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Myonuclear Addition to Uninjured Laryngeal Myofibers in Adult Rabbits.

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Title: Myonuclear Addition to Uninjured Laryngeal Myofibers in Adult Rabbits.
Authors: Goding Jr., George S., Al-Sharif, Kais I., McLoon, Linda K.
Source: Annals of Otology, Rhinology & Laryngology. Jul2005, Vol. 114 Issue 7, p552-557. 6p.
Subjects: Larynx injuries, Satellite cells, Cell membranes, Muscles, Immunohistochemistry, Laboratory rabbits
Abstract: Objectives: In normal mature limb skeletal muscle satellite cells are quiescent and myonuclei do not divide after formation of their associated myofibers in the absence of injury. The possibility of myonuclear addition in uninjured laryngeal myofibers of adult rabbits was investigated in an immunohislochemical pilot study. Methods: Bromodeoxyuridine (brdU). a marker for cell division, was administered by intraperitoneal injection over a 12-hour period in rabbits. The number of brdU-positive myonuclei per myofiber was determined on cross sections through the thyroarytenoid (TA) and posterior cricoarytenoid (PCA) muscles. Results: In the TA muscle, 0.13% ±0.03% (mean ± SEM) of the myofibers counted had a brdU-positive nucleus. In the PCA muscle, 0.13% ±0.01% of the myofibers counted had a brdU-positive nucleus. Approximately 0.2% and 0.3% of the myofibers of the TA and PCA muscles, respectively, had brdU-positive satellite cells associated with them. Tibialis anterior and pectoralis major muscle controls were negative for brdU-positive myonuclei. Conclusions: These data support the possibility of continuous remodeling in uninjured adult laryngeal myofibers and accentuate the distinct nature of laryngeal muscle relative to limb skeletal muscle in the rabbit model. [ABSTRACT FROM AUTHOR]
Copyright of Annals of Otology, Rhinology & Laryngology is the property of Sage Publications Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Label: Title
  Group: Ti
  Data: Myonuclear Addition to Uninjured Laryngeal Myofibers in Adult Rabbits.
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  Data: <searchLink fieldCode="AR" term="%22Goding+Jr%2E%2C+George+S%2E%22">Goding Jr., George S.</searchLink><br /><searchLink fieldCode="AR" term="%22Al-Sharif%2C+Kais+I%2E%22">Al-Sharif, Kais I.</searchLink><br /><searchLink fieldCode="AR" term="%22McLoon%2C+Linda+K%2E%22">McLoon, Linda K.</searchLink>
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  Data: <searchLink fieldCode="JN" term="%22Annals+of+Otology%2C+Rhinology+%26+Laryngology%22">Annals of Otology, Rhinology & Laryngology</searchLink>. Jul2005, Vol. 114 Issue 7, p552-557. 6p.
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  Label: Subjects
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  Data: <searchLink fieldCode="DE" term="%22Larynx+injuries%22">Larynx injuries</searchLink><br /><searchLink fieldCode="DE" term="%22Satellite+cells%22">Satellite cells</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+membranes%22">Cell membranes</searchLink><br /><searchLink fieldCode="DE" term="%22Muscles%22">Muscles</searchLink><br /><searchLink fieldCode="DE" term="%22Immunohistochemistry%22">Immunohistochemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Laboratory+rabbits%22">Laboratory rabbits</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Objectives: In normal mature limb skeletal muscle satellite cells are quiescent and myonuclei do not divide after formation of their associated myofibers in the absence of injury. The possibility of myonuclear addition in uninjured laryngeal myofibers of adult rabbits was investigated in an immunohislochemical pilot study. Methods: Bromodeoxyuridine (brdU). a marker for cell division, was administered by intraperitoneal injection over a 12-hour period in rabbits. The number of brdU-positive myonuclei per myofiber was determined on cross sections through the thyroarytenoid (TA) and posterior cricoarytenoid (PCA) muscles. Results: In the TA muscle, 0.13% ±0.03% (mean ± SEM) of the myofibers counted had a brdU-positive nucleus. In the PCA muscle, 0.13% ±0.01% of the myofibers counted had a brdU-positive nucleus. Approximately 0.2% and 0.3% of the myofibers of the TA and PCA muscles, respectively, had brdU-positive satellite cells associated with them. Tibialis anterior and pectoralis major muscle controls were negative for brdU-positive myonuclei. Conclusions: These data support the possibility of continuous remodeling in uninjured adult laryngeal myofibers and accentuate the distinct nature of laryngeal muscle relative to limb skeletal muscle in the rabbit model. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of Annals of Otology, Rhinology & Laryngology is the property of Sage Publications Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1177/000348940511400711
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        Text: English
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      – SubjectFull: Larynx injuries
        Type: general
      – SubjectFull: Satellite cells
        Type: general
      – SubjectFull: Cell membranes
        Type: general
      – SubjectFull: Muscles
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      – SubjectFull: Immunohistochemistry
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      – SubjectFull: Laboratory rabbits
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      – TitleFull: Myonuclear Addition to Uninjured Laryngeal Myofibers in Adult Rabbits.
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            NameFull: Al-Sharif, Kais I.
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              Text: Jul2005
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              Y: 2005
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