Rs37972 and rs37973 single-nucleotide polymorphisms in the glucocorticoid-inducible 1 gene are not associated with asthma risk in a Saudi Arabian population.
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| Title: | Rs37972 and rs37973 single-nucleotide polymorphisms in the glucocorticoid-inducible 1 gene are not associated with asthma risk in a Saudi Arabian population. |
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| Authors: | Al-Muhsen, Saleh (AUTHOR), Vazquez-Tello, Alejandro (AUTHOR), Jamhawi, Amer (AUTHOR), Al-Dosari, Mohamed S. (AUTHOR), Mahboub, Bassam (AUTHOR), Iqbal, Nizam (AUTHOR), Temsah, Mohamed Hani (AUTHOR), Al-Eyadhy, Ayman (AUTHOR), Alharbi, Nasser (AUTHOR), Halwani, Rabih (AUTHOR) |
| Source: | Journal of Asthma. Mar2015, Vol. 52 Issue 2, p115-122. 8p. 4 Charts, 1 Graph. |
| Subjects: | Single nucleotide polymorphisms, Asthma risk factors, Glucocorticoids, Public health, Genotypes |
| Geographic Terms: | Saudi Arabia |
| Abstract: | Objective: Rs37972 and rs37973 variants in the glucocorticoid-induced transcript 1 gene have been associated with inhaled glucocorticosteroid responsiveness in asthmatics; however, some discrepancies have been also reported. This study aims to determine whether rs37972 and rs37973 SNPs are associated with asthma risk in Saudi Arabian asthmatics. Methods: Two-hundred seventy-one diagnosed asthmatics (3-65 years old) and 387 healthy control subjects of equivalent age were recruited. DNA from peripheral blood was purified, and genotyping of rs37972 and rs37973 SNPs was performed by PCR amplification of segments of interest, followed by Sanger sequencing. Results: The global frequencies of the minor (risk) alleles were 28% ('T' allele, rs37972) and 30% ('G' allele, rs37973). Yates-corrected Chi-square ( χ2) tests revealed significant differences between asthmatic and healthy groups, in allele frequencies for rs37973 SNP only ( χ2 = 3.98, Yates' p value = 0.046). Regarding genotype frequencies, a significant difference between asthmatic and healthy groups was observed for variant rs37972 only ( χ2 = 8.19, Yates' p value = 0.016). To determine a possible association of the minor 'T' and 'G' alleles with asthma, both the recessive and dominant genetic models were tested. For rs37973, none of the genotypes were significantly associated with asthma. Concerning rs37972, the dominant model (C/T + T/T versus C/C) indicated a significant 'protective' association with asthma, in which C/T + T/T individuals had lower odds of being asthmatics than C/C individuals (OR = 0.67; 95% CI = 0.48-0.94; p = 0.019*). Conclusions: The minor alleles 'T' and 'G' of rs37972 and rs37973 SNPs, respectively, were not significantly associated with increased asthma risk in asthma patients from Saudi Arabia. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Asthma is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
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| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 103223393 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Rs37972 and rs37973 single-nucleotide polymorphisms in the glucocorticoid-inducible 1 gene are not associated with asthma risk in a Saudi Arabian population. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Al-Muhsen%2C+Saleh%22">Al-Muhsen, Saleh</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Vazquez-Tello%2C+Alejandro%22">Vazquez-Tello, Alejandro</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Jamhawi%2C+Amer%22">Jamhawi, Amer</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Al-Dosari%2C+Mohamed+S%2E%22">Al-Dosari, Mohamed S.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mahboub%2C+Bassam%22">Mahboub, Bassam</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Iqbal%2C+Nizam%22">Iqbal, Nizam</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Temsah%2C+Mohamed+Hani%22">Temsah, Mohamed Hani</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Al-Eyadhy%2C+Ayman%22">Al-Eyadhy, Ayman</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Alharbi%2C+Nasser%22">Alharbi, Nasser</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Halwani%2C+Rabih%22">Halwani, Rabih</searchLink> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Asthma%22">Journal of Asthma</searchLink>. Mar2015, Vol. 52 Issue 2, p115-122. 