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Different electrophysiological profiles of genetically labelled dopaminergic neurons in the mouse midbrain and olfactory bulb.

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Title: Different electrophysiological profiles of genetically labelled dopaminergic neurons in the mouse midbrain and olfactory bulb.
Authors: Lau, Maggy Yu Hei (AUTHOR), Gadiwalla, Sana (AUTHOR), Jones, Susan (AUTHOR), Galliano, Elisa (AUTHOR)
Source: European Journal of Neuroscience. Apr2024, Vol. 59 Issue 7, p1480-1499. 20p.
Subjects: Mesencephalon, Dopaminergic neurons, Action potentials, Substantia nigra, Reward (Psychology), Olfactory bulb, Electrophysiology
Abstract: Dopaminergic (DA) neurons play pivotal roles in diverse brain functions, spanning movement, reward processing and sensory perception. DA neurons are most abundant in the midbrain (Substantia Nigra pars compacta [SNC] and Ventral Tegmental Area [VTA]) and the olfactory bulb (OB) in the forebrain. Interestingly, a subtype of OB DA neurons is capable of regenerating throughout life, while a second class is exclusively born during embryonic development. Compelling evidence in SNC and VTA also indicates substantial heterogeneity in terms of morphology, connectivity and function. To further investigate this heterogeneity and directly compare form and function of midbrain and forebrain bulbar DA neurons, we performed immunohistochemistry and whole‐cell patch‐clamp recordings in ex vivo brain slices from juvenile DAT‐tdTomato mice. After confirming the penetrance and specificity of the dopamine transporter (DAT) Cre line, we compared soma shape, passive membrane properties, voltage sags and action potential (AP) firing across midbrain and forebrain bulbar DA subtypes. We found that each DA subgroup within midbrain and OB was highly heterogeneous, and that DA neurons across the two brain areas are also substantially different. These findings complement previous work in rats as well as gene expression and in vivo datasets, further questioning the existence of a single "dopaminergic" neuronal phenotype. [ABSTRACT FROM AUTHOR]
Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Label: Title
  Group: Ti
  Data: Different electrophysiological profiles of genetically labelled dopaminergic neurons in the mouse midbrain and olfactory bulb.
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  Data: <searchLink fieldCode="AR" term="%22Lau%2C+Maggy+Yu+Hei%22">Lau, Maggy Yu Hei</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Gadiwalla%2C+Sana%22">Gadiwalla, Sana</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Jones%2C+Susan%22">Jones, Susan</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Galliano%2C+Elisa%22">Galliano, Elisa</searchLink> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22European+Journal+of+Neuroscience%22">European Journal of Neuroscience</searchLink>. Apr2024, Vol. 59 Issue 7, p1480-1499. 20p.
– Name: Subject
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  Data: <searchLink fieldCode="DE" term="%22Mesencephalon%22">Mesencephalon</searchLink><br /><searchLink fieldCode="DE" term="%22Dopaminergic+neurons%22">Dopaminergic neurons</searchLink><br /><searchLink fieldCode="DE" term="%22Action+potentials%22">Action potentials</searchLink><br /><searchLink fieldCode="DE" term="%22Substantia+nigra%22">Substantia nigra</searchLink><br /><searchLink fieldCode="DE" term="%22Reward+%28Psychology%29%22">Reward (Psychology)</searchLink><br /><searchLink fieldCode="DE" term="%22Olfactory+bulb%22">Olfactory bulb</searchLink><br /><searchLink fieldCode="DE" term="%22Electrophysiology%22">Electrophysiology</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Dopaminergic (DA) neurons play pivotal roles in diverse brain functions, spanning movement, reward processing and sensory perception. DA neurons are most abundant in the midbrain (Substantia Nigra pars compacta [SNC] and Ventral Tegmental Area [VTA]) and the olfactory bulb (OB) in the forebrain. Interestingly, a subtype of OB DA neurons is capable of regenerating throughout life, while a second class is exclusively born during embryonic development. Compelling evidence in SNC and VTA also indicates substantial heterogeneity in terms of morphology, connectivity and function. To further investigate this heterogeneity and directly compare form and function of midbrain and forebrain bulbar DA neurons, we performed immunohistochemistry and whole‐cell patch‐clamp recordings in ex vivo brain slices from juvenile DAT‐tdTomato mice. After confirming the penetrance and specificity of the dopamine transporter (DAT) Cre line, we compared soma shape, passive membrane properties, voltage sags and action potential (AP) firing across midbrain and forebrain bulbar DA subtypes. We found that each DA subgroup within midbrain and OB was highly heterogeneous, and that DA neurons across the two brain areas are also substantially different. These findings complement previous work in rats as well as gene expression and in vivo datasets, further questioning the existence of a single "dopaminergic" neuronal phenotype. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1111/ejn.16239
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      – Code: eng
        Text: English
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      – SubjectFull: Action potentials
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      – SubjectFull: Substantia nigra
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      – SubjectFull: Olfactory bulb
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      – SubjectFull: Electrophysiology
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              Text: Apr2024
              Type: published
              Y: 2024
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