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L1CAM and p65 as Predictive Markers of ZFTA Fusion in Ependymomas.

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Title: L1CAM and p65 as Predictive Markers of ZFTA Fusion in Ependymomas.
Authors: Turker Saricoban, Cansu (AUTHOR), Akar, Kadriye Ebru (AUTHOR), Onenerk, Ayse Mine (AUTHOR), Uyar Bozkurt, Suheyla (AUTHOR), Batur, Sebnem (AUTHOR), Comunoglu, Nil (AUTHOR), Oz, Buge (AUTHOR), Subramanian, Senthilkumar (AUTHOR)
Source: Acta Neurologica Scandinavica. 6/4/2025, Vol. 2025, p1-10. 10p.
Subjects: Ependymoma, Immunohistochemistry, Health Organisation, Prognosis, Cell adhesion molecules, Gene fusion, NF-kappa B, Fluorescence in situ hybridization, Nosology
Abstract: Purpose: In the 2021 World Health Organization (WHO) classification, ependymomas were classified according to their anatomical localization and molecular features. The "RELA fusion‐positive ependymoma" group, which was the first defined molecular subtype in the previous (2016) classification, was included as "ZFTA fusion‐positive ependymoma" in the final classification. We aimed to determine ZFTA fusion‐positivity in supratentorial (ST) and posterior fossa (PF) ependymomas and to investigate the correlation with both L1CAM and p65 immunoreactivity. Methods: The study included 17 ST and 9 PF cases with Grades 1, 2, and 3 ependymomas. The presence of the ZFTA fusion was evaluated using the fluorescence in situ hybridization (FISH) technique. L1CAM and p65 antibodies were applied for immunohistochemical analysis. The immunoreactivity for L1CAM and p65 was correlated with ZFTA fusion as assessed by FISH. Prognostic significance of the same was evaluated using the Kaplan–Meier survival analysis. Results: ZFTA fusion‐positivity was detected in 7 of 12 (58%) ST‐localized Grade 3 ependymoma cases; however, it was not observed in PF‐localized cases or in ST‐localized Grade 1 subependymoma (SE) and Grade 2 ependymoma cases. Six of the seven ZFTA fusion‐positive cases exhibited clear cell morphology. All ZFTA fusion‐positive cases showed L1CAM immunohistochemical positivity, and six of them also demonstrated nuclear p65 positivity. Moreover, we identified a new FISH pattern, which we termed the "short break‐apart." Conclusion: Together, these data indicate a strong correlation between FISH and immunohistochemistry results. However, a more reliable assessment on this matter could be accomplished through a multicentric study involving a larger number of cases. [ABSTRACT FROM AUTHOR]
Copyright of Acta Neurologica Scandinavica is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: L1CAM and p65 as Predictive Markers of ZFTA Fusion in Ependymomas.
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  Data: <searchLink fieldCode="AR" term="%22Turker+Saricoban%2C+Cansu%22">Turker Saricoban, Cansu</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Akar%2C+Kadriye+Ebru%22">Akar, Kadriye Ebru</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Onenerk%2C+Ayse+Mine%22">Onenerk, Ayse Mine</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Uyar+Bozkurt%2C+Suheyla%22">Uyar Bozkurt, Suheyla</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Batur%2C+Sebnem%22">Batur, Sebnem</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Comunoglu%2C+Nil%22">Comunoglu, Nil</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Oz%2C+Buge%22">Oz, Buge</searchLink> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Subramanian%2C+Senthilkumar%22">Subramanian, Senthilkumar</searchLink> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22Acta+Neurologica+Scandinavica%22">Acta Neurologica Scandinavica</searchLink>. 6/4/2025, Vol. 2025, p1-10. 10p.
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  Data: <searchLink fieldCode="DE" term="%22Ependymoma%22">Ependymoma</searchLink><br /><searchLink fieldCode="DE" term="%22Immunohistochemistry%22">Immunohistochemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Health+Organisation%22">Health Organisation</searchLink><br /><searchLink fieldCode="DE" term="%22Prognosis%22">Prognosis</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+adhesion+molecules%22">Cell adhesion molecules</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+fusion%22">Gene fusion</searchLink><br /><searchLink fieldCode="DE" term="%22NF-kappa+B%22">NF-kappa B</searchLink><br /><searchLink fieldCode="DE" term="%22Fluorescence+in+situ+hybridization%22">Fluorescence in situ hybridization</searchLink><br /><searchLink fieldCode="DE" term="%22Nosology%22">Nosology</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Purpose: In the 2021 World Health Organization (WHO) classification, ependymomas were classified according to their anatomical localization and molecular features. The "RELA fusion‐positive ependymoma" group, which was the first defined molecular subtype in the previous (2016) classification, was included as "ZFTA fusion‐positive ependymoma" in the final classification. We aimed to determine ZFTA fusion‐positivity in supratentorial (ST) and posterior fossa (PF) ependymomas and to investigate the correlation with both L1CAM and p65 immunoreactivity. Methods: The study included 17 ST and 9 PF cases with Grades 1, 2, and 3 ependymomas. The presence of the ZFTA fusion was evaluated using the fluorescence in situ hybridization (FISH) technique. L1CAM and p65 antibodies were applied for immunohistochemical analysis. The immunoreactivity for L1CAM and p65 was correlated with ZFTA fusion as assessed by FISH. Prognostic significance of the same was evaluated using the Kaplan–Meier survival analysis. Results: ZFTA fusion‐positivity was detected in 7 of 12 (58%) ST‐localized Grade 3 ependymoma cases; however, it was not observed in PF‐localized cases or in ST‐localized Grade 1 subependymoma (SE) and Grade 2 ependymoma cases. Six of the seven ZFTA fusion‐positive cases exhibited clear cell morphology. All ZFTA fusion‐positive cases showed L1CAM immunohistochemical positivity, and six of them also demonstrated nuclear p65 positivity. Moreover, we identified a new FISH pattern, which we termed the "short break‐apart." Conclusion: Together, these data indicate a strong correlation between FISH and immunohistochemistry results. However, a more reliable assessment on this matter could be accomplished through a multicentric study involving a larger number of cases. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Acta Neurologica Scandinavica is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1155/ane/1281170
    Languages:
      – Code: eng
        Text: English
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        PageCount: 10
        StartPage: 1
    Subjects:
      – SubjectFull: Ependymoma
        Type: general
      – SubjectFull: Immunohistochemistry
        Type: general
      – SubjectFull: Health Organisation
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      – SubjectFull: Prognosis
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      – SubjectFull: Cell adhesion molecules
        Type: general
      – SubjectFull: Gene fusion
        Type: general
      – SubjectFull: NF-kappa B
        Type: general
      – SubjectFull: Fluorescence in situ hybridization
        Type: general
      – SubjectFull: Nosology
        Type: general
    Titles:
      – TitleFull: L1CAM and p65 as Predictive Markers of ZFTA Fusion in Ependymomas.
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              M: 06
              Text: 6/4/2025
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              Y: 2025
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