Inhibition of melanogenesis in murine B16/F10 melanoma cells by Ligusticum sinensis Oliv.
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| Title: | Inhibition of melanogenesis in murine B16/F10 melanoma cells by Ligusticum sinensis Oliv. |
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| Authors: | Wu C (AUTHOR), Pang JS (AUTHOR), Huang S (AUTHOR) |
| Source: | American Journal of Chinese Medicine. 2006, Vol. 34 Issue 3, p523-533. 11p. |
| Abstract: | Ligusticum sinensis Oliv. (LSO) is an herbal drug commonly used as a topical treatment of epidermal hyperdepigmentation in Chinese medicine. However, the mechanism underlying the depigmentation by LSO is still unclear. The purpose of this study was to investigate the effects of LSO on the process of melanogenesis and its possible underlying mechanism. Suppressed DOPA oxidase activity of mushroom tyrosinase was first noted when incubated with aqueous extracts of LSO, demonstrating the direct inhibitory effect of LSO on mushroom tyrosinase. Further experiments were carried out in murine B16/F10 melanoma cells and the effects of LSO extract on melanin formation, tyrosinase activity and tyrosinase gene expression were tested. Under conditions without affecting the viability of murine B16/F10 melanoma cells, LSO extract significantly reduced the cellular melanin content in a dose-dependent manner. The DOPA oxidase activity of tyrosinase in B16/F10 cells was dose-dependently inhibited by LSO treatment, possibly mediated by the suppressed tyrosinase mRNA expression in LSO-treated B16/F10 cells. In conclusion, the inhibitory effect of LSO on melanogenesis is likely associated with decreased DOPA oxidase activity of tyrosinase that is most likely the result of the down-regulation of tyrosinase mRNA expression. [ABSTRACT FROM AUTHOR] |
| Copyright of American Journal of Chinese Medicine is the property of World Scientific Publishing Company and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 106324888 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Inhibition of melanogenesis in murine B16/F10 melanoma cells by Ligusticum sinensis Oliv. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Wu+C%22">Wu C</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pang+JS%22">Pang JS</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Huang+S%22">Huang S</searchLink> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22American+Journal+of+Chinese+Medicine%22">American Journal of Chinese Medicine</searchLink>. 2006, Vol. 34 Issue 3, p523-533. 11p. – Name: Abstract Label: Abstract Group: Ab Data: Ligusticum sinensis Oliv. (LSO) is an herbal drug commonly used as a topical treatment of epidermal hyperdepigmentation in Chinese medicine. However, the mechanism underlying the depigmentation by LSO is still unclear. The purpose of this study was to investigate the effects of LSO on the process of melanogenesis and its possible underlying mechanism. Suppressed DOPA oxidase activity of mushroom tyrosinase was first noted when incubated with aqueous extracts of LSO, demonstrating the direct inhibitory effect of LSO on mushroom tyrosinase. Further experiments were carried out in murine B16/F10 melanoma cells and the effects of LSO extract on melanin formation, tyrosinase activity and tyrosinase gene expression were tested. Under conditions without affecting the viability of murine B16/F10 melanoma cells, LSO extract significantly reduced the cellular melanin content in a dose-dependent manner. The DOPA oxidase activity of tyrosinase in B16/F10 cells was dose-dependently inhibited by LSO treatment, possibly mediated by the suppressed tyrosinase mRNA expression in LSO-treated B16/F10 cells. In conclusion, the inhibitory effect of LSO on melanogenesis is likely associated with decreased DOPA oxidase activity of tyrosinase that is most likely the result of the down-regulation of tyrosinase mRNA expression. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of American Journal of Chinese Medicine is the property of World Scientific Publishing Company and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 523 Titles: – TitleFull: Inhibition of melanogenesis in murine B16/F10 melanoma cells by Ligusticum sinensis Oliv. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Wu C – PersonEntity: Name: NameFull: Pang JS – PersonEntity: Name: NameFull: Huang S IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 06 Text: 2006 Type: published Y: 2006 Identifiers: – Type: issn-print Value: 0192415X Numbering: – Type: volume Value: 34 – Type: issue Value: 3 Titles: – TitleFull: American Journal of Chinese Medicine Type: main |
| ResultId | 1 |
