Evidence for PKD2L1‐positive neurons distant from the central canal in the ventromedial spinal cord and medulla of the adult mouse.
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| Title: | Evidence for PKD2L1‐positive neurons distant from the central canal in the ventromedial spinal cord and medulla of the adult mouse. |
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| Authors: | Jurčić, Nina (AUTHOR), Michelle, Caroline (AUTHOR), Trouslard, Jérôme (AUTHOR), Wanaverbecq, Nicolas (AUTHOR), Kastner, Anne (AUTHOR) |
| Source: | European Journal of Neuroscience. Aug2021, Vol. 54 Issue 3, p4781-4803. 23p. 7 Color Photographs, 2 Graphs. |
| Subjects: | Spinal canal, Spinal cord, TRP channels, Dendrites, Stem cell niches, Cerebrospinal fluid, Adults |
| Abstract: | Neurons in contact with the cerebrospinal fluid (CSF) are found around the medullo‐spinal central canal (CC) in adult mice. These neurons (CSF‐cNs), located within or below the ependymal cell layer, known as the stem cell niche, present a characteristic morphology with a dendrite projecting to the CC and ending with a protrusion. They are GABAergic, present an intermediate neuronal maturity and selectively express PKD2L1, a member of the transient receptor potential channel superfamily with sensory properties. Using immunohistological and electrophysiological recording techniques in mice, we characterize the properties of a new population of PKD2L1 positive cells that is distant from the CC in a zone enriched with astrocytes and ependymal fibers of the ventro‐medial spinal cord and medulla. They appear around embryonic day 16 and their number increases up to early postnatal days. With development and the reorganization of the CC region, they progressively become more distant from the CC, suggesting some migratory capabilities. These neurons share functional and phenotypical properties with CSF‐cNs but appear subdivided in two groups. One group, present along the midline, has a bipolar morphology and extends a long dendrite along ependymal fibers and towards the CC. The second group, localized in more ventro‐lateral regions, has a multipolar morphology and no apparent projection to the CC. Altogether, we describe a novel population of PKD2L1+ neurons distant from the CC but with properties similar to CSF‐cNs that might serve to sense modification in the composition of either CSF or interstitial liquid, a function that will need to be confirmed. [ABSTRACT FROM AUTHOR] |
| Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 151911487 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Evidence for PKD2L1‐positive neurons distant from the central canal in the ventromedial spinal cord and medulla of the adult mouse. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Jurčić%2C+Nina%22">Jurčić, Nina</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Michelle%2C+Caroline%22">Michelle, Caroline</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Trouslard%2C+Jérôme%22">Trouslard, Jérôme</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wanaverbecq%2C+Nicolas%22">Wanaverbecq, Nicolas</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kastner%2C+Anne%22">Kastner, Anne</searchLink> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22European+Journal+of+Neuroscience%22">European Journal of Neuroscience</searchLink>. Aug2021, Vol. 54 Issue 3, p4781-4803. 23p. 7 Color Photographs, 2 Graphs. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Spinal+canal%22">Spinal canal</searchLink><br /><searchLink fieldCode="DE" term="%22Spinal+cord%22">Spinal cord</searchLink><br /><searchLink fieldCode="DE" term="%22TRP+channels%22">TRP channels</searchLink><br /><searchLink fieldCode="DE" term="%22Dendrites%22">Dendrites</searchLink><br /><searchLink fieldCode="DE" term="%22Stem+cell+niches%22">Stem cell niches</searchLink><br /><searchLink fieldCode="DE" term="%22Cerebrospinal+fluid%22">Cerebrospinal fluid</searchLink><br /><searchLink fieldCode="DE" term="%22Adults%22">Adults</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Neurons in contact with the cerebrospinal fluid (CSF) are found around the medullo‐spinal central canal (CC) in adult mice. These neurons (CSF‐cNs), located within or below the ependymal cell layer, known as the stem cell niche, present a characteristic morphology with a dendrite projecting to the CC and ending with a protrusion. They are GABAergic, present an intermediate neuronal maturity and selectively express PKD2L1, a member of the transient receptor potential channel superfamily with sensory properties. Using immunohistological and electrophysiological recording techniques in mice, we characterize the properties of a new population of PKD2L1 positive cells that is distant from the CC in a zone enriched with astrocytes and ependymal fibers of the ventro‐medial spinal cord and medulla. They appear around embryonic day 16 and their number increases up to early postnatal days. With development and the reorganization of the CC region, they progressively become more distant from the CC, suggesting some migratory capabilities. These neurons share functional and phenotypical properties with CSF‐cNs but appear subdivided in two groups. One group, present along the midline, has a bipolar morphology and extends a long dendrite along ependymal fibers and towards the CC. The second group, localized in more ventro‐lateral regions, has a multipolar morphology and no apparent projection to the CC. Altogether, we describe a novel population of PKD2L1+ neurons distant from the CC but with properties similar to CSF‐cNs that might serve to sense modification in the composition of either CSF or interstitial liquid, a function that will need to be confirmed. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1111/ejn.15342 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 23 StartPage: 4781 Subjects: – SubjectFull: Spinal canal Type: general – SubjectFull: Spinal cord Type: general – SubjectFull: TRP channels Type: general – SubjectFull: Dendrites Type: general – SubjectFull: Stem cell niches Type: general – SubjectFull: Cerebrospinal fluid Type: general – SubjectFull: Adults Type: general Titles: – TitleFull: Evidence for PKD2L1‐positive neurons distant from the central canal in the ventromedial spinal cord and medulla of the adult mouse. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Jurčić, Nina – PersonEntity: Name: NameFull: Michelle, Caroline – PersonEntity: Name: NameFull: Trouslard, Jérôme – PersonEntity: Name: NameFull: Wanaverbecq, Nicolas – PersonEntity: Name: NameFull: Kastner, Anne IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 08 Text: Aug2021 Type: published Y: 2021 Identifiers: – Type: issn-print Value: 0953816X Numbering: – Type: volume Value: 54 – Type: issue Value: 3 Titles: – TitleFull: European Journal of Neuroscience Type: main |
| ResultId | 1 |