8p. 4 Charts, 1 Graph. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Single+nucleotide+polymorphisms%22">Single nucleotide polymorphisms</searchLink><br /><searchLink fieldCode="DE" term="%22Asthma+risk+factors%22">Asthma risk factors</searchLink><br /><searchLink fieldCode="DE" term="%22Glucocorticoids%22">Glucocorticoids</searchLink><br /><searchLink fieldCode="DE" term="%22Public+health%22">Public health</searchLink><br /><searchLink fieldCode="DE" term="%22Genotypes%22">Genotypes</searchLink> – Name: SubjectGeographic Label: Geographic Terms Group: Su Data: <searchLink fieldCode="DE" term="%22Saudi+Arabia%22">Saudi Arabia</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Objective: Rs37972 and rs37973 variants in the glucocorticoid-induced transcript 1 gene have been associated with inhaled glucocorticosteroid responsiveness in asthmatics; however, some discrepancies have been also reported. This study aims to determine whether rs37972 and rs37973 SNPs are associated with asthma risk in Saudi Arabian asthmatics. Methods: Two-hundred seventy-one diagnosed asthmatics (3-65 years old) and 387 healthy control subjects of equivalent age were recruited. DNA from peripheral blood was purified, and genotyping of rs37972 and rs37973 SNPs was performed by PCR amplification of segments of interest, followed by Sanger sequencing. Results: The global frequencies of the minor (risk) alleles were 28% ('T' allele, rs37972) and 30% ('G' allele, rs37973). Yates-corrected Chi-square ( χ2) tests revealed significant differences between asthmatic and healthy groups, in allele frequencies for rs37973 SNP only ( χ2 = 3.98, Yates' p value = 0.046). Regarding genotype frequencies, a significant difference between asthmatic and healthy groups was observed for variant rs37972 only ( χ2 = 8.19, Yates' p value = 0.016). To determine a possible association of the minor 'T' and 'G' alleles with asthma, both the recessive and dominant genetic models were tested. For rs37973, none of the genotypes were significantly associated with asthma. Concerning rs37972, the dominant model (C/T + T/T versus C/C) indicated a significant 'protective' association with asthma, in which C/T + T/T individuals had lower odds of being asthmatics than C/C individuals (OR = 0.67; 95% CI = 0.48-0.94; p = 0.019*). Conclusions: The minor alleles 'T' and 'G' of rs37972 and rs37973 SNPs, respectively, were not significantly associated with increased asthma risk in asthma patients from Saudi Arabia. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Asthma is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=103223393 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3109/02770903.2014.955189 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 8 StartPage: 115 Subjects: – SubjectFull: Single nucleotide polymorphisms Type: general – SubjectFull: Asthma risk factors Type: general – SubjectFull: Glucocorticoids Type: general – SubjectFull: Public health Type: general – SubjectFull: Genotypes Type: general – SubjectFull: Saudi Arabia Type: general Titles: – TitleFull: Rs37972 and rs37973 single-nucleotide polymorphisms in the glucocorticoid-inducible 1 gene are not associated with asthma risk in a Saudi Arabian population. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Al-Muhsen, Saleh – PersonEntity: Name: NameFull: Vazquez-Tello, Alejandro – PersonEntity: Name: NameFull: Jamhawi, Amer – PersonEntity: Name: NameFull: Al-Dosari, Mohamed S. – PersonEntity: Name: NameFull: Mahboub, Bassam – PersonEntity: Name: NameFull: Iqbal, Nizam – PersonEntity: Name: NameFull: Temsah, Mohamed Hani – PersonEntity: Name: NameFull: Al-Eyadhy, Ayman – PersonEntity: Name: NameFull: Alharbi, Nasser – PersonEntity: Name: NameFull: Halwani, Rabih IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: Mar2015 Type: published Y: 2015 Identifiers: – Type: issn-print Value: 02770903 Numbering: – Type: volume Value: 52 – Type: issue Value: 2 Titles: – TitleFull: Journal of Asthma Type: main |
| ResultId | 1 |
