View in EDS PDF Full Text

Antitumor and Immunomodulatory Effect of Gastrodia elata on Colon Cancer In Vitro and In Vivo.

Saved in:
Bibliographic Details
Title: Antitumor and Immunomodulatory Effect of Gastrodia elata on Colon Cancer In Vitro and In Vivo.
Authors: Kim, Na-Hyung, Xin, Ming Jie, Cha, Ji-Yoon, Ji, Soo-Jeong, Kwon, Se-Uk, Jee, Ho-Kyun, Park, Mi-Ran, Park, Yong-Soo, Kim, Chong-Tai, Kim, Dae-Ki, Lee, Young-Mi
Source: American Journal of Chinese Medicine. 2017, Vol. 45 Issue 2, p319-335. 17p. 1 Chart, 4 Graphs.
Subjects: Colon tumor prevention, Enzyme metabolism, Cell proliferation, Alternative medicine, Animal experimentation, Antineoplastic agents, Apoptosis, Biological models, Enzyme-linked immunosorbent assay, Flow cytometry, Immune system, Interferons, Interleukins, Liquid chromatography, Mathematics, Medicinal plants, Mice, Probability theory, Research funding, Spleen, Stains & staining (Microscopy), Statistics, Western immunoblotting, Plant extracts, Data analysis, Statistical significance, Data analysis software, Descriptive statistics, In vitro studies, One-way analysis of variance, In vivo studies, Pharmacodynamics
Abstract: Gastrodia elata Blume (GE) is a well-known kind of herb that has been used in traditional medicine for thousands of years. The extrusion of raw materials from it could improve flavor and enhance bioavailability in food and drug development. The purpose of this study is to investigate antitumor and immune boosting effects of extruded GE in human colon carcinoma cells, splenocytes, and mice-bearing CT26 colon carcinoma cell. Treatment with 100g/mL of extruded GE decreased cell viability and induced the expression of Caspase-3 and Bax in HT29 cells (). When we performed DAPI staining, apoptotic bodies with condensed chromatin and fragmented nuclei, known as indicative of apoptotic morphology, increased 24h after treatment with 100g/mL of extruded GE. Treatments with extruded GE significantly promoted splenocyte proliferation and IL-2 or IFN- secretion, compared with that of control cells (). The administration of extruded GE of 200 mg/kg/day decreased tumor growth and Ki-67 or -catenin expression in mice (). Additionally, we investigated the contents of compounds in extruded GE extracts using ultra performance liquid chromatography. The contents of p-hydroxylbenzyl alcohol and p-hydroxybenzaldehyde in extruded GE were 2.97mg/g and 0.04mg/g, respectively. It was supposed that antitumor and immunomodulatory effects of extruded GE might exert by the p-hydroxylbenzyl alcohol and p-hydroxybenzaldehyde of many compositions analyzed from extruded GE. These results suggest that extruded GE have the potential to be developed into a natural pharmaceutical and functional food as a cancer chemopreventive agent. [ABSTRACT FROM AUTHOR]
Copyright of American Journal of Chinese Medicine is the property of World Scientific Publishing Company and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
FullText Links:
  – Type: pdflink
Text:
  Availability: 0
Header DbId: pbh
DbLabel: Psychology and Behavioral Sciences Collection
An: 122570736
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Antitumor and Immunomodulatory Effect of Gastrodia elata on Colon Cancer In Vitro and In Vivo.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Kim%2C+Na-Hyung%22">Kim, Na-Hyung</searchLink><br /><searchLink fieldCode="AR" term="%22Xin%2C+Ming+Jie%22">Xin, Ming Jie</searchLink><br /><searchLink fieldCode="AR" term="%22Cha%2C+Ji-Yoon%22">Cha, Ji-Yoon</searchLink><br /><searchLink fieldCode="AR" term="%22Ji%2C+Soo-Jeong%22">Ji, Soo-Jeong</searchLink><br /><searchLink fieldCode="AR" term="%22Kwon%2C+Se-Uk%22">Kwon, Se-Uk</searchLink><br /><searchLink fieldCode="AR" term="%22Jee%2C+Ho-Kyun%22">Jee, Ho-Kyun</searchLink><br /><searchLink fieldCode="AR" term="%22Park%2C+Mi-Ran%22">Park, Mi-Ran</searchLink><br /><searchLink fieldCode="AR" term="%22Park%2C+Yong-Soo%22">Park, Yong-Soo</searchLink><br /><searchLink fieldCode="AR" term="%22Kim%2C+Chong-Tai%22">Kim, Chong-Tai</searchLink><br /><searchLink fieldCode="AR" term="%22Kim%2C+Dae-Ki%22">Kim, Dae-Ki</searchLink><br /><searchLink fieldCode="AR" term="%22Lee%2C+Young-Mi%22">Lee, Young-Mi</searchLink>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22American+Journal+of+Chinese+Medicine%22">American Journal of Chinese Medicine</searchLink>. 