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microRNA expression levels in the nucleus accumbens correlate with morphine‐taking but not morphine‐seeking behaviour in male rats.

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Title: microRNA expression levels in the nucleus accumbens correlate with morphine‐taking but not morphine‐seeking behaviour in male rats.
Authors: Gillespie, Aria (AUTHOR), Mayberry, Hannah L. (AUTHOR), Wimmer, Mathieu E. (AUTHOR), Sillivan, Stephanie E. (AUTHOR)
Source: European Journal of Neuroscience. Apr2022, Vol. 55 Issue 7, p1742-1755. 14p. 2 Charts, 3 Graphs.
Subjects: Nucleus accumbens, MicroRNA, Non-coding RNA, RNA regulation, Drug side effects
Abstract: A powerful motivation to seek opioids remains after drug cessation and intensifies during extended periods of abstinence. Unfortunately, biomarkers associated with continued drug seeking have not been described. Moreover, previous studies have focused on the effects of early abstinence with little exploration into the long‐term drug‐induced mechanisms that occur after extended abstinence. Here we demonstrated that 30 days (D) of forced abstinence results in a time‐dependent increase in morphine seeking in a rat model of morphine self‐administration (SA). We measured expression of known drug‐responsive microRNAs (miRNAs) in the nucleus accumbens, an area critical for reward‐related plasticity, during early or late abstinence in animals that underwent either a cue‐induced relapse test or no relapse test. miRNAs are small noncoding RNAs that represent suitable biomarker candidates due to their long‐lasting nature. mir‐32‐5p levels during early abstinence negatively correlated with active lever pressing in both cue‐exposed and cue‐naïve animals. mir‐1298‐5p positively correlated with drug SA history after a relapse test during late abstinence. When animals underwent acute abstinence with no relapse test, mir‐1298‐5p correlated with drug infusions and active lever pressing during SA. In late abstinence with no relapse test, mir‐137‐3p negatively correlated with drug infusions. Regulation of mir‐32‐5p target genes and significant correlation of target gene mRNA with mir‐32‐5p was observed after abstinence. These results indicate that lasting regulation of miRNA expression is associated with drug intake following morphine SA. In addition, we conclude that the miRNA profile undergoes regulation from early to late abstinence and miRNA expression may indicate past drug history. [ABSTRACT FROM AUTHOR]
Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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Items – Name: Title
  Label: Title
  Group: Ti
  Data: microRNA expression levels in the nucleus accumbens correlate with morphine‐taking but not morphine‐seeking behaviour in male rats.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Gillespie%2C+Aria%22">Gillespie, Aria</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mayberry%2C+Hannah+L%2E%22">Mayberry, Hannah L.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wimmer%2C+Mathieu+E%2E%22">Wimmer, Mathieu E.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sillivan%2C+Stephanie+E%2E%22">Sillivan, Stephanie E.</searchLink> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22European+Journal+of+Neuroscience%22">European Journal of Neuroscience</searchLink>. Apr2022, Vol. 55 Issue 7, p1742-1755. 14p. 2 Charts, 3 Graphs.
– Name: Subject
  Label: Subjects
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  Data: <searchLink fieldCode="DE" term="%22Nucleus+accumbens%22">Nucleus accumbens</searchLink><br /><searchLink fieldCode="DE" term="%22MicroRNA%22">MicroRNA</searchLink><br /><searchLink fieldCode="DE" term="%22Non-coding+RNA%22">Non-coding RNA</searchLink><br /><searchLink fieldCode="DE" term="%22RNA+regulation%22">RNA regulation</searchLink><br /><searchLink fieldCode="DE" term="%22Drug+side+effects%22">Drug side effects</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: A powerful motivation to seek opioids remains after drug cessation and intensifies during extended periods of abstinence. Unfortunately, biomarkers associated with continued drug seeking have not been described. Moreover, previous studies have focused on the effects of early abstinence with little exploration into the long‐term drug‐induced mechanisms that occur after extended abstinence. Here we demonstrated that 30 days (D) of forced abstinence results in a time‐dependent increase in morphine seeking in a rat model of morphine self‐administration (SA). We measured expression of known drug‐responsive microRNAs (miRNAs) in the nucleus accumbens, an area critical for reward‐related plasticity, during early or late abstinence in animals that underwent either a cue‐induced relapse test or no relapse test. miRNAs are small noncoding RNAs that represent suitable biomarker candidates due to their long‐lasting nature. mir‐32‐5p levels during early abstinence negatively correlated with active lever pressing in both cue‐exposed and cue‐naïve animals. mir‐1298‐5p positively correlated with drug SA history after a relapse test during late abstinence. When animals underwent acute abstinence with no relapse test, mir‐1298‐5p correlated with drug infusions and active lever pressing during SA. In late abstinence with no relapse test, mir‐137‐3p negatively correlated with drug infusions. Regulation of mir‐32‐5p target genes and significant correlation of target gene mRNA with mir‐32‐5p was observed after abstinence. These results indicate that lasting regulation of miRNA expression is associated with drug intake following morphine SA. In addition, we conclude that the miRNA profile undergoes regulation from early to late abstinence and miRNA expression may indicate past drug history. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1111/ejn.15650
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      – Code: eng
        Text: English
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        PageCount: 14
        StartPage: 1742
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      – SubjectFull: Nucleus accumbens
        Type: general
      – SubjectFull: MicroRNA
        Type: general
      – SubjectFull: Non-coding RNA
        Type: general
      – SubjectFull: RNA regulation
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      – SubjectFull: Drug side effects
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      – TitleFull: microRNA expression levels in the nucleus accumbens correlate with morphine‐taking but not morphine‐seeking behaviour in male rats.
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            NameFull: Gillespie, Aria
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            NameFull: Mayberry, Hannah L.
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            NameFull: Wimmer, Mathieu E.
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            – D: 01
              M: 04
              Text: Apr2022
              Type: published
              Y: 2022
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