Proapoptotic effects of NF-[kappa]B on cisplatin-induced cell death in auditory cell line.
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| Title: | Proapoptotic effects of NF-[kappa]B on cisplatin-induced cell death in auditory cell line. |
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| Authors: | Chung W (AUTHOR), Boo SH (AUTHOR), Chung MK (AUTHOR), Lee H (AUTHOR), Cho Y (AUTHOR), Hong SH (AUTHOR) |
| Source: | Acta Oto-Laryngologica. Oct2008, Vol. 128 Issue 10, p1063-1070. 8p. |
| Abstract: | CONCLUSIONS: The findings suggest that nuclear factor (NF)-kappaB is activated by cisplatin and plays a proapoptotic role during cell death in the auditory cell line HEI-OC1. OBJECTIVES: Cisplatin is a very effective antineoplastic drug but in high doses it shows ototoxicity by inducing apoptosis of hair cells in the cochlea. NF-kappaB is a transcription factor regulating apoptosis in many organs and tissues. This study examined the role of NF-kappaB in the apoptotic pathway induced by cisplatin in the auditory cell line, HEI-OC1. MATERIALS AND METHODS: Apoptotic cell death was identified by measuring caspase 3 activity and immunostaining with anti-caspase 3 antibody after cisplatin treatment (50 microM) for 24 h. To investigate the role of NF-kappaB in apoptotic cell death, HEI-OC1 cells treated with 50 microM of cisplatin were immunostained with anti-NF-kappaB (p65) antibody. Two different NF-kappaB inhibitors, Bay 11-7085 and SN-50, were co-incubated with cisplatin (50 microM) for 24 h and caspase 3 activity was assayed. RESULTS: Immunostaining with anti-caspase 3 antibody and caspase 3 assay showed that cisplatin induced apoptosis in HEI-OC1 cells. After cisplatin treatment, NF-kappaB (p65) was activated to translocate from the cytoplasm into the nucleus. Co-treatment with NF-kappaB inhibitors reduced the cisplatin-induced apoptosis of HEI-OC1 cells. [ABSTRACT FROM AUTHOR] |
| Copyright of Acta Oto-Laryngologica is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 105554296 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Proapoptotic effects of NF-[kappa]B on cisplatin-induced cell death in auditory cell line. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Chung+W%22">Chung W</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Boo+SH%22">Boo SH</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chung+MK%22">Chung MK</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lee+H%22">Lee H</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Cho+Y%22">Cho Y</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hong+SH%22">Hong SH</searchLink> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Acta+Oto-Laryngologica%22">Acta Oto-Laryngologica</searchLink>. Oct2008, Vol. 128 Issue 10, p1063-1070. 8p. – Name: Abstract Label: Abstract Group: Ab Data: CONCLUSIONS: The findings suggest that nuclear factor (NF)-kappaB is activated by cisplatin and plays a proapoptotic role during cell death in the auditory cell line HEI-OC1. OBJECTIVES: Cisplatin is a very effective antineoplastic drug but in high doses it shows ototoxicity by inducing apoptosis of hair cells in the cochlea. NF-kappaB is a transcription factor regulating apoptosis in many organs and tissues. This study examined the role of NF-kappaB in the apoptotic pathway induced by cisplatin in the auditory cell line, HEI-OC1. MATERIALS AND METHODS: Apoptotic cell death was identified by measuring caspase 3 activity and immunostaining with anti-caspase 3 antibody after cisplatin treatment (50 microM) for 24 h. To investigate the role of NF-kappaB in apoptotic cell death, HEI-OC1 cells treated with 50 microM of cisplatin were immunostained with anti-NF-kappaB (p65) antibody. Two different NF-kappaB inhibitors, Bay 11-7085 and SN-50, were co-incubated with cisplatin (50 microM) for 24 h and caspase 3 activity was assayed. RESULTS: Immunostaining with anti-caspase 3 antibody and caspase 3 assay showed that cisplatin induced apoptosis in HEI-OC1 cells. After cisplatin treatment, NF-kappaB (p65) was activated to translocate from the cytoplasm into the nucleus. Co-treatment with NF-kappaB inhibitors reduced the cisplatin-induced apoptosis of HEI-OC1 cells. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Acta Oto-Laryngologica is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1080/00016480701881811 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 8 StartPage: 1063 Titles: – TitleFull: Proapoptotic effects of NF-[kappa]B on cisplatin-induced cell death in auditory cell line. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Chung W – PersonEntity: Name: NameFull: Boo SH – PersonEntity: Name: NameFull: Chung MK – PersonEntity: Name: NameFull: Lee H – PersonEntity: Name: NameFull: Cho Y – PersonEntity: Name: NameFull: Hong SH IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 10 Text: Oct2008 Type: published Y: 2008 Identifiers: – Type: issn-print Value: 00016489 Numbering: – Type: volume Value: 128 – Type: issue Value: 10 Titles: – TitleFull: Acta Oto-Laryngologica Type: main |
| ResultId | 1 |