2017, Vol. 45 Issue 2, p319-335. 17p. 1 Chart, 4 Graphs.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Colon+tumor+prevention%22">Colon tumor prevention</searchLink><br /><searchLink fieldCode="DE" term="%22Enzyme+metabolism%22">Enzyme metabolism</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+proliferation%22">Cell proliferation</searchLink><br /><searchLink fieldCode="DE" term="%22Alternative+medicine%22">Alternative medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Animal+experimentation%22">Animal experimentation</searchLink><br /><searchLink fieldCode="DE" term="%22Antineoplastic+agents%22">Antineoplastic agents</searchLink><br /><searchLink fieldCode="DE" term="%22Apoptosis%22">Apoptosis</searchLink><br /><searchLink fieldCode="DE" term="%22Biological+models%22">Biological models</searchLink><br /><searchLink fieldCode="DE" term="%22Enzyme-linked+immunosorbent+assay%22">Enzyme-linked immunosorbent assay</searchLink><br /><searchLink fieldCode="DE" term="%22Flow+cytometry%22">Flow cytometry</searchLink><br /><searchLink fieldCode="DE" term="%22Immune+system%22">Immune system</searchLink><br /><searchLink fieldCode="DE" term="%22Interferons%22">Interferons</searchLink><br /><searchLink fieldCode="DE" term="%22Interleukins%22">Interleukins</searchLink><br /><searchLink fieldCode="DE" term="%22Liquid+chromatography%22">Liquid chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22Mathematics%22">Mathematics</searchLink><br /><searchLink fieldCode="DE" term="%22Medicinal+plants%22">Medicinal plants</searchLink><br /><searchLink fieldCode="DE" term="%22Mice%22">Mice</searchLink><br /><searchLink fieldCode="DE" term="%22Probability+theory%22">Probability theory</searchLink><br /><searchLink fieldCode="DE" term="%22Research+funding%22">Research funding</searchLink><br /><searchLink fieldCode="DE" term="%22Spleen%22">Spleen</searchLink><br /><searchLink fieldCode="DE" term="%22Stains+%26+staining+%28Microscopy%29%22">Stains & staining (Microscopy)</searchLink><br /><searchLink fieldCode="DE" term="%22Statistics%22">Statistics</searchLink><br /><searchLink fieldCode="DE" term="%22Western+immunoblotting%22">Western immunoblotting</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+extracts%22">Plant extracts</searchLink><br /><searchLink fieldCode="DE" term="%22Data+analysis%22">Data analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Statistical+significance%22">Statistical significance</searchLink><br /><searchLink fieldCode="DE" term="%22Data+analysis+software%22">Data analysis software</searchLink><br /><searchLink fieldCode="DE" term="%22Descriptive+statistics%22">Descriptive statistics</searchLink><br /><searchLink fieldCode="DE" term="%22In+vitro+studies%22">In vitro studies</searchLink><br /><searchLink fieldCode="DE" term="%22One-way+analysis+of+variance%22">One-way analysis of variance</searchLink><br /><searchLink fieldCode="DE" term="%22In+vivo+studies%22">In vivo studies</searchLink><br /><searchLink fieldCode="DE" term="%22Pharmacodynamics%22">Pharmacodynamics</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Gastrodia elata Blume (GE) is a well-known kind of herb that has been used in traditional medicine for thousands of years. The extrusion of raw materials from it could improve flavor and enhance bioavailability in food and drug development. The purpose of this study is to investigate antitumor and immune boosting effects of extruded GE in human colon carcinoma cells, splenocytes, and mice-bearing CT26 colon carcinoma cell. Treatment with 100g/mL of extruded GE decreased cell viability and induced the expression of Caspase-3 and Bax in HT29 cells (). When we performed DAPI staining, apoptotic bodies with condensed chromatin and fragmented nuclei, known as indicative of apoptotic morphology, increased 24h after treatment with 100g/mL of extruded GE. Treatments with extruded GE significantly promoted splenocyte proliferation and IL-2 or IFN- secretion, compared with that of control cells (). The administration of extruded GE of 200 mg/kg/day decreased tumor growth and Ki-67 or -catenin expression in mice (). Additionally, we investigated the contents of compounds in extruded GE extracts using ultra performance liquid chromatography. The contents of p-hydroxylbenzyl alcohol and p-hydroxybenzaldehyde in extruded GE were 2.97mg/g and 0.04mg/g, respectively. It was supposed that antitumor and immunomodulatory effects of extruded GE might exert by the p-hydroxylbenzyl alcohol and p-hydroxybenzaldehyde of many compositions analyzed from extruded GE. These results suggest that extruded GE have the potential to be developed into a natural pharmaceutical and functional food as a cancer chemopreventive agent. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of American Journal of Chinese Medicine is the property of World Scientific Publishing Company and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=122570736
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1142/S0192415X17500203
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 17
        StartPage: 319
    Subjects:
      – SubjectFull: Colon tumor prevention
        Type: general
      – SubjectFull: Enzyme metabolism
        Type: general
      – SubjectFull: Cell proliferation
        Type: general
      – SubjectFull: Alternative medicine
        Type: general
      – SubjectFull: Animal experimentation
        Type: general
      – SubjectFull: Antineoplastic agents
        Type: general
      – SubjectFull: Apoptosis
        Type: general
      – SubjectFull: Biological models
        Type: general
      – SubjectFull: Enzyme-linked immunosorbent assay
        Type: general
      – SubjectFull: Flow cytometry
        Type: general
      – SubjectFull: Immune system
        Type: general
      – SubjectFull: Interferons
        Type: general
      – SubjectFull: Interleukins
        Type: general
      – SubjectFull: Liquid chromatography
        Type: general
      – SubjectFull: Mathematics
        Type: general
      – SubjectFull: Medicinal plants
        Type: general
      – SubjectFull: Mice
        Type: general
      – SubjectFull: Probability theory
        Type: general
      – SubjectFull: Research funding
        Type: general
      – SubjectFull: Spleen
        Type: general
      – SubjectFull: Stains & staining (Microscopy)
        Type: general
      – SubjectFull: Statistics
        Type: general
      – SubjectFull: Western immunoblotting
        Type: general
      – SubjectFull: Plant extracts
        Type: general
      – SubjectFull: Data analysis
        Type: general
      – SubjectFull: Statistical significance
        Type: general
      – SubjectFull: Data analysis software
        Type: general
      – SubjectFull: Descriptive statistics
        Type: general
      – SubjectFull: In vitro studies
        Type: general
      – SubjectFull: One-way analysis of variance
        Type: general
      – SubjectFull: In vivo studies
        Type: general
      – SubjectFull: Pharmacodynamics
        Type: general
    Titles:
      – TitleFull: Antitumor and Immunomodulatory Effect of Gastrodia elata on Colon Cancer In Vitro and In Vivo.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Kim, Na-Hyung
      – PersonEntity:
          Name:
            NameFull: Xin, Ming Jie
      – PersonEntity:
          Name:
            NameFull: Cha, Ji-Yoon
      – PersonEntity:
          Name:
            NameFull: Ji, Soo-Jeong
      – PersonEntity:
          Name:
            NameFull: Kwon, Se-Uk
      – PersonEntity:
          Name:
            NameFull: Jee, Ho-Kyun
      – PersonEntity:
          Name:
            NameFull: Park, Mi-Ran
      – PersonEntity:
          Name:
            NameFull: Park, Yong-Soo
      – PersonEntity:
          Name:
            NameFull: Kim, Chong-Tai
      – PersonEntity:
          Name:
            NameFull: Kim, Dae-Ki
      – PersonEntity:
          Name:
            NameFull: Lee, Young-Mi
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 03
              Text: 2017
              Type: published
              Y: 2017
          Identifiers:
            – Type: issn-print
              Value: 0192415X
          Numbering:
            – Type: volume
              Value: 45
            – Type: issue
              Value: 2
          Titles:
            – TitleFull: American Journal of Chinese Medicine
              Type: main
ResultId 1