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Effects of 30 days bed rest and exercise countermeasures on PBMC bioenergetics.

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Title: Effects of 30 days bed rest and exercise countermeasures on PBMC bioenergetics.
Authors: Buescher, F.‐M. (AUTHOR), Schmitz, M. T. (AUTHOR), Frett, T. (AUTHOR), Kramme, J. (AUTHOR), de Boni, L. (AUTHOR), Elmenhorst, E. M. (AUTHOR), Mulder, E. (AUTHOR), Moestl, S. (AUTHOR), Heusser, K. (AUTHOR), Frings‐Meuthen, P. (AUTHOR), Jordan, J. (AUTHOR), Rittweger, J. (AUTHOR), Pesta, D. (AUTHOR)
Source: Acta Physiologica. Mar2024, Vol. 240 Issue 3, p1-13. 13p.
Subjects: Bed rest, Bioenergetics, Mononuclear leukocytes, Leukocytes, Sabbath
Abstract: Aim: Altered mitochondrial function across various tissues is a key determinant of spaceflight‐induced physical deconditioning. In comparison to tissue biopsies, blood cell bioenergetics holds promise as a systemic and more readily accessible biomarker, which was evaluated during head‐down tilt bed rest (HDTBR), an established ground‐based analog for spaceflight‐induced physiological changes in humans. More specifically, this study explored the effects of HDTBR and an exercise countermeasure on mitochondrial respiration in peripheral blood mononuclear cells (PBMCs). Methods: We subjected 24 healthy participants to a strict 30‐day HDTBR protocol. The control group (n = 12) underwent HDTBR only, while the countermeasure group (n = 12) engaged in regular supine cycling exercise followed by veno‐occlusive thigh cuffs post‐exercise for 6 h. We assessed routine blood parameters 14 days before bed rest, the respiratory capacity of PBMCs via high‐resolution respirometry, and citrate synthase activity 2 days before and at day 30 of bed rest. We confirmed PBMC composition by flow cytometry. Results: The change of the PBMC maximal oxidative phosphorylation capacity (OXPHOS) amounted to an 11% increase in the countermeasure group, while it decreased by 10% in the control group (p = 0.04). The limitation of OXPHOS increased in control only while other respiratory states were not affected by either intervention. Correlation analysis revealed positive associations between white blood cells, lymphocytes, and basophils with PBMC bioenergetics in both groups. Conclusion: This study reveals that a regular exercise countermeasure has a positive impact on PBMC mitochondrial function, confirming the potential application of blood cell bioenergetics for human spaceflight. [ABSTRACT FROM AUTHOR]
Copyright of Acta Physiologica is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Effects of 30 days bed rest and exercise countermeasures on PBMC bioenergetics.
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  Data: <searchLink fieldCode="AR" term="%22Buescher%2C+F%2E‐M%2E%22">Buescher, F.‐M.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Schmitz%2C+M%2E+T%2E%22">Schmitz, M. T.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Frett%2C+T%2E%22">Frett, T.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kramme%2C+J%2E%22">Kramme, J.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22de+Boni%2C+L%2E%22">de Boni, L.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Elmenhorst%2C+E%2E+M%2E%22">Elmenhorst, E. M.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mulder%2C+E%2E%22">Mulder, E.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Moestl%2C+S%2E%22">Moestl, S.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Heusser%2C+K%2E%22">Heusser, K.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Frings‐Meuthen%2C+P%2E%22">Frings‐Meuthen, P.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Jordan%2C+J%2E%22">Jordan, J.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Rittweger%2C+J%2E%22">Rittweger, J.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pesta%2C+D%2E%22">Pesta, D.</searchLink> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22Acta+Physiologica%22">Acta Physiologica</searchLink>. Mar2024, Vol. 240 Issue 3, p1-13. 13p.
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  Data: <searchLink fieldCode="DE" term="%22Bed+rest%22">Bed rest</searchLink><br /><searchLink fieldCode="DE" term="%22Bioenergetics%22">Bioenergetics</searchLink><br /><searchLink fieldCode="DE" term="%22Mononuclear+leukocytes%22">Mononuclear leukocytes</searchLink><br /><searchLink fieldCode="DE" term="%22Leukocytes%22">Leukocytes</searchLink><br /><searchLink fieldCode="DE" term="%22Sabbath%22">Sabbath</searchLink>
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  Data: Aim: Altered mitochondrial function across various tissues is a key determinant of spaceflight‐induced physical deconditioning. In comparison to tissue biopsies, blood cell bioenergetics holds promise as a systemic and more readily accessible biomarker, which was evaluated during head‐down tilt bed rest (HDTBR), an established ground‐based analog for spaceflight‐induced physiological changes in humans. More specifically, this study explored the effects of HDTBR and an exercise countermeasure on mitochondrial respiration in peripheral blood mononuclear cells (PBMCs). Methods: We subjected 24 healthy participants to a strict 30‐day HDTBR protocol. The control group (n = 12) underwent HDTBR only, while the countermeasure group (n = 12) engaged in regular supine cycling exercise followed by veno‐occlusive thigh cuffs post‐exercise for 6 h. We assessed routine blood parameters 14 days before bed rest, the respiratory capacity of PBMCs via high‐resolution respirometry, and citrate synthase activity 2 days before and at day 30 of bed rest. We confirmed PBMC composition by flow cytometry. Results: The change of the PBMC maximal oxidative phosphorylation capacity (OXPHOS) amounted to an 11% increase in the countermeasure group, while it decreased by 10% in the control group (p = 0.04). The limitation of OXPHOS increased in control only while other respiratory states were not affected by either intervention. Correlation analysis revealed positive associations between white blood cells, lymphocytes, and basophils with PBMC bioenergetics in both groups. Conclusion: This study reveals that a regular exercise countermeasure has a positive impact on PBMC mitochondrial function, confirming the potential application of blood cell bioenergetics for human spaceflight. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Acta Physiologica is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Type: general
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