Mechanism of hsp70i Gene Bookmarking.
Saved in:
| Title: | Mechanism of hsp70i Gene Bookmarking. |
|---|---|
| Authors: | Xing, Hongyan, Wilkerson, Donald C., Mayhew, Christopher N., Lubert, Eric J., Skaggs, Hollie S., Goodson, Michael L, Hong, Yiling, Park-Sarge, Ok-Kyong, Sarge, Kevin D. |
| Source: | Science (pre-March 2025). 1/21/2005, Vol. 307 Issue 5708, p421-423. 3p. |
| Subjects: | Nucleic acids, Cells, Genes, Proteins, Heat shock proteins, Transcription factors |
| Abstract: | In contrast to most genomic DNA in mitotic cells, the promoterregions of some genes, such as the stress-inducible hsp70i gene thatcodes for a heat shock protein, remain uncompacted, a phenomenon calledbookmarking. Here we show that hsp70i bookmarking is mediated by atranscription factor called HSF2, which binds this promoter in mitoticcells, recruits protein phosphatase 2A, and interacts with the CAP-Gsubunit of the condensin enzyme to promote efficient dephosphorylationand inactivation of condensin complexes in the vicinity, therebypreventing compaction at this site. Blocking HSF2-mediated bookmarkingby HSF2 RNA interference decreases hsp70i induction and survival ofstressed cells in the G[sub 1] phase, which demonstrates the biologicalimportance of gene bookmarking. [ABSTRACT FROM AUTHOR] |
| Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
|
Full text is not displayed to guests.
Login for full access.
|
|
| Abstract: | In contrast to most genomic DNA in mitotic cells, the promoterregions of some genes, such as the stress-inducible hsp70i gene thatcodes for a heat shock protein, remain uncompacted, a phenomenon calledbookmarking. Here we show that hsp70i bookmarking is mediated by atranscription factor called HSF2, which binds this promoter in mitoticcells, recruits protein phosphatase 2A, and interacts with the CAP-Gsubunit of the condensin enzyme to promote efficient dephosphorylationand inactivation of condensin complexes in the vicinity, therebypreventing compaction at this site. Blocking HSF2-mediated bookmarkingby HSF2 RNA interference decreases hsp70i induction and survival ofstressed cells in the G[sub 1] phase, which demonstrates the biologicalimportance of gene bookmarking. [ABSTRACT FROM AUTHOR] |
|---|---|
| ISSN: | 00368075 |
| DOI: | 10.1126/science.1106478 |
