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Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics.

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Title: Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics.
Authors: Dong Li, Lin Shao, Bi-Chang Chen, Xi Zhang, Mingshu Zhang, Moses, Brian, Milkie, Daniel E., Beach, Jordan R., Hammer III, John A., Pasham, Mithun, Kirchhausen, Tomas, Baird, Michelle A., Davidson, Michael W., Pingyong Xu, Betzig, Eric
Source: Science (pre-March 2025). 8/28/2015, Vol. 349 Issue 6251, p500-1-00-10. 10p.
Subjects: Fluorescence microscopy, Cytoskeleton, Endocytosis, Cell imaging, Golgi apparatus
Abstract: Super-resolution fluorescence microscopy is distinct among nanoscale imaging tools in its ability to image protein dynamics in living cells. Structured illumination microscopy (SIM) stands out in this regard because of its high speed and low illumination intensities, but typically offers only a twofold resolution gain. We extended the resolution of live-cell SIM through two approaches: ultrahigh numerical aperture SIM at 84-nanometer lateral resolution for more than 100 multicolor frames, and nonlinear SIM with patterned activation at 45- to 62-nanometer resolution for approximately 20 to 40 frames. We applied these approaches to image dynamics near the plasma membrane of spatially resolved assemblies of clathrin and caveolin, Rab5a in early endosomes, and a-actinin, often in relationship to cortical actin. In addition, we examined mitochondria, actin, and the Golgi apparatus dynamics in three dimensions. [ABSTRACT FROM AUTHOR]
Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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PubType: Academic Journal
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  Data: Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics.
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  Data: <searchLink fieldCode="AR" term="%22Dong+Li%22">Dong Li</searchLink><br /><searchLink fieldCode="AR" term="%22Lin+Shao%22">Lin Shao</searchLink><br /><searchLink fieldCode="AR" term="%22Bi-Chang+Chen%22">Bi-Chang Chen</searchLink><br /><searchLink fieldCode="AR" term="%22Xi+Zhang%22">Xi Zhang</searchLink><br /><searchLink fieldCode="AR" term="%22Mingshu+Zhang%22">Mingshu Zhang</searchLink><br /><searchLink fieldCode="AR" term="%22Moses%2C+Brian%22">Moses, Brian</searchLink><br /><searchLink fieldCode="AR" term="%22Milkie%2C+Daniel+E%2E%22">Milkie, Daniel E.</searchLink><br /><searchLink fieldCode="AR" term="%22Beach%2C+Jordan+R%2E%22">Beach, Jordan R.</searchLink><br /><searchLink fieldCode="AR" term="%22Hammer+III%2C+John+A%2E%22">Hammer III, John A.</searchLink><br /><searchLink fieldCode="AR" term="%22Pasham%2C+Mithun%22">Pasham, Mithun</searchLink><br /><searchLink fieldCode="AR" term="%22Kirchhausen%2C+Tomas%22">Kirchhausen, Tomas</searchLink><br /><searchLink fieldCode="AR" term="%22Baird%2C+Michelle+A%2E%22">Baird, Michelle A.</searchLink><br /><searchLink fieldCode="AR" term="%22Davidson%2C+Michael+W%2E%22">Davidson, Michael W.</searchLink><br /><searchLink fieldCode="AR" term="%22Pingyong+Xu%22">Pingyong Xu</searchLink><br /><searchLink fieldCode="AR" term="%22Betzig%2C+Eric%22">Betzig, Eric</searchLink>
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  Data: <searchLink fieldCode="JN" term="%22Science+%28pre-March+2025%29%22">Science (pre-March 2025)</searchLink>. 8/28/2015, Vol. 349 Issue 6251, p500-1-00-10. 10p.
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  Data: <searchLink fieldCode="DE" term="%22Fluorescence+microscopy%22">Fluorescence microscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Cytoskeleton%22">Cytoskeleton</searchLink><br /><searchLink fieldCode="DE" term="%22Endocytosis%22">Endocytosis</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+imaging%22">Cell imaging</searchLink><br /><searchLink fieldCode="DE" term="%22Golgi+apparatus%22">Golgi apparatus</searchLink>
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  Data: Super-resolution fluorescence microscopy is distinct among nanoscale imaging tools in its ability to image protein dynamics in living cells. Structured illumination microscopy (SIM) stands out in this regard because of its high speed and low illumination intensities, but typically offers only a twofold resolution gain. We extended the resolution of live-cell SIM through two approaches: ultrahigh numerical aperture SIM at 84-nanometer lateral resolution for more than 100 multicolor frames, and nonlinear SIM with patterned activation at 45- to 62-nanometer resolution for approximately 20 to 40 frames. We applied these approaches to image dynamics near the plasma membrane of spatially resolved assemblies of clathrin and caveolin, Rab5a in early endosomes, and a-actinin, often in relationship to cortical actin. In addition, we examined mitochondria, actin, and the Golgi apparatus dynamics in three dimensions. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=109156888
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        Value: 10.1126/science.aab3500
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        Text: English
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      – SubjectFull: Fluorescence microscopy
        Type: general
      – SubjectFull: Cytoskeleton
        Type: general
      – SubjectFull: Endocytosis
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      – SubjectFull: Cell imaging
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      – SubjectFull: Golgi apparatus
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              Text: 8/28/2015
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