Ethanol Extract of Fructus ligustri lucidi Increased Circulating 1,25(OH)2D3 Levels, but Did Not Improve Calcium Balance in Mature Ovariectomized Rats.
Saved in:
| Title: | Ethanol Extract of Fructus ligustri lucidi Increased Circulating 1,25(OH) |
|---|---|
| Authors: | Dong, Xiao-Li, Cao, Si-Si, Zhou, Li-Ping, Denney, Liya, Wong, Man-Sau, Feng, Hao-Tian |
| Source: | American Journal of Chinese Medicine. 2016, Vol. 44 Issue 6, p1237-1253. 17p. 2 Charts, 5 Graphs. |
| Subjects: | Animal experimentation, Calcium, Diet, Enzyme-linked immunosorbent assay, Polymerase chain reaction, Rats, Research funding, Statistics, T-test (Statistics), Western immunoblotting, Plant extracts, Data analysis, Cholecalciferol, In vitro studies, One-way analysis of variance |
| Abstract: | Our previous studies found that different extracts or fractions of Fructus ligustri lucidi (FLL) played different roles in altering the regulation of bone and mineral metabolism in different animal models. The present study was designed to compare the actions of FLL ethanol (EE) and water extracts (WE) on bone and mineral metabolism in a 6-month-old mature ovariectomized (OVX) rat model. Our results showed that FLL extracts did not significantly improve systematic Ca balance in mature OVX rats. However, EE, but not WE treatment, significantly increased serum 1,25(OH)2D3 levels in mature OVX rats. An in vitro study using human proximal tubule (HKC-8) cells showed that EE, but not WE, significantly enhanced renal 25-dihydroxyvitamin D3-1-hydroxylase (1-OHase) mRNA expressions and simultaneously repressed renal 25-dihydroxyvitamin D3-24-hydroxylase (24-OHase) mRNA expressions. Further investigation indicated that EE could significantly induce the protein expression of 1-OHase, but did not alter 24-OHase expression in HKC-8 cells. Our results demonstrated that EE increased circulating 1,25(OH)2D3 levels in OVX rats, possibly via upregulation of renal 1-OHase expressions in renal proximal tubule cells. Our study indicates that FLL is a natural oral agent that could directly regulate renal vitamin D metabolism in vivo and in vitro. [ABSTRACT FROM AUTHOR] |
| Copyright of American Journal of Chinese Medicine is the property of World Scientific Publishing Company and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
|---|---|
| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 118832855 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Ethanol Extract of Fructus ligustri lucidi Increased Circulating 1,25(OH)<subscript>2</subscript>D<subscript>3</subscript> Levels, but Did Not Improve Calcium Balance in Mature Ovariectomized Rats. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Dong%2C+Xiao-Li%22">Dong, Xiao-Li</searchLink><br /><searchLink fieldCode="AR" term="%22Cao%2C+Si-Si%22">Cao, Si-Si</searchLink><br /><searchLink fieldCode="AR" term="%22Zhou%2C+Li-Ping%22">Zhou, Li-Ping</searchLink><br /><searchLink fieldCode="AR" term="%22Denney%2C+Liya%22">Denney, Liya</searchLink><br /><searchLink fieldCode="AR" term="%22Wong%2C+Man-Sau%22">Wong, Man-Sau</searchLink><br /><searchLink fieldCode="AR" term="%22Feng%2C+Hao-Tian%22">Feng, Hao-Tian</searchLink> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22American+Journal+of+Chinese+Medicine%22">American Journal of Chinese Medicine</searchLink>. 2016, Vol. 44 Issue 6, p1237-1253. 17p. 2 Charts, 5 Graphs. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Animal+experimentation%22">Animal experimentation</searchLink><br /><searchLink fieldCode="DE" term="%22Calcium%22">Calcium</searchLink><br /><searchLink fieldCode="DE" term="%22Diet%22">Diet</searchLink><br /><searchLink fieldCode="DE" term="%22Enzyme-linked+immunosorbent+assay%22">Enzyme-linked immunosorbent assay</searchLink><br /><searchLink fieldCode="DE" term="%22Polymerase+chain+reaction%22">Polymerase chain reaction</searchLink><br /><searchLink fieldCode="DE" term="%22Rats%22">Rats</searchLink><br /><searchLink fieldCode="DE" term="%22Research+funding%22">Research