Differential gene expression profiles in salicylate ototoxicity of the mouse.
Saved in:
| Title: | Differential gene expression profiles in salicylate ototoxicity of the mouse. |
|---|---|
| Authors: | Im GJ (AUTHOR), Jung HH (AUTHOR), Chae SW (AUTHOR), Cho WS (AUTHOR), Kim SJ (AUTHOR) |
| Source: | Acta Oto-Laryngologica. May2007, Vol. 127 Issue 5, p459-469. 11p. |
| Abstract: | Conclusion. This study demonstrated differential gene expression profiles in salicylate ototoxicity with oligonucleotide microarray. This study may also provide basic information on candidate genes associated with hearing loss and/or tinnitus or recovery after salicylate-induced cochlear dysfunction. Objectives: Salicylate ototoxicity is accompanied by temporary hearing loss and tinnitus. The purpose of the present study was to evaluate the gene expression profiles in the mouse cochlea with salicylate ototoxicity using DNA microarray. Materials and methods: The subject mice were injected intraperitoneally with 400 mg/kg of sodium salicylate; an approximate 30 dB threshold shift that was observed by auditory brainstem response was achieved 3 h after an injection of sodium salicylate and the hearing threshold returned to within normal range at 3 days. Differential gene expression profiles at 3 h after salicylate injection in comparison to the normal cochlea were analyzed with DNA microarray technology. Results: No ultrastructural changes in the mice cochlea were observed by TEM at 3 h after salicylate injection. Microarray revealed that 87 genes were up-regulated twofold or more in the mouse cochlea with salicylate ototoxicity in comparison to the normal cochlea. Among these genes, increased expression levels of 30 functional genes were confirmed by semi-quantitative RT-PCR. [ABSTRACT FROM AUTHOR] |
| Copyright of Acta Oto-Laryngologica is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
|---|---|
| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 106106348 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Differential gene expression profiles in salicylate ototoxicity of the mouse. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Im+GJ%22">Im GJ</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Jung+HH%22">Jung HH</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chae+SW%22">Chae SW</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Cho+WS%22">Cho WS</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kim+SJ%22">Kim SJ</searchLink> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Acta+Oto-Laryngologica%22">Acta Oto-Laryngologica</searchLink>. May2007, Vol. 127 Issue 5, p459-469. 11p. – Name: Abstract Label: Abstract Group: Ab Data: Conclusion. This study demonstrated differential gene expression profiles in salicylate ototoxicity with oligonucleotide microarray. This study may also provide basic information on candidate genes associated with hearing loss and/or tinnitus or recovery after salicylate-induced cochlear dysfunction. Objectives: Salicylate ototoxicity is accompanied by temporary hearing loss and tinnitus. The purpose of the present study was to evaluate the gene expression profiles in the mouse cochlea with salicylate ototoxicity using DNA microarray. Materials and methods: The subject mice were injected intraperitoneally with 400 mg/kg of sodium salicylate; an approximate 30 dB threshold shift that was observed by auditory brainstem response was achieved 3 h after an injection of sodium salicylate and the hearing threshold returned to within normal range at 3 days. Differential gene expression profiles at 3 h after salicylate injection in comparison to the normal cochlea were analyzed with DNA microarray technology. Results: No ultrastructural changes in the mice cochlea were observed by TEM at 3 h after salicylate injection. Microarray revealed that 87 genes were up-regulated twofold or more in the mouse cochlea with salicylate ototoxicity in comparison to the normal cochlea. Among these genes, increased expression levels of 30 functional genes were confirmed by semi-quantitative RT-PCR. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Acta Oto-Laryngologica is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=106106348 |
| RecordInfo | BibRecord: BibEntity: Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 459 Titles: – TitleFull: Differential gene expression profiles in salicylate ototoxicity of the mouse. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Im GJ – PersonEntity: Name: NameFull: Jung HH – PersonEntity: Name: NameFull: Chae SW – PersonEntity: Name: NameFull: Cho WS – PersonEntity: Name: NameFull: Kim SJ IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 05 Text: May2007 Type: published Y: 2007 Identifiers: – Type: issn-print Value: 00016489 Numbering: – Type: volume Value: 127 – Type: issue Value: 5 Titles: – TitleFull: Acta Oto-Laryngologica Type: main |
| ResultId | 1 |
