Expressional profile of the diacylglycerol kinase eta gene DGKH.
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| Title: | Expressional profile of the diacylglycerol kinase eta gene DGKH. |
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| Authors: | Weißflog, Lena, Becker, Nils, Bossert, Nelli, Freudenberg, Florian, Kittel-Schneider, Sarah, Reif, Andreas |
| Source: | European Archives of Psychiatry & Clinical Neuroscience. Aug2017, Vol. 267 Issue 5, p445-454. 10p. 1 Color Photograph, 4 Diagrams, 2 Graphs. |
| Subjects: | Gene expression, Diglycerides, Bipolar disorder, Neural development, Phosphatidylinositol 3-kinases, Polymerase chain reaction |
| Abstract: | Bipolar disorder (BPD) is a genetically complex mental disorder, which is characterized by recurrent depressive and manic episodes, occurring with a typical cyclical course. In a recent study, we were able to identify a risk haplotype for BPD, as well as for unipolar depression and adult attention-deficit/hyperactivity disorder (ADHD), within the DGKH gene. DGKH codes for the eta (η) isoform of diacylglycerol kinase, which is involved in the phosphoinositol pathway. In the present study, we determined the expressional profile of Dgkh using quantitative real-time PCR (qPCR), in situ hybridization and immunohistological staining in the human and in the mouse brain. Expression studies showed that two different Dgkh transcripts exhibited distinct occurrence in a variety of murine tissues and also differed in their expression levels. The proteins encoded by those transcripts differ in functional protein domains suggesting distinct biochemical and cell biological properties and functions. qPCR analyses revealed an increase in Dgkh expression during mouse brain development indicating a possible role of this kinase in late developmental stages. Immunostainings revealed strong Dgkh expression in neurons of the hippocampus and the cerebellum of the murine brain, whereas highest expression levels of DGKH in the human brain were found in the striatum. Taken together, our studies revealed expressional changes during mouse brain development and occurrence of Dgkη in neurons of regions that have been linked to BPD as well as ADHD in humans providing evidence for the implication of DGKH in those disorders. [ABSTRACT FROM AUTHOR] |
| Copyright of European Archives of Psychiatry & Clinical Neuroscience is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 124090339 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Expressional profile of the diacylglycerol kinase eta gene DGKH. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Weißflog%2C+Lena%22">Weißflog, Lena</searchLink><br /><searchLink fieldCode="AR" term="%22Becker%2C+Nils%22">Becker, Nils</searchLink><br /><searchLink fieldCode="AR" term="%22Bossert%2C+Nelli%22">Bossert, Nelli</searchLink><br /><searchLink fieldCode="AR" term="%22Freudenberg%2C+Florian%22">Freudenberg, Florian</searchLink><br /><searchLink fieldCode="AR" term="%22Kittel-Schneider%2C+Sarah%22">Kittel-Schneider, Sarah</searchLink><br /><searchLink fieldCode="AR" term="%22Reif%2C+Andreas%22">Reif, Andreas</searchLink> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22European+Archives+of+Psychiatry+%26+Clinical+Neuroscience%22">European Archives of Psychiatry & Clinical Neuroscience</searchLink>. Aug2017, Vol. 267 Issue 5, p445-454. 10p. 1 Color Photograph, 4 Diagrams, 2 Graphs. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink><br /><searchLink fieldCode="DE" term="%22Diglycerides%22">Diglycerides</searchLink><br /><searchLink fieldCode="DE" term="%22Bipolar+disorder%22">Bipolar disorder</searchLink><br /><searchLink fieldCode="DE" term="%22Neural+development%22">Neural development</searchLink><br /><searchLink fieldCode="DE" term="%22Phosphatidylinositol+3-kinases%22">Phosphatidylinositol 3-kinases</searchLink><br /><searchLink fieldCode="DE" term="%22Polymerase+chain+reaction%22">Polymerase chain reaction</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Bipolar disorder (BPD) is a genetically complex mental disorder, which is characterized by recurrent depressive and manic episodes, occurring with a typical cyclical course. In a recent study, we were able to identify a risk haplotype for BPD, as well as for unipolar depression and adult attention-deficit/hyperactivity disorder (ADHD), within the DGKH gene. DGKH codes for the eta (η) isoform of diacylglycerol kinase, which is involved in the phosphoinositol pathway. In the present study, we determined the expressional profile of Dgkh using quantitative real-time PCR (qPCR), in situ hybridization and immunohistological staining in the human and in the mouse brain. Expression studies showed that two different Dgkh transcripts exhibited distinct occurrence in a variety of murine tissues and also differed in their expression levels. The proteins encoded by those transcripts differ in functional protein domains suggesting distinct biochemical and cell biological properties and functions. qPCR analyses revealed an increase in Dgkh expression during mouse brain development indicating a possible role of this kinase in late developmental stages. Immunostainings revealed strong Dgkh expression in neurons of the hippocampus and the cerebellum of the murine brain, whereas highest expression levels of DGKH in the human brain were found in the striatum. Taken together, our studies revealed expressional changes during mouse brain development and occurrence of Dgkη in neurons of regions that have been linked to BPD as well as ADHD in humans providing evidence for the implication of DGKH in those disorders. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of European Archives of Psychiatry & Clinical Neuroscience is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00406-016-0695-4 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 445 Subjects: – SubjectFull: Gene expression Type: general – SubjectFull: Diglycerides Type: general – SubjectFull: Bipolar disorder Type: general – SubjectFull: Neural development Type: general – SubjectFull: Phosphatidylinositol 3-kinases Type: general – SubjectFull: Polymerase chain reaction Type: general Titles: – TitleFull: Expressional profile of the diacylglycerol kinase eta gene DGKH. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Weißflog, Lena – PersonEntity: Name: NameFull: Becker, Nils – PersonEntity: Name: NameFull: Bossert, Nelli – PersonEntity: Name: NameFull: Freudenberg, Florian – PersonEntity: Name: NameFull: Kittel-Schneider, Sarah – PersonEntity: Name: NameFull: Reif, Andreas IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 08 Text: Aug2017 Type: published Y: 2017 Identifiers: – Type: issn-print Value: 09401334 Numbering: – Type: volume Value: 267 – Type: issue Value: 5 Titles: – TitleFull: European Archives of Psychiatry & Clinical Neuroscience Type: main |
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