View in EDS HTML Full Text PDF Full Text

Identification of a regeneration-organizing cell in the Xenopus tail.

Saved in:
Bibliographic Details
Title: Identification of a regeneration-organizing cell in the Xenopus tail.
Authors: Aztekin, C. (AUTHOR), Hiscock, T. W. (AUTHOR), Marioni, J. C. (AUTHOR), Gurdon, J. B. (AUTHOR), Simons, B. D. (AUTHOR), Jullien, J. (AUTHOR)
Source: Science (pre-March 2025). 5/17/2019, Vol. 364 Issue 6441, p653-658. 6p. 6 Diagrams.
Subjects: Xenopus, Limb regeneration, Tadpoles, Ligands (Biochemistry), Cellular mechanics, Epidermis
Abstract: Unlike mammals, Xenopus laevis tadpoles have a high regenerative potential. To characterize this regenerative response, we performed single-cell RNA sequencing after tail amputation. By comparing naturally occurring regeneration-competent and -incompetent tadpoles, we identified a previously unrecognized cell type, which we term the regeneration-organizing cell (ROC). ROCs are present in the epidermis during normal tail development and specifically relocalize to the amputation plane of regeneration-competent tadpoles, forming the wound epidermis. Genetic ablation or manual removal of ROCs blocks regeneration, whereas transplantation of ROC-containing grafts induces ectopic outgrowths in early embryos. Transcriptional profiling revealed that ROCs secrete ligands associated with key regenerative pathways, signaling to progenitors to reconstitute lost tissue. These findings reveal the cellular mechanism through which ROCs form the wound epidermis and ensure successful regeneration. [ABSTRACT FROM AUTHOR]
Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
Full text is not displayed to guests.
FullText Links:
  – Type: pdflink
Text:
  Availability: 1
Header DbId: pbh
DbLabel: Psychology and Behavioral Sciences Collection
An: 136517772
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Identification of a regeneration-organizing cell in the Xenopus tail.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Aztekin%2C+C%2E%22">Aztekin, C.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hiscock%2C+T%2E+W%2E%22">Hiscock, T. W.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Marioni%2C+J%2E+C%2E%22">Marioni, J. C.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Gurdon%2C+J%2E+B%2E%22">Gurdon, J. B.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Simons%2C+B%2E+D%2E%22">Simons, B. D.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Jullien%2C+J%2E%22">Jullien, J.</searchLink> (AUTHOR)
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Science+%28pre-March+2025%29%22">Science (pre-March 2025)</searchLink>. 5/17/2019, Vol. 364 Issue 6441, p653-658. 6p. 6 Diagrams.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Xenopus%22">Xenopus</searchLink><br /><searchLink fieldCode="DE" term="%22Limb+regeneration%22">Limb regeneration</searchLink><br /><searchLink fieldCode="DE" term="%22Tadpoles%22">Tadpoles</searchLink><br /><searchLink fieldCode="DE" term="%22Ligands+%28Biochemistry%29%22">Ligands (Biochemistry)</searchLink><br /><searchLink fieldCode="DE" term="%22Cellular+mechanics%22">Cellular mechanics</searchLink><br /><searchLink fieldCode="DE" term="%22Epidermis%22">Epidermis</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Unlike mammals, Xenopus laevis tadpoles have a high regenerative potential. To characterize this regenerative response, we performed single-cell RNA sequencing after tail amputation. By comparing naturally occurring regeneration-competent and -incompetent tadpoles, we identified a previously unrecognized cell type, which we term the regeneration-organizing cell (ROC). ROCs are present in the epidermis during normal tail development and specifically relocalize to the amputation plane of regeneration-competent tadpoles, forming the wound epidermis. Genetic ablation or manual removal of ROCs blocks regeneration, whereas transplantation of ROC-containing grafts induces ectopic outgrowths in early embryos. Transcriptional profiling revealed that ROCs secrete ligands associated with key regenerative pathways, signaling to progenitors to reconstitute lost tissue. These findings reveal the cellular mechanism through which ROCs form the wound epidermis and ensure successful regeneration. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=136517772
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1126/science.aav9996
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 6
        StartPage: 653
    Subjects:
      – SubjectFull: Xenopus
        Type: general
      – SubjectFull: Limb regeneration
        Type: general
      – SubjectFull: Tadpoles
        Type: general
      – SubjectFull: Ligands (Biochemistry)
        Type: general
      – SubjectFull: Cellular mechanics
        Type: general
      – SubjectFull: Epidermis
        Type: general
    Titles:
      – TitleFull: Identification of a regeneration-organizing cell in the Xenopus tail.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Aztekin, C.
      – PersonEntity:
          Name:
            NameFull: Hiscock, T. W.
      – PersonEntity:
          Name:
            NameFull: Marioni, J. C.
      – PersonEntity:
          Name:
            NameFull: Gurdon, J. B.
      – PersonEntity:
          Name:
            NameFull: Simons, B. D.
      – PersonEntity:
          Name:
            NameFull: Jullien, J.
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 17
              M: 05
              Text: 5/17/2019
              Type: published
              Y: 2019
          Identifiers:
            – Type: issn-print
              Value: 00368075
          Numbering:
            – Type: volume
              Value: 364
            – Type: issue
              Value: 6441
          Titles:
            – TitleFull: Science (pre-March 2025)
              Type: main
ResultId 1