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Gene essentiality and synthetic lethality in haploid human cells.

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Title: Gene essentiality and synthetic lethality in haploid human cells.
Authors: Blomen, Vincent A., Májek, Peter, Jae, Lucas T., Bigenzahn, Johannes W., Nieuwenhuis, Joppe, Staring, Jacqueline, Sacco, Roberto, van Diemen, Ferdy R., Olk, Nadine, Stukalov, Alexey, Marceau, Caleb, Janssen, Hans, Carette, Jan E., Bennett, Keiryn L., Colinge, Jacques, Superti-Furga, Giulio, Brummelkamp, Thijn R.
Source: Science (pre-March 2025). 11/27/2015, Vol. 350 Issue 6264, p1092-1098. 5p.
Subjects: Genes, Haploidy, Cells, Genomics, Cytology, Biological crosstalk, Cellular signal transduction
Abstract: Although the genes essential for life have been identified in less complex model organisms, their elucidation in human cells has been hindered by technical barriers. We used extensive mutagenesis in haploid human cells to identify approximately 2000 genes required for optimal fitness under culture conditions. To study the principles of genetic interactions in human cells, we created a synthetic lethality network focused on the secretory pathway based exclusively on mutations. This revealed a genetic cross-talk governing Golgi homeostasis, an additional subunit of the human oligosaccharyltransferase complex, and a phosphatidylinositol 4-kinase b adaptor hijacked by viruses. The synthetic lethality map parallels observations made in yeast and projects a route forward to reveal genetic networks in diverse aspects of human cell biology. [ABSTRACT FROM AUTHOR]
Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Data: Gene essentiality and synthetic lethality in haploid human cells.
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  Data: <searchLink fieldCode="AR" term="%22Blomen%2C+Vincent+A%2E%22">Blomen, Vincent A.</searchLink><br /><searchLink fieldCode="AR" term="%22Májek%2C+Peter%22">Májek, Peter</searchLink><br /><searchLink fieldCode="AR" term="%22Jae%2C+Lucas+T%2E%22">Jae, Lucas T.</searchLink><br /><searchLink fieldCode="AR" term="%22Bigenzahn%2C+Johannes+W%2E%22">Bigenzahn, Johannes W.</searchLink><br /><searchLink fieldCode="AR" term="%22Nieuwenhuis%2C+Joppe%22">Nieuwenhuis, Joppe</searchLink><br /><searchLink fieldCode="AR" term="%22Staring%2C+Jacqueline%22">Staring, Jacqueline</searchLink><br /><searchLink fieldCode="AR" term="%22Sacco%2C+Roberto%22">Sacco, Roberto</searchLink><br /><searchLink fieldCode="AR" term="%22van+Diemen%2C+Ferdy+R%2E%22">van Diemen, Ferdy R.</searchLink><br /><searchLink fieldCode="AR" term="%22Olk%2C+Nadine%22">Olk, Nadine</searchLink><br /><searchLink fieldCode="AR" term="%22Stukalov%2C+Alexey%22">Stukalov, Alexey</searchLink><br /><searchLink fieldCode="AR" term="%22Marceau%2C+Caleb%22">Marceau, Caleb</searchLink><br /><searchLink fieldCode="AR" term="%22Janssen%2C+Hans%22">Janssen, Hans</searchLink><br /><searchLink fieldCode="AR" term="%22Carette%2C+Jan+E%2E%22">Carette, Jan E.</searchLink><br /><searchLink fieldCode="AR" term="%22Bennett%2C+Keiryn+L%2E%22">Bennett, Keiryn L.</searchLink><br /><searchLink fieldCode="AR" term="%22Colinge%2C+Jacques%22">Colinge, Jacques</searchLink><br /><searchLink fieldCode="AR" term="%22Superti-Furga%2C+Giulio%22">Superti-Furga, Giulio</searchLink><br /><searchLink fieldCode="AR" term="%22Brummelkamp%2C+Thijn+R%2E%22">Brummelkamp, Thijn R.</searchLink>
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  Data: <searchLink fieldCode="JN" term="%22Science+%28pre-March+2025%29%22">Science (pre-March 2025)</searchLink>. 11/27/2015, Vol. 350 Issue 6264, p1092-1098. 5p.
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  Data: <searchLink fieldCode="DE" term="%22Genes%22">Genes</searchLink><br /><searchLink fieldCode="DE" term="%22Haploidy%22">Haploidy</searchLink><br /><searchLink fieldCode="DE" term="%22Cells%22">Cells</searchLink><br /><searchLink fieldCode="DE" term="%22Genomics%22">Genomics</searchLink><br /><searchLink fieldCode="DE" term="%22Cytology%22">Cytology</searchLink><br /><searchLink fieldCode="DE" term="%22Biological+crosstalk%22">Biological crosstalk</searchLink><br /><searchLink fieldCode="DE" term="%22Cellular+signal+transduction%22">Cellular signal transduction</searchLink>
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  Data: Although the genes essential for life have been identified in less complex model organisms, their elucidation in human cells has been hindered by technical barriers. We used extensive mutagenesis in haploid human cells to identify approximately 2000 genes required for optimal fitness under culture conditions. To study the principles of genetic interactions in human cells, we created a synthetic lethality network focused on the secretory pathway based exclusively on mutations. This revealed a genetic cross-talk governing Golgi homeostasis, an additional subunit of the human oligosaccharyltransferase complex, and a phosphatidylinositol 4-kinase b adaptor hijacked by viruses. The synthetic lethality map parallels observations made in yeast and projects a route forward to reveal genetic networks in diverse aspects of human cell biology. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=111219466
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      – SubjectFull: Haploidy
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      – SubjectFull: Cells
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      – SubjectFull: Cellular signal transduction
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