Anatomical mapping of neural lineages expressing the transient receptor potential vanilloid type 1 receptor using a modified and combined PACT and CUBIC protocol for rapid tissue clearance.
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| Title: | Anatomical mapping of neural lineages expressing the transient receptor potential vanilloid type 1 receptor using a modified and combined PACT and CUBIC protocol for rapid tissue clearance. |
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| Authors: | Kitzerow, Oliver (AUTHOR), Christensen, Samuel (AUTHOR), Hong, Juan (AUTHOR), Adam, Ryan J. (AUTHOR), Zucker, Irving H. (AUTHOR), Jensen‐Smith, Heather (AUTHOR), Wang, Han‐Jun (AUTHOR) |
| Source: | Acta Physiologica. Feb2025, Vol. 241 Issue 2, p1-14. 14p. |
| Subjects: | TRPV cation channels, Three-dimensional imaging, Fate mapping (Genetics), Brain anatomy, Preservation of organs, tissues, etc. |
| Abstract: | Aim: Tissue clearance is a rapidly evolving technology that allows for the three‐dimensional imaging of intact biological tissues. Preexisting tissue‐clearing techniques, such as Passive Clarity Technique (PACT) and Clear Unobstructed Brain Imaging Cocktails and Computational Analysis (CUBIC), clear tissues adequately but have distinct disadvantages, such as taking extensive time to clear tissues and degradation of endogenous tissue fluorescence. We developed a new tissue‐clearing technique combining PACT and CUBIC protocols to map the neural lineages expressing the transient receptor potential vanilloid type 1 (TRPV1) receptor. Methods: To test the effectiveness of this modified protocol, a TdTomato reporter mouse line was crossed with a separate mouse line containing Cre recombinase under the control of the TRPV1 promoter, which would result in TRPV1 cell lineages expressing green fluorescence protein (GFP). Results: Compared to the PACT protocol that requires several weeks to months for tissue clearance, our approach reached a satisfactory clearance within 3 days in all neural tissues as well as several non‐neural tissues such as colon, duodenum, and pancreas. Compared to the CUBIC approach, all tissues reserved strong GFP fluorescence. Robust GFP fluorescence was visualized in sensory neuronal soma but not in sympathetic ganglia neuronal soma. On the other hand, GFP fluorescence in the TRPV1 cells appeared to be expressed throughout the epithelium of the duodenum and colon and the arteriole smooth muscle in all non‐neuronal tissues. Conclusion: This study shows that our combined PACT and CUBIC (CPC) protocol can clear tissues in significantly less time while preserving tissue integrity and fluorescence. [ABSTRACT FROM AUTHOR] |
| Copyright of Acta Physiologica is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Psychology and Behavioral Sciences Collection |
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| Header | DbId: pbh DbLabel: Psychology and Behavioral Sciences Collection An: 183844927 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Anatomical mapping of neural lineages expressing the transient receptor potential vanilloid type 1 receptor using a modified and combined PACT and CUBIC protocol for rapid tissue clearance. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Kitzerow%2C+Oliver%22">Kitzerow, Oliver</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Christensen%2C+Samuel%22">Christensen, Samuel</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hong%2C+Juan%22">Hong, Juan</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Adam%2C+Ryan+J%2E%22">Adam, Ryan J.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zucker%2C+Irving+H%2E%22">Zucker, Irving H.</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Jensen‐Smith%2C+Heather%22">Jensen‐Smith, Heather</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Han‐Jun%22">Wang, Han‐Jun</searchLink> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Acta+Physiologica%22">Acta Physiologica</searchLink>. Feb2025, Vol. 241 Issue 2, p1-14. 14p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22TRPV+cation+channels%22">TRPV cation channels</searchLink><br /><searchLink fieldCode="DE" term="%22Three-dimensional+imaging%22">Three-dimensional imaging</searchLink><br /><searchLink fieldCode="DE" term="%22Fate+mapping+%28Genetics%29%22">Fate mapping (Genetics)</searchLink><br /><searchLink fieldCode="DE" term="%22Brain+anatomy%22">Brain anatomy</searchLink><br /><searchLink fieldCode="DE" term="%22Preservation+of+organs%2C+tissues%2C+etc%2E%22">Preservation of organs, tissues, etc.</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Aim: Tissue clearance is a rapidly evolving technology that allows for the three‐dimensional imaging of intact biological tissues. Preexisting tissue‐clearing techniques, such as Passive Clarity Technique (PACT) and Clear Unobstructed Brain Imaging Cocktails and Computational Analysis (CUBIC), clear tissues adequately but have distinct disadvantages, such as taking extensive time to clear tissues and degradation of endogenous tissue fluorescence. We developed a new tissue‐clearing technique combining PACT and CUBIC protocols to map the neural lineages expressing the transient receptor potential vanilloid type 1 (TRPV1) receptor. Methods: To test the effectiveness of this modified protocol, a TdTomato reporter mouse line was crossed with a separate mouse line containing Cre recombinase under the control of the TRPV1 promoter, which would result in TRPV1 cell lineages expressing green fluorescence protein (GFP). Results: Compared to the PACT protocol that requires several weeks to months for tissue clearance, our approach reached a satisfactory clearance within 3 days in all neural tissues as well as several non‐neural tissues such as colon, duodenum, and pancreas. Compared to the CUBIC approach, all tissues reserved strong GFP fluorescence. Robust GFP fluorescence was visualized in sensory neuronal soma but not in sympathetic ganglia neuronal soma. On the other hand, GFP fluorescence in the TRPV1 cells appeared to be expressed throughout the epithelium of the duodenum and colon and the arteriole smooth muscle in all non‐neuronal tissues. Conclusion: This study shows that our combined PACT and CUBIC (CPC) protocol can clear tissues in significantly less time while preserving tissue integrity and fluorescence. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Acta Physiologica is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1111/apha.14275 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 14 StartPage: 1 Subjects: – SubjectFull: TRPV cation channels Type: general – SubjectFull: Three-dimensional imaging Type: general – SubjectFull: Fate mapping (Genetics) Type: general – SubjectFull: Brain anatomy Type: general – SubjectFull: Preservation of organs, tissues, etc. Type: general Titles: – TitleFull: Anatomical mapping of neural lineages expressing the transient receptor potential vanilloid type 1 receptor using a modified and combined PACT and CUBIC protocol for rapid tissue clearance. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Kitzerow, Oliver – PersonEntity: Name: NameFull: Christensen, Samuel – PersonEntity: Name: NameFull: Hong, Juan – PersonEntity: Name: NameFull: Adam, Ryan J. – PersonEntity: Name: NameFull: Zucker, Irving H. – PersonEntity: Name: NameFull: Jensen‐Smith, Heather – PersonEntity: Name: NameFull: Wang, Han‐Jun IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 02 Text: Feb2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 17481708 Numbering: – Type: volume Value: 241 – Type: issue Value: 2 Titles: – TitleFull: Acta Physiologica Type: main |
| ResultId | 1 |
