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Cerebrospinal fluid cytotoxicity does not affect survival in amyotrophic lateral sclerosis.

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Title: Cerebrospinal fluid cytotoxicity does not affect survival in amyotrophic lateral sclerosis.
Authors: Galán, L., Matías‐Guiu, J., Matias‐Guiu, J. A., Yáñez, M., Pytel, V., Guerrero‐Sola, A., Vela‐Souto, A., Arranz‐Tagarro, J. A., Gómez‐Pinedo, U., García, A. G.
Source: Acta Neurologica Scandinavica. Sep2017, Vol. 136 Issue 3, p212-216. 5p.
Subjects: Amyotrophic lateral sclerosis, Cell-mediated cytotoxicity, Cerebrospinal fluid, Motor neurons, Patients, Primary cell culture
Abstract: Objectives Cerebrospinal fluid ( CSF) from some patients with amyotrophic lateral sclerosis ( ALS) has been demonstrated to significantly reduce the neuronal viability of primary cell cultures of motor neurons. We aimed to study the potential clinical consequences associated with the cytotoxicity of CSF in a cohort of patients with ALS. Methods We collected CSF from thirty-one patients with ALS. We analysed cytotoxicity by incubating it into the primary cultures of motor cortex neurons. Neural viability was quantified after 24 hours using the colorimetric MTT reduction assay. All patients were followed up from the moment of diagnosis to death, and a complete evaluation during disease progression and survival was performed, including gastrostomy and respiratory assistance. Results Twenty-one patients (67.7%) presented a cytotoxic CSF. There were no significant differences between patients with and without cytotoxicity regarding mean time from symptom onset to the diagnosis, from the diagnosis to death, from the diagnosis to respiratory assistance with BIPAP, from diagnosis to gastrostomy and from the onset of symptoms to death. In Cox regression analysis, bulbar onset, but not cytotoxicity, gender or age at onset, was associated with a lower risk of survival. Conclusions Cerebrospinal fluid cytotoxicity was not associated with differential survival rates. This suggests that the presence of cytotoxicity in CSF, measured through neuronal viability in primary cultures of motor cortex neurons, could reflect different mechanisms of the disease, but it does not predict disease outcome. [ABSTRACT FROM AUTHOR]
Copyright of Acta Neurologica Scandinavica is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Data: Cerebrospinal fluid cytotoxicity does not affect survival in amyotrophic lateral sclerosis.
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  Data: <searchLink fieldCode="AR" term="%22Galán%2C+L%2E%22">Galán, L.</searchLink><br /><searchLink fieldCode="AR" term="%22Matías‐Guiu%2C+J%2E%22">Matías‐Guiu, J.</searchLink><br /><searchLink fieldCode="AR" term="%22Matias‐Guiu%2C+J%2E+A%2E%22">Matias‐Guiu, J. A.</searchLink><br /><searchLink fieldCode="AR" term="%22Yáñez%2C+M%2E%22">Yáñez, M.</searchLink><br /><searchLink fieldCode="AR" term="%22Pytel%2C+V%2E%22">Pytel, V.</searchLink><br /><searchLink fieldCode="AR" term="%22Guerrero‐Sola%2C+A%2E%22">Guerrero‐Sola, A.</searchLink><br /><searchLink fieldCode="AR" term="%22Vela‐Souto%2C+A%2E%22">Vela‐Souto, A.</searchLink><br /><searchLink fieldCode="AR" term="%22Arranz‐Tagarro%2C+J%2E+A%2E%22">Arranz‐Tagarro, J. A.</searchLink><br /><searchLink fieldCode="AR" term="%22Gómez‐Pinedo%2C+U%2E%22">Gómez‐Pinedo, U.</searchLink><br /><searchLink fieldCode="AR" term="%22García%2C+A%2E+G%2E%22">García, A. G.</searchLink>
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  Data: <searchLink fieldCode="JN" term="%22Acta+Neurologica+Scandinavica%22">Acta Neurologica Scandinavica</searchLink>. Sep2017, Vol. 136 Issue 3, p212-216. 5p.
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  Data: <searchLink fieldCode="DE" term="%22Amyotrophic+lateral+sclerosis%22">Amyotrophic lateral sclerosis</searchLink><br /><searchLink fieldCode="DE" term="%22Cell-mediated+cytotoxicity%22">Cell-mediated cytotoxicity</searchLink><br /><searchLink fieldCode="DE" term="%22Cerebrospinal+fluid%22">Cerebrospinal fluid</searchLink><br /><searchLink fieldCode="DE" term="%22Motor+neurons%22">Motor neurons</searchLink><br /><searchLink fieldCode="DE" term="%22Patients%22">Patients</searchLink><br /><searchLink fieldCode="DE" term="%22Primary+cell+culture%22">Primary cell culture</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Objectives Cerebrospinal fluid ( CSF) from some patients with amyotrophic lateral sclerosis ( ALS) has been demonstrated to significantly reduce the neuronal viability of primary cell cultures of motor neurons. We aimed to study the potential clinical consequences associated with the cytotoxicity of CSF in a cohort of patients with ALS. Methods We collected CSF from thirty-one patients with ALS. We analysed cytotoxicity by incubating it into the primary cultures of motor cortex neurons. Neural viability was quantified after 24 hours using the colorimetric MTT reduction assay. All patients were followed up from the moment of diagnosis to death, and a complete evaluation during disease progression and survival was performed, including gastrostomy and respiratory assistance. Results Twenty-one patients (67.7%) presented a cytotoxic CSF. There were no significant differences between patients with and without cytotoxicity regarding mean time from symptom onset to the diagnosis, from the diagnosis to death, from the diagnosis to respiratory assistance with BIPAP, from diagnosis to gastrostomy and from the onset of symptoms to death. In Cox regression analysis, bulbar onset, but not cytotoxicity, gender or age at onset, was associated with a lower risk of survival. Conclusions Cerebrospinal fluid cytotoxicity was not associated with differential survival rates. This suggests that the presence of cytotoxicity in CSF, measured through neuronal viability in primary cultures of motor cortex neurons, could reflect different mechanisms of the disease, but it does not predict disease outcome. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
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  Data: <i>Copyright of Acta Neurologica Scandinavica is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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