View in EDS HTML Full Text PDF Full Text

Evolution of a virus-like architecture and packaging mechanism in a repurposed bacterial protein.

Saved in:
Bibliographic Details
Title: Evolution of a virus-like architecture and packaging mechanism in a repurposed bacterial protein.
Authors: Tetter, Stephan, Terasaka, Naohiro, Steinauer, Angela, Bingham, Richard J., Clark, Sam, Scott, Andrew J. P., Patel, Nikesh, Leibundgut, Marc, Wroblewski, Emma, Ban, Nenad, Stockley, Peter G., Twarock, Reidun, Hilvert, Donald
Source: Science (pre-March 2025). 6/11/2021, Vol. 372 Issue 6547, p1220-1224. 5p. 2 Color Photographs, 2 Diagrams.
Subjects: Virion, Bacterial protein structure, Viral evolution, Bacterial enzymes synthesis, Nucleocapsids, Messenger RNA
Abstract: Viruses are ubiquitous pathogens of global impact. Prompted by the hypothesis that their earliest progenitors recruited host proteins for virion formation, we have used stringent laboratory evolution to convert a bacterial enzyme that lacks affinity for nucleic acids into an artificial nucleocapsid that efficiently packages and protects multiple copies of its own encoding messenger RNA. Revealing remarkable convergence on the molecular hallmarks of natural viruses, the accompanying changes reorganized the protein building blocks into an interlaced 240-subunit icosahedral capsid that is impermeable to nucleases, and emergence of a robust RNA stem-loop packaging cassette ensured high encapsidation yields and specificity. In addition to evincing a plausible evolutionary pathway for primordial viruses, these findings highlight practical strategies for developing nonviral carriers for diverse vaccine and delivery applications. [ABSTRACT FROM AUTHOR]
Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
Full text is not displayed to guests.
FullText Links:
  – Type: pdflink
Text:
  Availability: 1
Header DbId: pbh
DbLabel: Psychology and Behavioral Sciences Collection
An: 151014909
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Evolution of a virus-like architecture and packaging mechanism in a repurposed bacterial protein.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Tetter%2C+Stephan%22">Tetter, Stephan</searchLink><br /><searchLink fieldCode="AR" term="%22Terasaka%2C+Naohiro%22">Terasaka, Naohiro</searchLink><br /><searchLink fieldCode="AR" term="%22Steinauer%2C+Angela%22">Steinauer, Angela</searchLink><br /><searchLink fieldCode="AR" term="%22Bingham%2C+Richard+J%2E%22">Bingham, Richard J.</searchLink><br /><searchLink fieldCode="AR" term="%22Clark%2C+Sam%22">Clark, Sam</searchLink><br /><searchLink fieldCode="AR" term="%22Scott%2C+Andrew+J%2E+P%2E%22">Scott, Andrew J. P.</searchLink><br /><searchLink fieldCode="AR" term="%22Patel%2C+Nikesh%22">Patel, Nikesh</searchLink><br /><searchLink fieldCode="AR" term="%22Leibundgut%2C+Marc%22">Leibundgut, Marc</searchLink><br /><searchLink fieldCode="AR" term="%22Wroblewski%2C+Emma%22">Wroblewski, Emma</searchLink><br /><searchLink fieldCode="AR" term="%22Ban%2C+Nenad%22">Ban, Nenad</searchLink><br /><searchLink fieldCode="AR" term="%22Stockley%2C+Peter+G%2E%22">Stockley, Peter G.</searchLink><br /><searchLink fieldCode="AR" term="%22Twarock%2C+Reidun%22">Twarock, Reidun</searchLink><br /><searchLink fieldCode="AR" term="%22Hilvert%2C+Donald%22">Hilvert, Donald</searchLink>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Science+%28pre-March+2025%29%22">Science (pre-March 2025)</searchLink>. 6/11/2021, Vol. 372 Issue 6547, p1220-1224. 5p. 2 Color Photographs, 2 Diagrams.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Virion%22">Virion</searchLink><br /><searchLink fieldCode="DE" term="%22Bacterial+protein+structure%22">Bacterial protein structure</searchLink><br /><searchLink fieldCode="DE" term="%22Viral+evolution%22">Viral evolution</searchLink><br /><searchLink fieldCode="DE" term="%22Bacterial+enzymes+synthesis%22">Bacterial enzymes synthesis</searchLink><br /><searchLink fieldCode="DE" term="%22Nucleocapsids%22">Nucleocapsids</searchLink><br /><searchLink fieldCode="DE" term="%22Messenger+RNA%22">Messenger RNA</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Viruses are ubiquitous pathogens of global impact. Prompted by the hypothesis that their earliest progenitors recruited host proteins for virion formation, we have used stringent laboratory evolution to convert a bacterial enzyme that lacks affinity for nucleic acids into an artificial nucleocapsid that efficiently packages and protects multiple copies of its own encoding messenger RNA. Revealing remarkable convergence on the molecular hallmarks of natural viruses, the accompanying changes reorganized the protein building blocks into an interlaced 240-subunit icosahedral capsid that is impermeable to nucleases, and emergence of a robust RNA stem-loop packaging cassette ensured high encapsidation yields and specificity. In addition to evincing a plausible evolutionary pathway for primordial viruses, these findings highlight practical strategies for developing nonviral carriers for diverse vaccine and delivery applications. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=151014909
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1126/science.abg2822
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 5
        StartPage: 1220
    Subjects:
      – SubjectFull: Virion
        Type: general
      – SubjectFull: Bacterial protein structure
        Type: general
      – SubjectFull: Viral evolution
        Type: general
      – SubjectFull: Bacterial enzymes synthesis
        Type: general
      – SubjectFull: Nucleocapsids
        Type: general
      – SubjectFull: Messenger RNA
        Type: general
    Titles:
      – TitleFull: Evolution of a virus-like architecture and packaging mechanism in a repurposed bacterial protein.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Tetter, Stephan
      – PersonEntity:
          Name:
            NameFull: Terasaka, Naohiro
      – PersonEntity:
          Name:
            NameFull: Steinauer, Angela
      – PersonEntity:
          Name:
            NameFull: Bingham, Richard J.
      – PersonEntity:
          Name:
            NameFull: Clark, Sam
      – PersonEntity:
          Name:
            NameFull: Scott, Andrew J. P.
      – PersonEntity:
          Name:
            NameFull: Patel, Nikesh
      – PersonEntity:
          Name:
            NameFull: Leibundgut, Marc
      – PersonEntity:
          Name:
            NameFull: Wroblewski, Emma
      – PersonEntity:
          Name:
            NameFull: Ban, Nenad
      – PersonEntity:
          Name:
            NameFull: Stockley, Peter G.
      – PersonEntity:
          Name:
            NameFull: Twarock, Reidun
      – PersonEntity:
          Name:
            NameFull: Hilvert, Donald
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 11
              M: 06
              Text: 6/11/2021
              Type: published
              Y: 2021
          Identifiers:
            – Type: issn-print
              Value: 00368075
          Numbering:
            – Type: volume
              Value: 372
            – Type: issue
              Value: 6547
          Titles:
            – TitleFull: Science (pre-March 2025)
              Type: main
ResultId 1