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Description and functional validation of human enteroendocrine cell sensors.

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Title: Description and functional validation of human enteroendocrine cell sensors.
Authors: Beumer, Joep, Geurts, Maarten H., Geurts, Veerle, Andersson-Rolf, Amanda, Akkerman, Ninouk, Völlmy, Franziska, Krueger, Daniel, Busslinger, Georg A., Martínez-Silgado, Adriana, Boot, Charelle, Yousef Yengej, Fjodor A., Puschhof, Jens, Van de Wetering, Wiline J., Knoops, Kevin, López-Iglesias, Carmen, Peters, Peter J., Vivié, Judith A., Mooijman, Dylan, van Es, Johan H., Clevers, Hans
Source: Science (pre-March 2025). 10/18/2024, Vol. 386 Issue 6719, p341-348. 8p. 5 Diagrams.
Subjects: Glucagon-like peptide 1, Enteroendocrine cells, Insulin sensitivity, Gastrointestinal contents, Epithelial cells
Abstract: Enteroendocrine cells (EECs) are gut epithelial cells that respond to intestinal contents by secreting hormones, including the incretins glucagon-like peptide 1 (GLP-1) and gastric inhibitory protein (GIP), which regulate multiple physiological processes. Hormone release is controlled through metabolite-sensing proteins. Low expression, interspecies differences, and the existence of multiple EEC subtypes have posed challenges to the study of these sensors. We describe differentiation of stomach EECs to complement existing intestinal organoid protocols. CD200 emerged as a pan-EEC surface marker, allowing deep transcriptomic profiling from primary human tissue along the stomach-intestinal tract. We generated loss-of-function mutations in 22 receptors and subjected organoids to ligand-induced secretion experiments. We delineate the role of individual human EEC sensors in the secretion of hormones, including GLP-1. These represent potential pharmacological targets to influence appetite, bowel movement, insulin sensitivity, and mucosal immunity. [ABSTRACT FROM AUTHOR]
Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Psychology and Behavioral Sciences Collection
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  Data: Description and functional validation of human enteroendocrine cell sensors.
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  Data: <searchLink fieldCode="AR" term="%22Beumer%2C+Joep%22">Beumer, Joep</searchLink><br /><searchLink fieldCode="AR" term="%22Geurts%2C+Maarten+H%2E%22">Geurts, Maarten H.</searchLink><br /><searchLink fieldCode="AR" term="%22Geurts%2C+Veerle%22">Geurts, Veerle</searchLink><br /><searchLink fieldCode="AR" term="%22Andersson-Rolf%2C+Amanda%22">Andersson-Rolf, Amanda</searchLink><br /><searchLink fieldCode="AR" term="%22Akkerman%2C+Ninouk%22">Akkerman, Ninouk</searchLink><br /><searchLink fieldCode="AR" term="%22Völlmy%2C+Franziska%22">Völlmy, Franziska</searchLink><br /><searchLink fieldCode="AR" term="%22Krueger%2C+Daniel%22">Krueger, Daniel</searchLink><br /><searchLink fieldCode="AR" term="%22Busslinger%2C+Georg+A%2E%22">Busslinger, Georg A.</searchLink><br /><searchLink fieldCode="AR" term="%22Martínez-Silgado%2C+Adriana%22">Martínez-Silgado, Adriana</searchLink><br /><searchLink fieldCode="AR" term="%22Boot%2C+Charelle%22">Boot, Charelle</searchLink><br /><searchLink fieldCode="AR" term="%22Yousef+Yengej%2C+Fjodor+A%2E%22">Yousef Yengej, Fjodor A.</searchLink><br /><searchLink fieldCode="AR" term="%22Puschhof%2C+Jens%22">Puschhof, Jens</searchLink><br /><searchLink fieldCode="AR" term="%22Van+de+Wetering%2C+Wiline+J%2E%22">Van de Wetering, Wiline J.</searchLink><br /><searchLink fieldCode="AR" term="%22Knoops%2C+Kevin%22">Knoops, Kevin</searchLink><br /><searchLink fieldCode="AR" term="%22López-Iglesias%2C+Carmen%22">López-Iglesias, Carmen</searchLink><br /><searchLink fieldCode="AR" term="%22Peters%2C+Peter+J%2E%22">Peters, Peter J.</searchLink><br /><searchLink fieldCode="AR" term="%22Vivié%2C+Judith+A%2E%22">Vivié, Judith A.</searchLink><br /><searchLink fieldCode="AR" term="%22Mooijman%2C+Dylan%22">Mooijman, Dylan</searchLink><br /><searchLink fieldCode="AR" term="%22van+Es%2C+Johan+H%2E%22">van Es, Johan H.</searchLink><br /><searchLink fieldCode="AR" term="%22Clevers%2C+Hans%22">Clevers, Hans</searchLink>
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  Data: <searchLink fieldCode="JN" term="%22Science+%28pre-March+2025%29%22">Science (pre-March 2025)</searchLink>. 10/18/2024, Vol. 386 Issue 6719, p341-348. 8p. 5 Diagrams.
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  Data: <searchLink fieldCode="DE" term="%22Glucagon-like+peptide+1%22">Glucagon-like peptide 1</searchLink><br /><searchLink fieldCode="DE" term="%22Enteroendocrine+cells%22">Enteroendocrine cells</searchLink><br /><searchLink fieldCode="DE" term="%22Insulin+sensitivity%22">Insulin sensitivity</searchLink><br /><searchLink fieldCode="DE" term="%22Gastrointestinal+contents%22">Gastrointestinal contents</searchLink><br /><searchLink fieldCode="DE" term="%22Epithelial+cells%22">Epithelial cells</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Enteroendocrine cells (EECs) are gut epithelial cells that respond to intestinal contents by secreting hormones, including the incretins glucagon-like peptide 1 (GLP-1) and gastric inhibitory protein (GIP), which regulate multiple physiological processes. Hormone release is controlled through metabolite-sensing proteins. Low expression, interspecies differences, and the existence of multiple EEC subtypes have posed challenges to the study of these sensors. We describe differentiation of stomach EECs to complement existing intestinal organoid protocols. CD200 emerged as a pan-EEC surface marker, allowing deep transcriptomic profiling from primary human tissue along the stomach-intestinal tract. We generated loss-of-function mutations in 22 receptors and subjected organoids to ligand-induced secretion experiments. We delineate the role of individual human EEC sensors in the secretion of hormones, including GLP-1. These represent potential pharmacological targets to influence appetite, bowel movement, insulin sensitivity, and mucosal immunity. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Science (pre-March 2025) is the property of American Association for the Advancement of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=pbh&AN=180320139
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        Value: 10.1126/science.adl1460
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        Text: English
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      – SubjectFull: Enteroendocrine cells
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      – SubjectFull: Insulin sensitivity
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      – SubjectFull: Gastrointestinal contents
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              Text: 10/18/2024
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