Impaired butyrate absorption in the proximal colon, low serum butyrate and diminished central effects of butyrate on blood pressure in spontaneously hypertensive rats.
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| Title: | Impaired butyrate absorption in the proximal colon, low serum butyrate and diminished central effects of butyrate on blood pressure in spontaneously hypertensive rats. |
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| Authors: | Yang, Tao, Magee, Kacy L., Colon‐Perez, Luis M., Larkin, Riley, Liao, Yan‐Shin, Balazic, Eliza, Cowart, Jonathan R., Arocha, Rebeca, Redler, Ty, Febo, Marcelo, Vickroy, Thomas, Martyniuk, Christopher J., Reznikov, Leah R., Zubcevic, Jasenka |
| Source: | Acta Physiologica. Jun2019, Vol. 226 Issue 2, pN.PAG-N.PAG. 1p. 1 Color Photograph, 1 Chart, 4 Graphs. |
| Subjects: | Blood pressure, Butyrates, Functional magnetic resonance imaging, Liquid chromatography-mass spectrometry, Regulation of blood pressure |
| Abstract: | Aim: Butyrate is a major gut microbiota‐derived metabolite. Reduced butyrate‐producing bacteria has been reported in the spontaneously hypertensive rat (SHR), a model of hypertension characterized by dysfunctional autonomic nervous system and gut dysbiosis. Here, we demonstrate a potential mechanism for butyrate in blood pressure regulation. Methods: High‐performance liquid chromatography and liquid chromatography‐mass spectrometry were performed to measure butyrate levels in feces and serum. Ussing chamber determined butyrate transport in colon ex vivo. Real‐time PCR and immunohistochemistry evaluated expression of butyrate transporter, Slc5a8, in the colon. Mean arterial blood pressure was measured in catheterized anesthetized rats before and after a single butyrate intracerebroventricular injection. Activity of cardioregulatory brain regions was determined by functional magnetic resonance imaging to derive neural effects of butyrate. Results: In the SHR, we demonstrated elevated butyrate levels in cecal content, but diminished butyrate levels in circulation, possibly due to reduced expression of Slc5a8 transporter in the colon. In addition, we observed lower expression levels of butyrate‐sensing receptors in the hypothalamus of SHR, likely leading to the reduced effects of centrally administered butyrate on blood pressure in the SHR. Functional magnetic resonance imaging revealed reduced activation of cardioregulatory brain regions following central administration of butyrate in the SHR compared to control. Conclusion: We demonstrated a reduced availability of serum butyrate in the SHR, possibly due to diminished colonic absorption. Reduced expression of butyrate‐sensing receptors in the SHR hypothalamus may explain the reduced central responsiveness to butyrate, indicating microbial butyrate may play a role in blood pressure regulation. [ABSTRACT FROM AUTHOR] |
| Copyright of Acta Physiologica is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
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| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 136269841 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Impaired butyrate absorption in the proximal colon, low serum butyrate and diminished central effects of butyrate on blood pressure in spontaneously hypertensive rats. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Yang%2C+Tao%22">Yang, Tao</searchLink><br /><searchLink fieldCode="AR" term="%22Magee%2C+Kacy+L%2E%22">Magee, Kacy L.</searchLink><br /><searchLink fieldCode="AR" term="%22Colon‐Perez%2C+Luis+M%2E%22">Colon‐Perez, Luis M.</searchLink><br /><searchLink fieldCode="AR" term="%22Larkin%2C+Riley%22">Larkin, Riley</searchLink><br /><searchLink fieldCode="AR" term="%22Liao%2C+Yan‐Shin%22">Liao, Yan‐Shin</searchLink><br /><searchLink fieldCode="AR" term="%22Balazic%2C+Eliza%22">Balazic, Eliza</searchLink><br /><searchLink fieldCode="AR" term="%22Cowart%2C+Jonathan+R%2E%22">Cowart, Jonathan R.</searchLink><br /><searchLink fieldCode="AR" term="%22Arocha%2C+Rebeca%22">Arocha, Rebeca</searchLink><br /><searchLink fieldCode="AR" term="%22Redler%2C+Ty%22">Redler, Ty</searchLink><br /><searchLink fieldCode="AR" term="%22Febo%2C+Marcelo%22">Febo, Marcelo</searchLink><br /><searchLink fieldCode="AR" term="%22Vickroy%2C+Thomas%22">Vickroy, Thomas</searchLink><br /><searchLink fieldCode="AR" term="%22Martyniuk%2C+Christopher+J%2E%22">Martyniuk, Christopher J.</searchLink><br /><searchLink fieldCode="AR" term="%22Reznikov%2C+Leah+R%2E%22">Reznikov, Leah R.