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Potent neutralizing antibodies from COVID-19 patients define multiple targets of vulnerability.

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Title: Potent neutralizing antibodies from COVID-19 patients define multiple targets of vulnerability.
Authors: Brouwer, Philip J. M., Caniels, Tom G., van der Straten, Karlijn, Snitselaar, Jonne L., Aldon, Yoann, Bangaru, Sandhya, Torres, Jonathan L., Okba, Nisreen M. A., Claireaux, Mathieu, Kerster, Gius, Bentlage, Arthur E. H., van Haaren, Marlies M., Guerra, Denise, Burger, Judith A., Schermer, Edith E., Verheu, Kirsten D., van der Velde, Niels, van der Kooi, Alex, van Schooten, Jelle, van Breemen, Mariëlle J.
Source: Science (pre-March 2025). 8/7/2020, Vol. 369 Issue 6504, p643-650. 7p. 4 Color Photographs.
Subjects: SARS disease, COVID-19 pandemic, Monoclonal antibodies, COVID-19 testing, Epitopes, COVID-19 vaccines
Abstract: The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has had a large impact on global health, travel, and economy. Therefore, preventative and therapeutic measures are urgently needed. Here, we isolated monoclonal antibodies from three convalescent coronavirus disease 2019 (COVID-19) patients using a SARS-CoV-2 stabilized prefusion spike protein. These antibodies had low levels of somatic hypermutation and showed a strong enrichment in VH1-69, VH3-30-3, and VH1-24 gene usage. A subset of the antibodies was able to potently inhibit authentic SARS-CoV-2 infection at a concentration as low as 0.007 micrograms per milliliter. Competition and electron microscopy studies illustrate that the SARS-CoV-2 spike protein contains multiple distinct antigenic sites, including several receptor-binding domain (RBD) epitopes as well as non-RBD epitopes. In addition to providing guidance for vaccine design, the antibodies described here are promising candidates for COVID-19 treatment and prevention. [ABSTRACT FROM AUTHOR]
Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Data: Potent neutralizing antibodies from COVID-19 patients define multiple targets of vulnerability.
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  Data: <searchLink fieldCode="AR" term="%22Brouwer%2C+Philip+J%2E+M%2E%22">Brouwer, Philip J. M.</searchLink><br /><searchLink fieldCode="AR" term="%22Caniels%2C+Tom+G%2E%22">Caniels, Tom G.</searchLink><br /><searchLink fieldCode="AR" term="%22van+der+Straten%2C+Karlijn%22">van der Straten, Karlijn</searchLink><br /><searchLink fieldCode="AR" term="%22Snitselaar%2C+Jonne+L%2E%22">Snitselaar, Jonne L.</searchLink><br /><searchLink fieldCode="AR" term="%22Aldon%2C+Yoann%22">Aldon, Yoann</searchLink><br /><searchLink fieldCode="AR" term="%22Bangaru%2C+Sandhya%22">Bangaru, Sandhya</searchLink><br /><searchLink fieldCode="AR" term="%22Torres%2C+Jonathan+L%2E%22">Torres, Jonathan L.</searchLink><br /><searchLink fieldCode="AR" term="%22Okba%2C+Nisreen+M%2E+A%2E%22">Okba, Nisreen M. A.</searchLink><br /><searchLink fieldCode="AR" term="%22Claireaux%2C+Mathieu%22">Claireaux, Mathieu</searchLink><br /><searchLink fieldCode="AR" term="%22Kerster%2C+Gius%22">Kerster, Gius</searchLink><br /><searchLink fieldCode="AR" term="%22Bentlage%2C+Arthur+E%2E+H%2E%22">Bentlage, Arthur E. H.</searchLink><br /><searchLink fieldCode="AR" term="%22van+Haaren%2C+Marlies+M%2E%22">van Haaren, Marlies M.</searchLink><br /><searchLink fieldCode="AR" term="%22Guerra%2C+Denise%22">Guerra, Denise</searchLink><br /><searchLink fieldCode="AR" term="%22Burger%2C+Judith+A%2E%22">Burger, Judith A.</searchLink><br /><searchLink fieldCode="AR" term="%22Schermer%2C+Edith+E%2E%22">Schermer, Edith E.</searchLink><br /><searchLink fieldCode="AR" term="%22Verheu%2C+Kirsten+D%2E%22">Verheu, Kirsten D.</searchLink><br /><searchLink fieldCode="AR" term="%22van+der+Velde%2C+Niels%22">van der Velde, Niels</searchLink><br /><searchLink fieldCode="AR" term="%22van+der+Kooi%2C+Alex%22">van der Kooi, Alex</searchLink><br /><searchLink fieldCode="AR" term="%22van+Schooten%2C+Jelle%22">van Schooten, Jelle</searchLink><br /><searchLink fieldCode="AR" term="%22van+Breemen%2C+Mariëlle+J%2E%22">van Breemen, Mariëlle J.</searchLink>
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  Data: <searchLink fieldCode="JN" term="%22Science+%28pre-March+2025%29%22">Science (pre-March 2025)</searchLink>. 8/7/2020, Vol. 369 Issue 6504, p643-650. 7p. 4 Color Photographs.
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  Data: <searchLink fieldCode="DE" term="%22SARS+disease%22">SARS disease</searchLink><br /><searchLink fieldCode="DE" term="%22COVID-19+pandemic%22">COVID-19 pandemic</searchLink><br /><searchLink fieldCode="DE" term="%22Monoclonal+antibodies%22">Monoclonal antibodies</searchLink><br /><searchLink fieldCode="DE" term="%22COVID-19+testing%22">COVID-19 testing</searchLink><br /><searchLink fieldCode="DE" term="%22Epitopes%22">Epitopes</searchLink><br /><searchLink fieldCode="DE" term="%22COVID-19+vaccines%22">COVID-19 vaccines</searchLink>
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  Label: Abstract
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  Data: The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has had a large impact on global health, travel, and economy. Therefore, preventative and therapeutic measures are urgently needed. Here, we isolated monoclonal antibodies from three convalescent coronavirus disease 2019 (COVID-19) patients using a SARS-CoV-2 stabilized prefusion spike protein. These antibodies had low levels of somatic hypermutation and showed a strong enrichment in VH1-69, VH3-30-3, and VH1-24 gene usage. A subset of the antibodies was able to potently inhibit authentic SARS-CoV-2 infection at a concentration as low as 0.007 micrograms per milliliter. Competition and electron microscopy studies illustrate that the SARS-CoV-2 spike protein contains multiple distinct antigenic sites, including several receptor-binding domain (RBD) epitopes as well as non-RBD epitopes. In addition to providing guidance for vaccine design, the antibodies described here are promising candidates for COVID-19 treatment and prevention. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=145015113
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