Dynamic changes of the anti- and pro-apoptotic proteins Bcl-w, Bcl-2, and Bax with Smac/Diablo mitochondrial release after photothrombotic ring stroke in rats.
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| Title: | Dynamic changes of the anti- and pro-apoptotic proteins Bcl-w, Bcl-2, and Bax with Smac/Diablo mitochondrial release after photothrombotic ring stroke in rats. |
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| Authors: | Hu, Xiao‐Lei (AUTHOR), Olsson, Tommy (AUTHOR), Johansson, Inga‐Maj (AUTHOR), Brännström, Thomas (AUTHOR), Wester, Per (AUTHOR) |
| Source: | European Journal of Neuroscience. Sep2004, Vol. 20 Issue 5, p1177-1188. 12p. |
| Subjects: | Proteins, Apoptosis, Mitochondria, Cerebral ischemia, Ischemia, Mice |
| Abstract: | The anti-apoptotic proteins Bcl-w and Bcl-2 and the pro-apoptotic protein Bax may mediate cell death or survival via regulation of the mitochondria including second mitochondria-derived activator of caspase (Smac)/direct inhibitor of apoptosis protein (IAP)-binding protein with low pl (DIABLO) release. This study aimed to explore alterations in Bcl-w, Bcl-2, and Bax and the relationship between these proteins and Smac/DIABLO by means of in situ hybridization, immunohistochemical (IHC) staining, and Western blots after low- and high-intensity photothrombotic ring stroke. At 4 h after low-intensity irradiation, we found widespread bcl-w overexpression on both the mRNA and protein levels in the bilateral cortex except the ring lesion region and in subcortical regions. A prolonged elevation of Bcl-2 with relatively unchanged Bax in the mitochondrial fraction was demonstrated from 4 to 72 h. These upregulated anti-apoptotic proteins combined with little Smac/DIABLO release might be associated with increased cell survival and thereby remarkable morphological recovery after low-intensity irradiation. After high-intensity irradiation, we observed decreased bcl-w and bcl-2 mRNA with increased Bcl-2 protein in the cytosolic fraction, whereas the Bax protein remained in scattered ischaemic cells in the ring lesion and the region at risk that corresponded with release of Smac/DIABLO from mitochondria to the cytosol at 1-24 h. These changes might be related to the massive cell death observed after high-intensity irradiation. Taken together, the balance and the location of anti-apoptotic proteins vs. pro-apoptotic proteins could be associated with the translocation of Smac/DIABLO from the mitochondria to the cytosol and therefore closely related to cell death or survival after focal cerebral ischaemia. [ABSTRACT FROM AUTHOR] |
| Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
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| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 14281506 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Dynamic changes of the anti- and pro-apoptotic proteins Bcl-w, Bcl-2, and Bax with Smac/Diablo mitochondrial release after photothrombotic ring stroke in rats. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Hu%2C+Xiao‐Lei%22">Hu, Xiao‐Lei</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Olsson%2C+Tommy%22">Olsson, Tommy</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Johansson%2C+Inga‐Maj%22">Johansson, Inga‐Maj</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Brännström%2C+Thomas%22">Brännström, Thomas</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wester%2C+Per%22">Wester, Per</searchLink> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22European+Journal+of+Neuroscience%22">European Journal of Neuroscience</searchLink>. Sep2004, Vol. 20 Issue 5, p1177-1188. 12p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Proteins%22">Proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Apoptosis%22">Apoptosis</searchLink><br /><searchLink fieldCode="DE" term="%22Mitochondria%22">Mitochondria</searchLink><br /><searchLink fieldCode="DE" term="%22Cerebral+ischemia%22">Cerebral ischemia</searchLink><br /><searchLink fieldCode="DE" term="%22Ischemia%22">Ischemia</searchLink><br /><searchLink fieldCode="DE" term="%22Mice%22">Mice</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The anti-apoptotic proteins Bcl-w and Bcl-2 and the pro-apoptotic protein Bax may mediate cell death or survival via regulation of the mitochondria including second mitochondria-derived activator of caspase (Smac)/direct inhibitor of apoptosis protein (IAP)-binding protein with low pl (DIABLO) release. This study aimed to explore alterations in Bcl-w, Bcl-2, and Bax and the relationship between these proteins and Smac/DIABLO by means of in situ hybridization, immunohistochemical (IHC) staining, and Western blots after low- and high-intensity photothrombotic ring stroke. At 4 h after low-intensity irradiation, we found widespread bcl-w overexpression on both the mRNA and protein levels in the bilateral cortex except the ring lesion region and in subcortical regions. A prolonged elevation of Bcl-2 with relatively unchanged Bax in the mitochondrial fraction was demonstrated from 4 to 72 h. These upregulated anti-apoptotic proteins combined with little Smac/DIABLO release might be associated with increased cell survival and thereby remarkable morphological recovery after low-intensity irradiation. After high-intensity irradiation, we observed decreased bcl-w and bcl-2 mRNA with increased Bcl-2 protein in the cytosolic fraction, whereas the Bax protein remained in scattered ischaemic cells in the ring lesion and the region at risk that corresponded with release of Smac/DIABLO from mitochondria to the cytosol at 1-24 h. These changes might be related to the massive cell death observed after high-intensity irradiation. Taken together, the balance and the location of anti-apoptotic proteins vs. pro-apoptotic proteins could be associated with the translocation of Smac/DIABLO from the mitochondria to the cytosol and therefore closely related to cell death or survival after focal cerebral ischaemia. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1111/j.1460-9568.2004.03554.x Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 12 StartPage: 1177 Subjects: – SubjectFull: Proteins Type: general – SubjectFull: Apoptosis Type: general – SubjectFull: Mitochondria Type: general – SubjectFull: Cerebral ischemia Type: general – SubjectFull: Ischemia Type: general – SubjectFull: Mice Type: general Titles: – TitleFull: Dynamic changes of the anti- and pro-apoptotic proteins Bcl-w, Bcl-2, and Bax with Smac/Diablo mitochondrial release after photothrombotic ring stroke in rats. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Hu, Xiao‐Lei – PersonEntity: Name: NameFull: Olsson, Tommy – PersonEntity: Name: NameFull: Johansson, Inga‐Maj – PersonEntity: Name: NameFull: Brännström, Thomas – PersonEntity: Name: NameFull: Wester, Per IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 09 Text: Sep2004 Type: published Y: 2004 Identifiers: – Type: issn-print Value: 0953816X Numbering: – Type: volume Value: 20 – Type: issue Value: 5 Titles: – TitleFull: European Journal of Neuroscience Type: main |
| ResultId | 1 |