</searchLink><br /><searchLink fieldCode="AR" term="%22Zubcevic%2C+Jasenka%22">Zubcevic, Jasenka</searchLink> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Acta+Physiologica%22">Acta Physiologica</searchLink>. Jun2019, Vol. 226 Issue 2, pN.PAG-N.PAG. 1p. 1 Color Photograph, 1 Chart, 4 Graphs. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Blood+pressure%22">Blood pressure</searchLink><br /><searchLink fieldCode="DE" term="%22Butyrates%22">Butyrates</searchLink><br /><searchLink fieldCode="DE" term="%22Functional+magnetic+resonance+imaging%22">Functional magnetic resonance imaging</searchLink><br /><searchLink fieldCode="DE" term="%22Liquid+chromatography-mass+spectrometry%22">Liquid chromatography-mass spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Regulation+of+blood+pressure%22">Regulation of blood pressure</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Aim: Butyrate is a major gut microbiota‐derived metabolite. Reduced butyrate‐producing bacteria has been reported in the spontaneously hypertensive rat (SHR), a model of hypertension characterized by dysfunctional autonomic nervous system and gut dysbiosis. Here, we demonstrate a potential mechanism for butyrate in blood pressure regulation. Methods: High‐performance liquid chromatography and liquid chromatography‐mass spectrometry were performed to measure butyrate levels in feces and serum. Ussing chamber determined butyrate transport in colon ex vivo. Real‐time PCR and immunohistochemistry evaluated expression of butyrate transporter, Slc5a8, in the colon. Mean arterial blood pressure was measured in catheterized anesthetized rats before and after a single butyrate intracerebroventricular injection. Activity of cardioregulatory brain regions was determined by functional magnetic resonance imaging to derive neural effects of butyrate. Results: In the SHR, we demonstrated elevated butyrate levels in cecal content, but diminished butyrate levels in circulation, possibly due to reduced expression of Slc5a8 transporter in the colon. In addition, we observed lower expression levels of butyrate‐sensing receptors in the hypothalamus of SHR, likely leading to the reduced effects of centrally administered butyrate on blood pressure in the SHR. Functional magnetic resonance imaging revealed reduced activation of cardioregulatory brain regions following central administration of butyrate in the SHR compared to control. Conclusion: We demonstrated a reduced availability of serum butyrate in the SHR, possibly due to diminished colonic absorption. Reduced expression of butyrate‐sensing receptors in the SHR hypothalamus may explain the reduced central responsiveness to butyrate, indicating microbial butyrate may play a role in blood pressure regulation. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Acta Physiologica is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=136269841 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1111/apha.13256 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 1 StartPage: N.PAG Subjects: – SubjectFull: Blood pressure Type: general – SubjectFull: Butyrates Type: general – SubjectFull: Functional magnetic resonance imaging Type: general – SubjectFull: Liquid chromatography-mass spectrometry Type: general – SubjectFull: Regulation of blood pressure Type: general Titles: – TitleFull: Impaired butyrate absorption in the proximal colon, low serum butyrate and diminished central effects of butyrate on blood pressure in spontaneously hypertensive rats. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Yang, Tao – PersonEntity: Name: NameFull: Magee, Kacy L. – PersonEntity: Name: NameFull: Colon‐Perez, Luis M. – PersonEntity: Name: NameFull: Larkin, Riley – PersonEntity: Name: NameFull: Liao, Yan‐Shin – PersonEntity: Name: NameFull: Balazic, Eliza – PersonEntity: Name: NameFull: Cowart, Jonathan R. – PersonEntity: Name: NameFull: Arocha, Rebeca – PersonEntity: Name: NameFull: Redler, Ty – PersonEntity: Name: NameFull: Febo, Marcelo – PersonEntity: Name: NameFull: Vickroy, Thomas – PersonEntity: Name: NameFull: Martyniuk, Christopher J. – PersonEntity: Name: NameFull: Reznikov, Leah R. – PersonEntity: Name: NameFull: Zubcevic, Jasenka IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 06 Text: Jun2019 Type: published Y: 2019 Identifiers: – Type: issn-print Value: 17481708 Numbering: – Type: volume Value: 226 – Type: issue Value: 2 Titles: – TitleFull: Acta Physiologica Type: main |
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