IL-1beta promotes the ciliogenesis of human middle ear epithelial cells: possible linkage with the expression of mucin gene 8.
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| Title: | IL-1beta promotes the ciliogenesis of human middle ear epithelial cells: possible linkage with the expression of mucin gene 8. |
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| Authors: | Choi JY (AUTHOR), Kim J (AUTHOR), Kim C (AUTHOR), Ho JS (AUTHOR), Lee K (AUTHOR), Yoo J (AUTHOR), Ahn YE (AUTHOR), Yoon J (AUTHOR) |
| Source: | Acta Oto-Laryngologica. Mar2005, Vol. 125 Issue 3, p260-265. 6p. |
| Abstract: | CONCLUSIONS: These findings suggest that IL-1beta, increases ciliogenesis in NHMEE cells and that MUC8 protein may play a role in this process. OBJECTIVES: The morphology of the middle ear epithelium changes under various pathologic conditions. We compared the density of ciliated cells and the level of mucin gene 8 (MUC8) mRNA in the middle ear epithelial cells of mucoid otitis media (MOM) patients with those of normal controls. We also investigated the effect of IL-1beta on the ciliogenesis and expression of MUC8 mRNA in cultured normal human middle ear epithelial (NHMEE) cells. In addition, we localized the MUC8 protein in cultured NHMEE cells. MATERIAL AND METHODS: The surface morphology of NHMEE cells was examined using scanning electron microscopy. Reverse transcriptase polymerase chain reaction was performed to determine the level of MUC8 mRNA expression. After synthesis of MUC8 polyclonal antibody, we performed immunohistochemical staining. RESULTS: The density of ciliated cells was increased in the middle ear epithelium of both MOM patients and IL-1beta-treated cultures. Similarly, the expression of MUC8 was increased in both cases. MUC8 antibody was primarily stained on the ciliated cells of NHMEE cells. [ABSTRACT FROM AUTHOR] |
| Copyright of Acta Oto-Laryngologica is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 106313114 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: IL-1beta promotes the ciliogenesis of human middle ear epithelial cells: possible linkage with the expression of mucin gene 8. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Choi+JY%22">Choi JY</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kim+J%22">Kim J</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kim+C%22">Kim C</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ho+JS%22">Ho JS</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lee+K%22">Lee K</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yoo+J%22">Yoo J</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ahn+YE%22">Ahn YE</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yoon+J%22">Yoon J</searchLink> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Acta+Oto-Laryngologica%22">Acta Oto-Laryngologica</searchLink>. Mar2005, Vol. 125 Issue 3, p260-265. 6p. – Name: Abstract Label: Abstract Group: Ab Data: CONCLUSIONS: These findings suggest that IL-1beta, increases ciliogenesis in NHMEE cells and that MUC8 protein may play a role in this process. OBJECTIVES: The morphology of the middle ear epithelium changes under various pathologic conditions. We compared the density of ciliated cells and the level of mucin gene 8 (MUC8) mRNA in the middle ear epithelial cells of mucoid otitis media (MOM) patients with those of normal controls. We also investigated the effect of IL-1beta on the ciliogenesis and expression of MUC8 mRNA in cultured normal human middle ear epithelial (NHMEE) cells. In addition, we localized the MUC8 protein in cultured NHMEE cells. MATERIAL AND METHODS: The surface morphology of NHMEE cells was examined using scanning electron microscopy. Reverse transcriptase polymerase chain reaction was performed to determine the level of MUC8 mRNA expression. After synthesis of MUC8 polyclonal antibody, we performed immunohistochemical staining. RESULTS: The density of ciliated cells was increased in the middle ear epithelium of both MOM patients and IL-1beta-treated cultures. Similarly, the expression of MUC8 was increased in both cases. MUC8 antibody was primarily stained on the ciliated cells of NHMEE cells. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Acta Oto-Laryngologica is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=106313114 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1080/00016480410022985 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 6 StartPage: 260 Titles: – TitleFull: IL-1beta promotes the ciliogenesis of human middle ear epithelial cells: possible linkage with the expression of mucin gene 8. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Choi JY – PersonEntity: Name: NameFull: Kim J – PersonEntity: Name: NameFull: Kim C – PersonEntity: Name: NameFull: Ho JS – PersonEntity: Name: NameFull: Lee K – PersonEntity: Name: NameFull: Yoo J – PersonEntity: Name: NameFull: Ahn YE – PersonEntity: Name: NameFull: Yoon J IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: Mar2005 Type: published Y: 2005 Identifiers: – Type: issn-print Value: 00016489 Numbering: – Type: volume Value: 125 – Type: issue Value: 3 Titles: – TitleFull: Acta Oto-Laryngologica Type: main |
| ResultId | 1 |
