature</searchLink>. 4/23/2015, Vol. 520 Issue 7548, p505-510. 6p. 1 Black and White Photograph, 4 Diagrams, 3 Charts, 6 Graphs. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Bacterial+physiology%22">Bacterial physiology</searchLink><br /><searchLink fieldCode="DE" term="%22Immunity%22">Immunity</searchLink><br /><searchLink fieldCode="DE" term="%22DNA%22">DNA</searchLink><br /><searchLink fieldCode="DE" term="%22Bacteriophages%22">Bacteriophages</searchLink><br /><searchLink fieldCode="DE" term="%22Plasmids%22">Plasmids</searchLink><br /><searchLink fieldCode="DE" term="%22DNA+replication%22">DNA replication</searchLink><br /><searchLink fieldCode="DE" term="%22Escherichia+coli%22">Escherichia coli</searchLink><br /><searchLink fieldCode="DE" term="%22Bacterial+genetics%22">Bacterial genetics</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: CRISPR-Cas (clustered, regularly interspaced short palindromic repeats coupled with CRISPR-associated proteins) is a bacterial immunity system that protects against invading phages or plasmids. In the process of CRISPR adaptation, short pieces of DNA ('spacers') are acquired from foreign elements and integrated into the CRISPR array. So far, it has remained a mystery how spacers are preferentially acquired from the foreign DNA while the self chromosome is avoided. Here we show that spacer acquisition is replication-dependent, and that DNA breaks formed at stalled replication forks promote spacer acquisition. Chromosomal hotspots of spacer acquisition were confined by Chi sites, which are sequence octamers highly enriched on the bacterial chromosome, suggesting that these sites limit spacer acquisition from self DNA. We further show that the avoidance of self is mediated by the RecBCD double-stranded DNA break repair complex. Our results suggest that, in Escherichia coli, acquisition of new spacers largely depends on RecBCD-mediated processing of double-stranded DNA breaks occurring primarily at replication forks, and that the preference for foreign DNA is achieved through the higher density of Chi sites on the self chromosome, in combination with the higher number of forks on the foreign DNA. This model explains the strong preference to acquire spacers both from high copy plasmids and from phages. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Nature is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1038/nature14302 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 6 StartPage: 505 Subjects: – SubjectFull: Bacterial physiology Type: general – SubjectFull: Immunity Type: general – SubjectFull: DNA Type: general – SubjectFull: Bacteriophages Type: general – SubjectFull: Plasmids Type: general – SubjectFull: DNA replication Type: general – SubjectFull: Escherichia coli Type: general – SubjectFull: Bacterial genetics Type: general Titles: – TitleFull: CRISPR adaptation biases explain preference for acquisition of foreign DNA. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Levy, Asaf – PersonEntity: Name: NameFull: Goren, Moran G. – PersonEntity: Name: NameFull: Yosef, Ido – PersonEntity: Name: NameFull: Auster, Oren – PersonEntity: Name: NameFull: Manor, Miriam – PersonEntity: Name: NameFull: Amitai, Gil – PersonEntity: Name: NameFull: Edgar, Rotem – PersonEntity: Name: NameFull: Qimron, Udi – PersonEntity: Name: NameFull: Sorek, Rotem IsPartOfRelationships: – BibEntity: Dates: – D: 23 M: 04 Text: 4/23/2015 Type: published Y: 2015 Identifiers: – Type: issn-print Value: 00280836 Numbering: – Type: volume Value: 520 – Type: issue Value: 7548 Titles: – TitleFull: Nature Type: main |
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