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Isolation and receptor profiling of ileal enterochromaffin cells.

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Title: Isolation and receptor profiling of ileal enterochromaffin cells.
Authors: Schäfermeyer, A., Gratzl, M., Rad, R., Dossumbekova, A., Sachs, G., Prinz, C.
Source: Acta Physiologica Scandinavica. Sep2004, Vol. 182 Issue 1, p53-62. 10p.
Subjects: Gastrointestinal mucosa, Serotonin, Cells, Muscarinic receptors, Noradrenaline, Gene expression
Abstract: Enterochromaffin (EC) cells, interspersed throughout the gastrointestinal mucosa, provide most of the serotonin of the body and control intestinal motility, secretion and absorption. We purified EC cells from the rat ileum by a combination of elutriation and density gradient centrifugation in order to characterize the function of this important cell type. Immunostaining showed that there were 84% serotonin-positive cells in the highly enriched EC fraction as compared with 12% in unfractionated cells, yielding a approximately sevenfold enrichment. Serotonin measurements in the cell suspensions indicated a seven to 14-fold enrichment. Presence of alpha- and beta-adrenoreceptor isoforms, muscarinic M3 and gama-aminobutyric acid (GABA)-A receptors was confirmed by RT-PCR and cytochemistry. Increased expression of VMAT-1 and GABA-A mRNA was also shown by quantitative TaqMan PCR using EC cell RNA. Serotonin release in isolated EC cells was stimulated by noradrenaline, and to a smaller extent, by carbachol, while GABA addition was without effect. Our data provide a basis for a new approach to characterize receptors on this unique cell type. [ABSTRACT FROM AUTHOR]
Copyright of Acta Physiologica Scandinavica is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Data: Isolation and receptor profiling of ileal enterochromaffin cells.
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  Data: <searchLink fieldCode="DE" term="%22Gastrointestinal+mucosa%22">Gastrointestinal mucosa</searchLink><br /><searchLink fieldCode="DE" term="%22Serotonin%22">Serotonin</searchLink><br /><searchLink fieldCode="DE" term="%22Cells%22">Cells</searchLink><br /><searchLink fieldCode="DE" term="%22Muscarinic+receptors%22">Muscarinic receptors</searchLink><br /><searchLink fieldCode="DE" term="%22Noradrenaline%22">Noradrenaline</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink>
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  Data: Enterochromaffin (EC) cells, interspersed throughout the gastrointestinal mucosa, provide most of the serotonin of the body and control intestinal motility, secretion and absorption. We purified EC cells from the rat ileum by a combination of elutriation and density gradient centrifugation in order to characterize the function of this important cell type. Immunostaining showed that there were 84% serotonin-positive cells in the highly enriched EC fraction as compared with 12% in unfractionated cells, yielding a approximately sevenfold enrichment. Serotonin measurements in the cell suspensions indicated a seven to 14-fold enrichment. Presence of alpha- and beta-adrenoreceptor isoforms, muscarinic M3 and gama-aminobutyric acid (GABA)-A receptors was confirmed by RT-PCR and cytochemistry. Increased expression of VMAT-1 and GABA-A mRNA was also shown by quantitative TaqMan PCR using EC cell RNA. Serotonin release in isolated EC cells was stimulated by noradrenaline, and to a smaller extent, by carbachol, while GABA addition was without effect. Our data provide a basis for a new approach to characterize receptors on this unique cell type. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Acta Physiologica Scandinavica is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1111/j.1365-201X.2004.01299.x
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        Text: English
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        Type: general
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      – SubjectFull: Cells
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