Cross-evaluating Epstein-Barr virus, human papilloma virus, human cytomegalovirus and human adenovirus in nasal polyps and turbinate mucosa.
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| Title: | Cross-evaluating Epstein-Barr virus, human papilloma virus, human cytomegalovirus and human adenovirus in nasal polyps and turbinate mucosa. |
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| Authors: | Schindele, Alexandra (AUTHOR), Holm, Anna (AUTHOR), Kraft, Sofie (AUTHOR), Nylander, Karin (AUTHOR), Allard, Annika (AUTHOR), Olofsson, Katarina (AUTHOR) |
| Source: | Acta Oto-Laryngologica. Feb2025, Vol. 145 Issue 2, p164-167. 4p. |
| Subjects: | Cross-sectional method, Research funding, Data analysis, Cytomegaloviruses, Adenoviruses, Papillomaviruses, Sinusitis, Descriptive statistics, Epstein-Barr virus, Nasal polyps, Nasal mucosa, Longitudinal method, Research methodology, Virus diseases, Data analysis software |
| Abstract (English): | Background: Chronic rhinosinusitis with nasal polyps (CRSwNP) is a common disease in which inflammatory responses to exogenic stressors, such as viral infections, has been recognised. The role of viruses in CRSwNP pathogenesis is unclear. Aims/objectives: We aimed to characterise Epstein-Barr virus (EBV), human papillomavirus (HPV), human cytomegalovirus (HCMV), and human adenovirus (HAdV) in nasal polyps and adjacent paired healthy turbinate mucosa. Materials and methods: We used real-time PCR for EBV, HCMV, and HAdV DNA detection, combined PCR/microarrays for HPV detection and genotyping, in samples from 45 patients with CRSwNP. Additionally, we used EBER in situ hybridisation for EBV detection. Results: EBV detection with EBER-ISH was significantly higher in polyps (36%) versus turbinate mucosa (12%). None of the viral comparisons with PCR between polyps and turbinate mucosa for EBV-, HCMV- or HAdV-DNA showed statistically significant differences. All samples were HPV negative. Conclusions and significance: We report higher expression of EBV in nasal polyps (36%) than in adjacent healthy turbinate mucosa (12%), using a valid method; EBER-ISH in 45 patients with CRSwNP. EBV might be a possible stressor that can trigger polypoid inflammation. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): | 慢性鼻窦炎伴鼻息肉 (CRSwNP) 是一种常见疾病, 已经认识到该病的对病毒感染等外部应激源的炎症反应。病毒的 CRSwNP 发病机制作用尚不清楚。 我们旨在确定鼻息肉和相邻的健康鼻甲粘膜中的 Epstein-Barr 病毒 (EBV)、人乳头瘤病毒 (HPV)、人类巨细胞病毒 (HCMV) 和人类腺病毒 (HAdV)的特征。 我们对 45 名 CRSwNP 患者的样本使用实时 PCR 来检测 EBV、HCMV 和 HAdV DNA, 综合运用 PCR/微阵列检测 HPV 和基因分型。此外, 我们使用 EBER 原位杂交来检测 EBV。 使用 EBER-ISH 检测息肉 (36%) 中的 EBV 明显高于鼻甲粘膜 (12%)。使用 PCR 对息肉和鼻甲粘膜进行 EBV-、HCMV 或 HAdV-DNA 病毒比较, 均未显示统计学上显著差异。所有样本均为 HPV 阴性。 我们使用一种有效的方法, 发现鼻息肉 (36%) 中的 EBV 表达高于邻近健康鼻甲粘膜 (12%)； 45 名 CRSwNP 患者携带 EBER-ISH。EBV 可能是引发息肉样炎症的潜在应激源。 [ABSTRACT FROM AUTHOR] |
| Copyright of Acta Oto-Laryngologica is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
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| Abstract: | Background: Chronic rhinosinusitis with nasal polyps (CRSwNP) is a common disease in which inflammatory responses to exogenic stressors, such as viral infections, has been recognised. The role of viruses in CRSwNP pathogenesis is unclear. Aims/objectives: We aimed to characterise Epstein-Barr virus (EBV), human papillomavirus (HPV), human cytomegalovirus (HCMV), and human adenovirus (HAdV) in nasal polyps and adjacent paired healthy turbinate mucosa. Materials and methods: We used real-time PCR for EBV, HCMV, and HAdV DNA detection, combined PCR/microarrays for HPV detection and genotyping, in samples from 45 patients with CRSwNP. Additionally, we used EBER in situ hybridisation for EBV detection. Results: EBV detection with EBER-ISH was significantly higher in polyps (36%) versus turbinate mucosa (12%). None of the viral comparisons with PCR between polyps and turbinate mucosa for EBV-, HCMV- or HAdV-DNA showed statistically significant differences. All samples were HPV negative. Conclusions and significance: We report higher expression of EBV in nasal polyps (36%) than in adjacent healthy turbinate mucosa (12%), using a valid method; EBER-ISH in 45 patients with CRSwNP. EBV might be a possible stressor that can trigger polypoid inflammation. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 00016489 |
| DOI: | 10.1080/00016489.2024.2445025 |
