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Effects and Mechanism of Combination of Rhein and Danshensu in the Treatment of Chronic Kidney Disease.

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Title: Effects and Mechanism of Combination of Rhein and Danshensu in the Treatment of Chronic Kidney Disease.
Authors: Guan, Yue, Wu, Xiao-Xiao, Duan, Jia-Lin, Yin, Ying, Guo, Chao, Wei, Guo, Wang, Yan-Hua, Zhu, Yan-Rong, Weng, Yan, Xi, Miao-Miao, Wen, Ai-Dong
Source: American Journal of Chinese Medicine. 2015, Vol. 43 Issue 7, p1381-1400. 20p. 8 Graphs.
Subjects: Treatment of chronic kidney failure, Analysis of variance, Animal experimentation, Cytokines, Diagnostic imaging, Immunohistochemistry, Interleukins, Medicinal plants, Botanic medicine, Chinese medicine, Pathology, Probability theory, Rats, Research funding, RNA, Ultrasonic imaging, Western immunoblotting, Treatment effectiveness, Data analysis software, Nephrectomy
Abstract: Traditional Chinese medicine (TCM) plays a systemic role in disease treatment, targeting multiple etiological factors simultaneously. Based on clinical experience, rhubarb and Salvia miltiorrhiza are commonly prescribed together for the treatment of chronic kidney disease (CKD) and have been proven to be very effective. However, the rationale of the combination remains unclear. The major active ingredients of these two herbs are rhein (RH) and danshensu (DSS), respectively. The aim of this paper is to investigate the renoprotective effects of RH and DSS in vitro and in vivo, and the underlying mechanism. A total of 5/6 nephrectomy rats and HK-2 cells were subjected to chronic renal injury. The combination of RH and DSS conferred a protective effect, as shown by a significant improvement in the renal function, blood supply, and fibrotic degree. Proinflammatory cytokines and adhesion molecules were suppressed by RH and DSS through NK-B signaling. The combination also inhibited apoptosis by up-regulating Bcl-2 and down-regulating Bax. Inhibiting the TGF-/Smad3 pathway was at least in part involved in the antifibrotic mechanism of the combination treatment of RH and DSS. This study demonstrates for the first time the renoprotective effect and the mechanism of RH and DSS combination on chronic renal injury. It could provide experimental evidence to support the rationality of the combinatorial use of TCM in clinical practices. [ABSTRACT FROM AUTHOR]
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Database: Psychology and Behavioral Sciences Collection
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Abstract:Traditional Chinese medicine (TCM) plays a systemic role in disease treatment, targeting multiple etiological factors simultaneously. Based on clinical experience, rhubarb and Salvia miltiorrhiza are commonly prescribed together for the treatment of chronic kidney disease (CKD) and have been proven to be very effective. However, the rationale of the combination remains unclear. The major active ingredients of these two herbs are rhein (RH) and danshensu (DSS), respectively. The aim of this paper is to investigate the renoprotective effects of RH and DSS in vitro and in vivo, and the underlying mechanism. A total of 5/6 nephrectomy rats and HK-2 cells were subjected to chronic renal injury. The combination of RH and DSS conferred a protective effect, as shown by a significant improvement in the renal function, blood supply, and fibrotic degree. Proinflammatory cytokines and adhesion molecules were suppressed by RH and DSS through NK-B signaling. The combination also inhibited apoptosis by up-regulating Bcl-2 and down-regulating Bax. Inhibiting the TGF-/Smad3 pathway was at least in part involved in the antifibrotic mechanism of the combination treatment of RH and DSS. This study demonstrates for the first time the renoprotective effect and the mechanism of RH and DSS combination on chronic renal injury. It could provide experimental evidence to support the rationality of the combinatorial use of TCM in clinical practices. [ABSTRACT FROM AUTHOR]
ISSN:0192415X
DOI:10.1142/S0192415X15500780