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MiR‐205 is up‐regulated in islets of diabetes‐susceptible mice and targets the diabetes gene Tcf7l2.

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Title: MiR‐205 is up‐regulated in islets of diabetes‐susceptible mice and targets the diabetes gene Tcf7l2.
Authors: Ouni, Meriem (AUTHOR), Gottmann, Pascal (AUTHOR), Westholm, Efraim (AUTHOR), Schwerbel, Kristin (AUTHOR), Jähnert, Markus (AUTHOR), Stadion, Mandy (AUTHOR), Rittig, Kilian (AUTHOR), Vogel, Heike (AUTHOR), Schürmann, Annette (AUTHOR)
Source: Acta Physiologica. Aug2021, Vol. 232 Issue 4, p1-20. 20p. 2 Diagrams, 4 Charts, 5 Graphs.
Subjects: Islands, MicroRNA, Gene targeting, Wnt genes, Wnt signal transduction, Insulin, Genetic regulation
Abstract: Aim: MicroRNAs play an important role in the maintenance of cellular functions by fine‐tuning gene expression levels. The aim of the current study was to identify genetically caused changes in microRNA expression which associate with islet dysfunction in diabetic mice. Methods: To identify novel microRNAs involved in islet dysfunction, transcriptome and miRNome analyses were performed in islets of obese, diabetes‐susceptible NZO and diabetes‐resistant B6‐ob/ob mice and results combined with quantitative trait loci (QTL) and functional in vitro analysis. Results: In islets of NZO and B6‐ob/ob mice, 94 differentially expressed microRNAs were detected, of which 11 are located in diabetes QTL. Focusing on conserved microRNAs exhibiting the strongest expression difference and which have not been linked to islet function, miR‐205‐5p was selected for further analysis. According to transcriptome data and target prediction analyses, miR‐205‐5p affects genes involved in Wnt and calcium signalling as well as insulin secretion. Over‐expression of miR‐205‐5p in the insulinoma cell line INS‐1 increased insulin expression, left‐shifted the glucose‐dependence of insulin secretion and supressed the expression of the diabetes gene TCF7L2. The interaction between miR‐205‐5p and TCF7L2 was confirmed by luciferase reporter assay. Conclusion: MiR‐205‐5p was identified as relevant microRNA involved in islet dysfunction by interacting with TCF7L2. [ABSTRACT FROM AUTHOR]
Copyright of Acta Physiologica is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Data: MiR‐205 is up‐regulated in islets of diabetes‐susceptible mice and targets the diabetes gene Tcf7l2.
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  Data: <searchLink fieldCode="AR" term="%22Ouni%2C+Meriem%22">Ouni, Meriem</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Gottmann%2C+Pascal%22">Gottmann, Pascal</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Westholm%2C+Efraim%22">Westholm, Efraim</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Schwerbel%2C+Kristin%22">Schwerbel, Kristin</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Jähnert%2C+Markus%22">Jähnert, Markus</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Stadion%2C+Mandy%22">Stadion, Mandy</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Rittig%2C+Kilian%22">Rittig, Kilian</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Vogel%2C+Heike%22">Vogel, Heike</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Schürmann%2C+Annette%22">Schürmann, Annette</searchLink> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22Acta+Physiologica%22">Acta Physiologica</searchLink>. Aug2021, Vol. 232 Issue 4, p1-20. 20p. 2 Diagrams, 4 Charts, 5 Graphs.
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  Data: <searchLink fieldCode="DE" term="%22Islands%22">Islands</searchLink><br /><searchLink fieldCode="DE" term="%22MicroRNA%22">MicroRNA</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+targeting%22">Gene targeting</searchLink><br /><searchLink fieldCode="DE" term="%22Wnt+genes%22">Wnt genes</searchLink><br /><searchLink fieldCode="DE" term="%22Wnt+signal+transduction%22">Wnt signal transduction</searchLink><br /><searchLink fieldCode="DE" term="%22Insulin%22">Insulin</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+regulation%22">Genetic regulation</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Aim: MicroRNAs play an important role in the maintenance of cellular functions by fine‐tuning gene expression levels. The aim of the current study was to identify genetically caused changes in microRNA expression which associate with islet dysfunction in diabetic mice. Methods: To identify novel microRNAs involved in islet dysfunction, transcriptome and miRNome analyses were performed in islets of obese, diabetes‐susceptible NZO and diabetes‐resistant B6‐ob/ob mice and results combined with quantitative trait loci (QTL) and functional in vitro analysis. Results: In islets of NZO and B6‐ob/ob mice, 94 differentially expressed microRNAs were detected, of which 11 are located in diabetes QTL. Focusing on conserved microRNAs exhibiting the strongest expression difference and which have not been linked to islet function, miR‐205‐5p was selected for further analysis. According to transcriptome data and target prediction analyses, miR‐205‐5p affects genes involved in Wnt and calcium signalling as well as insulin secretion. Over‐expression of miR‐205‐5p in the insulinoma cell line INS‐1 increased insulin expression, left‐shifted the glucose‐dependence of insulin secretion and supressed the expression of the diabetes gene TCF7L2. The interaction between miR‐205‐5p and TCF7L2 was confirmed by luciferase reporter assay. Conclusion: MiR‐205‐5p was identified as relevant microRNA involved in islet dysfunction by interacting with TCF7L2. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Acta Physiologica is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1111/apha.13693
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      – SubjectFull: Genetic regulation
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      – TitleFull: MiR‐205 is up‐regulated in islets of diabetes‐susceptible mice and targets the diabetes gene Tcf7l2.
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              Text: Aug2021
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              Y: 2021
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