funding</searchLink><br /><searchLink fieldCode="DE" term="%22Statistics%22">Statistics</searchLink><br /><searchLink fieldCode="DE" term="%22T-test+%28Statistics%29%22">T-test (Statistics)</searchLink><br /><searchLink fieldCode="DE" term="%22Western+immunoblotting%22">Western immunoblotting</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+extracts%22">Plant extracts</searchLink><br /><searchLink fieldCode="DE" term="%22Data+analysis%22">Data analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Cholecalciferol%22">Cholecalciferol</searchLink><br /><searchLink fieldCode="DE" term="%22In+vitro+studies%22">In vitro studies</searchLink><br /><searchLink fieldCode="DE" term="%22One-way+analysis+of+variance%22">One-way analysis of variance</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Our previous studies found that different extracts or fractions of Fructus ligustri lucidi (FLL) played different roles in altering the regulation of bone and mineral metabolism in different animal models. The present study was designed to compare the actions of FLL ethanol (EE) and water extracts (WE) on bone and mineral metabolism in a 6-month-old mature ovariectomized (OVX) rat model. Our results showed that FLL extracts did not significantly improve systematic Ca balance in mature OVX rats. However, EE, but not WE treatment, significantly increased serum 1,25(OH)2D3 levels in mature OVX rats. An in vitro study using human proximal tubule (HKC-8) cells showed that EE, but not WE, significantly enhanced renal 25-dihydroxyvitamin D3-1-hydroxylase (1-OHase) mRNA expressions and simultaneously repressed renal 25-dihydroxyvitamin D3-24-hydroxylase (24-OHase) mRNA expressions. Further investigation indicated that EE could significantly induce the protein expression of 1-OHase, but did not alter 24-OHase expression in HKC-8 cells. Our results demonstrated that EE increased circulating 1,25(OH)2D3 levels in OVX rats, possibly via upregulation of renal 1-OHase expressions in renal proximal tubule cells. Our study indicates that FLL is a natural oral agent that could directly regulate renal vitamin D metabolism in vivo and in vitro. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of American Journal of Chinese Medicine is the property of World Scientific Publishing Company and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=118832855 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1142/S0192415X16500695 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 17 StartPage: 1237 Subjects: – SubjectFull: Animal experimentation Type: general – SubjectFull: Calcium Type: general – SubjectFull: Diet Type: general – SubjectFull: Enzyme-linked immunosorbent assay Type: general – SubjectFull: Polymerase chain reaction Type: general – SubjectFull: Rats Type: general – SubjectFull: Research funding Type: general – SubjectFull: Statistics Type: general – SubjectFull: T-test (Statistics) Type: general – SubjectFull: Western immunoblotting Type: general – SubjectFull: Plant extracts Type: general – SubjectFull: Data analysis Type: general – SubjectFull: Cholecalciferol Type: general – SubjectFull: In vitro studies Type: general – SubjectFull: One-way analysis of variance Type: general Titles: – TitleFull: Ethanol Extract of Fructus ligustri lucidi Increased Circulating 1,25(OH)2D3 Levels, but Did Not Improve Calcium Balance in Mature Ovariectomized Rats. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Dong, Xiao-Li – PersonEntity: Name: NameFull: Cao, Si-Si – PersonEntity: Name: NameFull: Zhou, Li-Ping – PersonEntity: Name: NameFull: Denney, Liya – PersonEntity: Name: NameFull: Wong, Man-Sau – PersonEntity: Name: NameFull: Feng, Hao-Tian IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 10 Text: 2016 Type: published Y: 2016 Identifiers: – Type: issn-print Value: 0192415X Numbering: – Type: volume Value: 44 – Type: issue Value: 6 Titles: – TitleFull: American Journal of Chinese Medicine Type: main |
| ResultId | 1 |
