Effects of GABAA receptors in nucleus cuneiformis on the cannabinoid antinociception using the formalin test.
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| Title: | Effects of GABAA receptors in nucleus cuneiformis on the cannabinoid antinociception using the formalin test. |
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| Authors: | Chen, Junjie (AUTHOR), Hasanein, Parisa (AUTHOR), Komaki, Alireza (AUTHOR), Yari, Siamak (AUTHOR) |
| Source: | Psychopharmacology. Jun2021, Vol. 238 Issue 6, p1657-1669. 13p. 1 Diagram, 4 Graphs. |
| Subjects: | Formaldehyde, Cannabinoid receptors, Pain management, Pain perception, Microinjections, GABA |
| Abstract: | Rationale: Nucleus cuneiformis (NC), a reticular nucleus of the midbrain, is a part of the descending pain modulatory system and therefore has an important role in pain perception. Objectives: Considering the abundance of GABAA and cannabinoid receptors in the NC and also the bidirectional roles for GABA in controlling nociception, the present study examined the effects of bilateral intra-NC microinjection of different doses of the GABAA receptor agonist, muscimol, and the GABAA receptor antagonist, bicuculline, on pain modulation using formalin test. We also assessed interaction between canabinergic and GABAergic systems in the NC during this test. Methods: Rats were exposed to intra-NC microinjection of bicuculline (50,100, and 200 ng/side) or muscimol (60, 120, and 240 ng/side) and then subjected to the formalin test. In another set of experiments, the effects of muscimol (60 ng/side) or bicuculline (50 ng/side) administration 5 min before a cannabinoid receptor agonist WIN 55,212-2 (5, 10, and 20 μg/side) microinjection into NC on the formalin test were evaluated. Results: Microinjection of bicuculline and muscimol into the NC decreased and increased pain responses, respectively, in a dose-dependent manner during both phases of the test. Microinjection of WIN 55,212-2 into the NC significantly reduced pain responses in a dose-dependent manner. Microinjection of bicuculline or muscimol in combination with WIN 55,212-2 into the NC respectively potentiated and attenuated WIN 55,212-2–induced antinociception in the formalin test. Conclusions: This study shows that GABA in the NC is involved in pain modulation and suggests the existence of a GABAA-mediated inhibitory system in the NC on pain control. Furthermore, it seems that the antinociceptive effect of WIN 55,212-2 in the formalin test is mediated partly by the activity of local GABAA receptors in the NC. [ABSTRACT FROM AUTHOR] |
| Copyright of Psychopharmacology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 150429684 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Effects of GABAA receptors in nucleus cuneiformis on the cannabinoid antinociception using the formalin test. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Chen%2C+Junjie%22">Chen, Junjie</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hasanein%2C+Parisa%22">Hasanein, Parisa</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Komaki%2C+Alireza%22">Komaki, Alireza</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yari%2C+Siamak%22">Yari, Siamak</searchLink> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Psychopharmacology%22">Psychopharmacology</searchLink>. Jun2021, Vol. 238 Issue 6, p1657-1669. 13p. 1 Diagram, 4 Graphs. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Formaldehyde%22">Formaldehyde</searchLink><br /><searchLink fieldCode="DE" term="%22Cannabinoid+receptors%22">Cannabinoid receptors</searchLink><br /><searchLink fieldCode="DE" term="%22Pain+management%22">Pain management</searchLink><br /><searchLink fieldCode="DE" term="%22Pain+perception%22">Pain perception</searchLink><br /><searchLink fieldCode="DE" term="%22Microinjections%22">Microinjections</searchLink><br /><searchLink fieldCode="DE" term="%22GABA%22">GABA</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Rationale: Nucleus cuneiformis (NC), a reticular nucleus of the midbrain, is a part of the descending pain modulatory system and therefore has an important role in pain perception. Objectives: Considering the abundance of GABAA and cannabinoid receptors in the NC and also the bidirectional roles for GABA in controlling nociception, the present study examined the effects of bilateral intra-NC microinjection of different doses of the GABAA receptor agonist, muscimol, and the GABAA receptor antagonist, bicuculline, on pain modulation using formalin test. We also assessed interaction between canabinergic and GABAergic systems in the NC during this test. Methods: Rats were exposed to intra-NC microinjection of bicuculline (50,100, and 200 ng/side) or muscimol (60, 120, and 240 ng/side) and then subjected to the formalin test. In another set of experiments, the effects of muscimol (60 ng/side) or bicuculline (50 ng/side) administration 5 min before a cannabinoid receptor agonist WIN 55,212-2 (5, 10, and 20 μg/side) microinjection into NC on the formalin test were evaluated. Results: Microinjection of bicuculline and muscimol into the NC decreased and increased pain responses, respectively, in a dose-dependent manner during both phases of the test. Microinjection of WIN 55,212-2 into the NC significantly reduced pain responses in a dose-dependent manner. Microinjection of bicuculline or muscimol in combination with WIN 55,212-2 into the NC respectively potentiated and attenuated WIN 55,212-2–induced antinociception in the formalin test. Conclusions: This study shows that GABA in the NC is involved in pain modulation and suggests the existence of a GABAA-mediated inhibitory system in the NC on pain control. Furthermore, it seems that the antinociceptive effect of WIN 55,212-2 in the formalin test is mediated partly by the activity of local GABAA receptors in the NC. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Psychopharmacology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00213-021-05800-3 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 13 StartPage: 1657 Subjects: – SubjectFull: Formaldehyde Type: general – SubjectFull: Cannabinoid receptors Type: general – SubjectFull: Pain management Type: general – SubjectFull: Pain perception Type: general – SubjectFull: Microinjections Type: general – SubjectFull: GABA Type: general Titles: – TitleFull: Effects of GABAA receptors in nucleus cuneiformis on the cannabinoid antinociception using the formalin test. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Chen, Junjie – PersonEntity: Name: NameFull: Hasanein, Parisa – PersonEntity: Name: NameFull: Komaki, Alireza – PersonEntity: Name: NameFull: Yari, Siamak IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 06 Text: Jun2021 Type: published Y: 2021 Identifiers: – Type: issn-print Value: 00333158 Numbering: – Type: volume Value: 238 – Type: issue Value: 6 Titles: – TitleFull: Psychopharmacology Type: main |
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